Cancer therapy-induced PAFR ligand expression: any role for caspase activity?

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Beyond complex Langevin equations II: a positive representation of Feynman path integrals directly in the Minkowski time

Recently found positive representation for an arbitrary complex, gaussian weight is used to construct a statistical formulation of gaussian path integrals directly in the Minkowski time. The positivity of Minkowski weights is achieved by doubling the number of real variables. The continuum limit of the new representation exists only if some of the additional couplings tend to infinity and are tuned in a specific way. The construction is then successfully applied to three quantum mechanical examples including a particle in a constant magnetic field -- a simplest prototype of a Wilson line. Further generalizations are shortly discussed and an intriguing interpretation of new variables is alluded to.Comment: 16 pages, 2 figures, references adde

Antichain cutsets of strongly connected posets

Rival and Zaguia showed that the antichain cutsets of a finite Boolean lattice are exactly the level sets. We show that a similar characterization of antichain cutsets holds for any strongly connected poset of locally finite height. As a corollary, we get such a characterization for semimodular lattices, supersolvable lattices, Bruhat orders, locally shellable lattices, and many more. We also consider a generalization to strongly connected hypergraphs having finite edges.Comment: 12 pages; v2 contains minor fixes for publicatio

Class of invariants for the 2D time-dependent Landau problem and harmonic oscillator in a magnetic field

We consider an isotropic two dimensional harmonic oscillator with arbitrarily time-dependent mass $M(t)$ and frequency $\Omega(t)$ in an arbitrarily time-dependent magnetic field $B(t)$. We determine two commuting invariant observables (in the sense of Lewis and Riesenfeld) $L,I$ in terms of some solution of an auxiliary ordinary differential equation and an orthonormal basis of the Hilbert space consisting of joint eigenvectors $\phi_\lambda$ of $L,I$. We then determine time-dependent phases $\alpha_\lambda(t)$ such that the $\psi_\lambda(t)=e^{i\alpha_\lambda}\phi_\lambda$ are solutions of the time-dependent Schr\"odinger equation and make up an orthonormal basis of the Hilbert space. These results apply, in particular to a two dimensional Landau problem with time-dependent $M,B$, which is obtained from the above just by setting $\Omega(t) \equiv 0$. By a mere redefinition of the parameters, these results can be applied also to the analogous models on the canonical non-commutative plane.Comment: 13 pages, 3 references adde

Hybrid in vitro diffusion cell for simultaneous evaluation of hair and skin decontamination: temporal distribution of chemical contaminants

Most casualty or personnel decontamination studies have focused on removing contaminants from the skin. However, scalp hair and underlying skin are the most likely areas of contamination following airborne exposure to chemicals. The aim of this study was to investigate the interactions of contaminants with scalp hair and underlying skin using a hybrid in vitro diffusion cell model. The in vitro hybrid test system comprised “curtains” of human hair mounted onto sections of excised porcine skin within a modified diffusion cell. The results demonstrated that hair substantially reduced underlying scalp skin contamination and that hair may provide a limited decontamination effect by removing contaminants from the skin surface. This hybrid test system may have application in the development of improved chemical incident response processes through the evaluation of various hair and skin decontamination strategies.Peer reviewedFinal Published versio

Nasal Lipopolysaccharide Challenge and Cytokine Measurement Reflects Innate Mucosal Immune Responsiveness

<div><p>Background</p><p><b>P</b>ractical methods of monitoring innate immune mucosal responsiveness are lacking. Lipopolysaccharide (LPS) is a component of the cell wall of Gram negative bacteria and a potent activator of Toll-like receptor (TLR)-4. To measure LPS responsiveness of the nasal mucosa, we administered LPS as a nasal spray and quantified chemokine and cytokine levels in mucosal lining fluid (MLF).</p><p>Methods</p><p>We performed a 5-way cross-over, single blind, placebo-controlled study in 15 healthy non-atopic subjects (n = 14 <i>per protocol</i>). Doses of ultrapure LPS (1, 10, 30 or 100μg/100μl) or placebo were administered by a single nasal spray to each nostril. Using the recently developed method of nasosorption with synthetic adsorptive matrices (SAM), a series of samples were taken. A panel of seven cytokines/chemokines were measured by multiplex immunoassay in MLF. mRNA for intercellular cell adhesion molecule-1 (ICAM-1) was quantified from nasal epithelial curettage samples taken before and after challenge.</p><p>Results</p><p>Topical nasal LPS was well tolerated, causing no symptoms and no visible changes to the nasal mucosa. LPS induced dose-related increases in MLF levels of IL-1β, IL-6, CXCL8 (IL-8) and CCL3 (MIP-1α) (AUC at 0.5 to 10h, compared to placebo, p<0.05 at 30 and 100μg LPS). At 100μg LPS, IL-10, IFN-α and TNF-α were also increased (p<0.05). Dose-related changes in mucosal ICAM-1 mRNA were also seen after challenge, and neutrophils appeared to peak in MLF at 8h. However, 2 subjects with high baseline cytokine levels showed prominent cytokine and chemokine responses to relatively low LPS doses (10μg and 30μg LPS).</p><p>Conclusions</p><p>Topical nasal LPS causes dose-dependent increases in cytokines, chemokines, mRNA and cells. However, responsiveness can show unpredictable variations, possibly because baseline innate tone is affected by environmental factors. We believe that this new technique will have wide application in the study of the innate immune responses of the respiratory mucosa.</p><p>Key Messages</p><p>Ultrapure LPS was used as innate immune stimulus in a human nasal challenge model, with serial sampling of nasal mucosal lining fluid (MLF) by nasosorption using a synthetic absorptive matrix (SAM), and nasal curettage of mucosal cells. A dose response could be demonstrated in terms of levels of IL-1β, IL-6, CXCL8 and CCL3 in MLF, as well as ICAM-1 mRNA in nasal curettage specimens, and levels of neutrophils in nasal lavage. Depending on higher baseline levels of inflammation, there were occasional magnified innate inflammatory responses to LPS.</p><p>Trial Registration</p><p>Clinical Trials.gov <a href="https://clinicaltrials.gov/ct2/show/NCT02284074?term=nasal+lipopolysaccharide&rank=1" target="_blank">NCT02284074</a></p></div

Reversible viscosity and Navier--Stokes fluids

Exploring the possibility of describing a fluid flow via a time-reversible equation and its relevance for the fluctuations statistics in stationary turbulent (or laminar) incompressible Navier-Stokes flows.Comment: 7 pages 6 figures, v2: replaced Fig.6 and few changes. Last version: appendix cut shorter, because of a computational erro

New atomic data for C i Rydberg states compared with solar UV spectra

We use the Breit-Pauli R-matrix method to calculate accurate energies and radiative data for states in C i up to n = 30 and with l ≤ 3. We provide the full data set of decays to the five 2s2 2p2 ground configuration states 3P0,1,21D2, and 1S0. This is the first complete set of data for transitions from n ≥ 5. We compare oscillator strengths and transition probabilities with the few previously calculated values for such transitions, finding generally good agreement (within 10 per cent) with the exception of values recently recommended by National Institute of Standards and Technology, where significant discrepancies are found. We then calculate spectral line intensities originating from the Rydberg states using typical chromospheric conditions and assuming local thermal equilibrium, and compare them with well-calibrated Solar and Heliospheric Observatory Solar Ultraviolet Measurements of Emitted Radiation ultraviolet (UV) spectra of the quiet Sun. The relative intensities of the Rydberg series are in excellent agreement with observation, which provides firm evidence for the identifications and blends of nearly 200 UV lines. Such comparison also resulted in a large number of new identifications of C i lines in the spectra. We also estimate optical depth effects and find that these can account for much of the absorption noted in the observations. The atomic data can be applied to model a wide range of solar and astrophysical observations