Vermicomposting of coirpith with cowdung by Eudrilus eugeniae Kinberg and its efficacy on the growth of Cyamopsis tetragonaloba (L) Taub

AbstractIn view of the growing awareness about ecofriendly organic farming and biotechnology, coirpith and cowdung natural sources were used to produce vermicompost which showed enhanced growth promoting effect in the crop Cyamopsis tetragonaloba. Initially, neutral detergent fibre, acid detergent fibre, lignin, cellulose and hemicellulose contents were determined in three different grades of coirpith and in cowdung. Vermicompost was prepared using the earthworm Eudrilus eugeniae with 60days maintenance. During composting N, P, K and C contents were estimated in vermicompost as well as in control compost. N, P and K values were highly increased in vermicompost. C:N was dramatically decreased in vermicomposting during the experimental period. Vermicompost enhanced the growth of tested C. tetragonaloba crop. Vermicompost treated plants were taller by 9cm when compared with the control plant. The use of Vermicompost also led to a marked increase in plant biomass

CYTOTOXIC AND ANTIOXIDANT PROPERTIES OF FRACTIONS ISOLATED FROM FERONIA ELEPHANTUM

Objective: The leaves of Feronia elephantum are widely used in folk medicine in India to treat various ailments. In the present communication we report the cytotoxic and antioxidant properties of extracts and fractions of F. elephantum leaves. Methods: The leaves of F. elephantum were extracted with hexane and ethyl acetate and checked for cytotoxic properties against human A549 lung adenocarcinoma cancer cell line. The active extract was subjected to column chromatography and fractions were bio-assayed. The active fraction was subjected to GC-MS analysis. Results: Hexane extract exhibited good cytotoxic activity against A549 lung cancer cell line compared to ethyl acetate extract. Hexane extract showed 77.3% activity at the dose of 500 µg/mL with IC50 (50.6%) value of 125 µg/mL. Hexane extract was also tested at different time intervals of 12h, 24h, 48h and 72 h. The activity gradually increased. The active hexane extract was subjected to column chromatography. Based on thin layer chromatography profiles, similar fractions were combined to give 9 fractions. When the fractions were bio-assayed fraction 5 showed maximum cytotoxic activity. Fraction 5 was tested against A549 lung cancer cell line at different time intervals; the activity gradually increased. Fraction 5 was used to test the antioxidant properties using DPPH analysis. Maximum antioxidant activity was observed at 1000 µg/mL (71.63 ± 0.15). Active fraction 5 was identified using GC-MS. It showed the presence of Estragole (50.82%), trans-anethole (p-propenylanisole, anise camphor) (14.98%) and Caryophyllene (9.22%). Conclusion: The results showed that hexane extract of F. elephantum could be probed further in drug discovery programme