Determinación de la composición química de piezas metálicas históricas: aplicación a un bronce romano

La deicrniinación de la composición química media de un bronce arqueoló gico no siempre es fácil. Algunos problemas a resolver son la heterogeneidad de la composición química, la dificultad de extracción de muestras o la importan cia de los elementos traza. En la comunicación se discute en primer lugar la me todología y técnicas de análisis apropiadas para la determinación de la compo sición de bronces singulares. Por último, se describe un procedimiento de obtención de viruta, cuasi no destructivo, para realizar análisis por procedimientos con vencionales. La validez y el significado de los resultados se discuten mediante la aplicación del procedimiento a un bronce de origen romano: el Hypnos ha llado en la villa romana de El Ruedo, en Almedinilla (Córdoba)

Characterization of Human Herpesvirus 8 genomic integration and amplification events in a primary effusion lymphoma cell line

In this study, we investigated the integration of Human Herpesvirus 8 (HHV-8) into the human genome using the primary effusion lymphoma (PEL) cell line BC-3. Through next-generation sequencing (NGS) data from multiple independent sequencing runs, we identified two highly supported HHV-8 integrants. These integrants encompassed a region of human chromosome 12 that was amplified approximately 16-fold between the junctions. Significantly, these events could represent the first known instance of HHV-8 integration into a hybrid human-viral extrachromosomal chimeric circular DNA (eccDNA). The amplified fragment contained partial or complete copies of various human genes, including SELPLG and CORO1C. Analysis of long-read Nanopore data indicated that the CpGs at the SELPLG promoter were mostly unmethylated, suggesting that the additional copies of SELPLG within this eccDNA are likely transcriptionally active. Our findings suggest that viral insertion and eccDNA amplification could be crucial mechanisms in the development of HHV-8-related cancers. In conclusion, our study provides valuable insights into the molecular mechanisms involved in HHV-8-induced oncogenesis and emphasizes the importance of investigating viral integration and eccDNAs in cancer development. Furthermore, we highlight the necessity of employing multiple independent sequencing approaches to validate integration events and avoid false positives derived from library construction artifacts

Determinación de la composición química de piezas metálicas históricas: aplicación a un bronce romano

La determinación de la composición química media de un bronce arqueológico no siempre es fácil. Algunos problemas a resolver son la heterogeneidad de la composición química, la dificultad de extracción de muestras o la importancia de los elementos traza. En la comunicación se discute en primer lugar la metodología y técnicas de análisis apropiadas para la determinación de la composición de bronces singulares. Por último, se describe un procedimiento de obtención de viruta, cuasi no destructivo, para realizar análisis por procedimientos convencionales. La validez y el significado de los resultados se discuten mediante la aplicación del procedimiento a un bronce de origen romano: el Hypnos hallado en la villa romana de El Ruedo, en Almedinilla (Córdoba)

Ty3/Gypsy retrotransposons in the Pacific abalone Haliotis discus hannai: characterization and use for species identification in the genus Haliotis

Transposable elements are highly abundant elements that are present in all eukaryotic species. Here, we present a molecular description of abalone retrotransposon (Abret) elements. The genome of Haliotis discus hannai contains 130 Abret elements which were all Ty3/Gypsy retrotransposons. The Ty1/Copia elements were absent in the H. discus hannai genome. Most of the elements were not complete due to sequence truncation or coding region decay. However, three elements Abret-296, Abret-935, and Abret-3259 had most of the canonical features of LTR (long terminal repeat)-retrotransposons. There were several reading frame shifts in Abret-935 and Abret-3259 elements. Surprisingly, phylogenetic analysis indicated that all of the elements belonged to the Osvaldo lineage. The sequence divergence between LTRs revealed that the Abret elements were mostly active within 2 million years ago. Abret elements were used as molecular markers in SSAP analyses, which allowed clear distinction of different species in the genus Haliotis. The polymorphic markers were converted into SCAR markers for use in species identification by simple PCR in the Haliotis genus