164 research outputs found
Pectinase-hyperproducing mutants of Aspergillus niger C28B25 for solid-state fermentation of coffee pulp
The aim of this study was to improve mold strains for the production of pectinases by solid-state fermentation (SSF) of coffee pulp. A screening of 248 strains, isolated in Mexico's coffee-growing areas, permitted us to select a wild strain of #Aspergillus niger$ which in 72 h attains a peak production of 27.7 U ml-1(138 U g-1 dry pulp) of pectinase measured by viscosimetry. Though the use of a selective culture medium with low water activity (aw = 0.954) with 2-deoxy-glucose (2DG) it was possible to isolate pectinase-hyperproductive mutant strains for SSF(44.5 U ml-1, 228 U g-1 dry pulp). Derepressed mutant strains that hyperproduced pectinase by submerged fermentation (SmF) were also obtained using a classic selective medium with high water activity (pectin + 2DG) with aw = 0.999. A comparison between both classes of mutants, called AW96 and AW99, respectively, points out the need to design special selective media in order to obtain strains adapted either to SSF or Smf in which the aw level would be a key selecting factor. (Résumé d'auteur
Utilization of slaughter-house by products as nitrogen source for filamentous fungi in submerged fermentation, with simultaneous deodorization
Massless Metric Preheating
Can super-Hubble metric perturbations be amplified exponentially during
preheating ? Yes. An analytical existence proof is provided by exploiting the
conformal properties of massless inflationary models. The traditional conserved
quantity \zeta is non-conserved in many regions of parameter space. We include
backreaction through the homogeneous parts of the inflaton and preheating
fields and discuss the role of initial conditions on the post-preheating
power-spectrum. Maximum field variances are strongly underestimated if metric
perturbations are ignored. We illustrate this in the case of strong
self-interaction of the decay products. Without metric perturbations,
preheating in this case is very inefficient. However, metric perturbations
increase the maximum field variances and give alternative channels for the
resonance to proceed. This implies that metric perturbations can have a large
impact on calculations of relic abundances of particles produced during
preheating.Comment: 8 pages, 4 colour figures. Version to appear in Phys. Rev. D.
Contains substantial new analysis of the ranges of parameter space for which
large changes to the inflation-produced power spectrum are expecte
Phosphorylation of myosin light chain from adrenomedullary chromaffin cells in culture
Chronic Exposure to Arsenic and Markers of Cardiometabolic Risk: A Cross-Sectional Study in Chihuahua, Mexico
BackgroundExposure to arsenic (As) concentrations in drinking water > 150 μg/L has been associated with risk of diabetes and cardiovascular disease, but little is known about the effects of lower exposures.ObjectiveThis study aimed to examine whether moderate As exposure, or indicators of individual As metabolism at these levels of exposure, are associated with cardiometabolic risk.MethodsWe analyzed cross-sectional associations between arsenic exposure and multiple markers of cardiometabolic risk using drinking-water As measurements and urinary As species data obtained from 1,160 adults in Chihuahua, Mexico, who were recruited in 2008–2013. Fasting blood glucose and lipid levels, the results of an oral glucose tolerance test, and blood pressure were used to characterize cardiometabolic risk. Multivariable logistic, multinomial, and linear regression were used to assess associations between cardiometabolic outcomes and water As or the sum of inorganic and methylated As species in urine.ResultsAfter multivariable adjustment, concentrations in the second quartile of water As (25.5 to < 47.9 μg/L) and concentrations of total speciated urinary As (< 55.8 μg/L) below the median were significantly associated with elevated triglycerides, high total cholesterol, and diabetes. However, moderate water and urinary As levels were also positively associated with HDL cholesterol. Associations between arsenic exposure and both dysglycemia and triglyceridemia were higher among individuals with higher proportions of dimethylarsenic in urine.ConclusionsModerate exposure to As may increase cardiometabolic risk, particularly in individuals with high proportions of urinary dimethylarsenic. In this cohort, As exposure was associated with several markers of increased cardiometabolic risk (diabetes, triglyceridemia, and cholesterolemia), but exposure was also associated with higher rather than lower HDL cholesterol.CitationMendez MA, González-Horta C, Sánchez-RamÃrez B, Ballinas-Casarrubias L, Hernández Cerón R, Viniegra Morales D, Baeza Terrazas FA, Ishida MC, Gutiérrez-Torres DS, Saunders RJ, Drobná Z, Fry RC, Buse JB, Loomis D, GarcÃa-Vargas GG, Del Razo LM, Stýblo M. 2016. Chronic exposure to arsenic and markers of cardiometabolic risk: a cross-sectional study in Chihuahua, Mexico. Environ Health Perspect 124:104–111; http://dx.doi.org/10.1289/ehp.140874
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