38 research outputs found
Site-specific modification of the lactose operator with acetylaminofluorene.
We have synthesized the tetradecamer GAGCXGATAACAAG containing a part of the sequence of the lactose operator. A guanine base in the sequence is replaced by the adduct of the carcinogen 2-acetylaminofluorene with guanine. Under the standard conditions of de-protection, the fluorene moiety is lost, leaving behind a guanine oxidation product. New conditions of de-protection have been developed which allow the isolation of an oligonucleotide containing the adduct of 2-aminofluorene with guanine. The presence of the aminofluorene adduct greatly increases retention on reverse phase chromatography and produces a unique pattern of sequencing bands
Identification of two new metabolites of caffeine in the rat urine
L'acide 1, 3, 7-triméthyldihydro-urique et la 3, 6, 8-triméthylallantoïne ont été identifiés comme métabolites nouveaux de la cafféine dans l'urine de rat.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/42553/1/18_2005_Article_BF01930396.pd