Integrated Soil Fertility and Plant Nutrient Management in Tropical Agro-Ecosystems: A Review

The greatest challenge for tropical agriculture is land degradation and reduction in soil fertility for sustainable crop and livestock production. Associated problems include soil erosion, nutrient mining, competition for biomass for multiple uses, limited application of inorganic fertilizers, and limited capacity of farmers to recognize the decline in soil quality and its consequences on productivity. Integrated soil fertility management (ISFM) is an approach to improve crop yields, while preserving sustainable and long-term soil fertility through the combined judicious use of fertilizers, recycled organic resources, responsive crop varieties, and improved agronomic practices, which minimize nutrient losses and improve the nutrient-use efficiency of crops. Soil fertility and nutrient management studies in Ethiopia under on-station and on-farm conditions showed that the combined application of inorganic and organic fertilizers significantly increased crop yields compared to either alone in tropical agro-ecosystems. Yield benefits were more apparent when fertilizer application was accompanied by crop rotation, green manuring, or crop residue management. The combination of manure and NP fertilizer could increase wheat and faba bean grain yields by 50%–100%, whereas crop rotation with grain legumes could increase cereal grain yields by up to 200%. Although organic residues are key inputs for soil fertility management, about 85% of these residues is used for livestock feed and energy; thus, there is a need for increasing crop biomass. The main incentive for farmers to adopt ISFM practices is economic benefits. The success of ISFM also depends on research and development institutions to provide technical support, technology adoption, information dissemination, and creation of market incentives for farmers in tropical agro-ecosystems

Response of Teff ((Eragrostis tef (zucc.) Trotter) to nitrogen and phosphorus applications on different landscapes in eastern Amhara

The dynamic nature of soil fertility status across different landscapes attracted research attention in Ethiopia and the globe. Teff [Eragrotis tef] is a major staple cereal crop in Ethiopia but yields are low due to inadequate nutrient supply and other constraints. A field study was conducted in 2020 and 2021 in the Habru district of Amhara Region to determine teff yield response to fertilizer-N and -P at hillslope, midslope, and footslope positions with slopes of >15%, 5–15%, and 0–5%, respectively. N and P fertilizer rates were factorially combined in randomized complete block design with three replications in each farmer’s field. A linear mixed modeling framework was used to determine effects on grain yield due to N rate, P rate, slope, study sites, and years. Model fit was examined using Akaike’s Information Criterion and Bayesian Information Criterion. Economic analysis was done with a quadratic response function to determine the economics of fertilizer. Yield response to fertilizer-P was affected by slope but the response to fertilizer-N was not affected. Teff yield increase with fertilizer-N application up to 92 kg ha-1 the economic optimum rate based on the yield response function for nitrogen fertilizer was 85.4 kg ha-1 to obtain maximum profit (86878.8 birr ha-1). Similarly, the optimum phosphorus fertilizer rate at the hill slope was 39.7 kg ha-1 to obtain a maximum profit of (96847.8 birr ha-1). But there was not a profitable response at the midslope and foot slope positions. Therefore, for Habru district and similar agroecologies85.4 kg ha-1 N and 39.7 kg ha-1 P in hillslopes and only 85.4 N kg ha-1 for midslopes and foot slopes are expected to give the most profitable returns to fertilizer applied for tef production

Remediation of acid soils and soil property amelioration via Acacia decurrens-based agroforestry system

Land degradation and the associated soil acidity are critical challenge for crop production in Ethiopian highlands. Since liming is expensive, farmers have developed an alternative agroforestry system by integrating Acacia decurrens into their landscapes. The expansion rate of this system was assessed over the last three decades. The effects of the agroforestry system and charcoal-making kiln sites on soil properties were investigated for over five years compared to the adjacent croplands. Soil samples were collected from A. decurrens plantations, kiln sites, and adjacent croplands at 0–15 and 15–30 cm soil depths. In the last 30 years, the plantation and croplands increased by 8% and 17.5%, respectively, compared to the land-use system in 1993, mainly at the expense of grassland and abandoned land. The main incentive for expansion of A. decurrens plantations was farmers’ income generated from charcoal making. This intervention also improved soil properties with a significantly positive effect on soil pH, soil organic carbon (SOC), cation exchange capacity (CEC), and available Bray phosphorus (Bray-P) compared to the adjacent croplands. Results revealed that the SOC content in year 2 increased significantly (1.3–1.7 times) under A. decurrens plantation compared to adjacent crop fields. Moreover, soil pH increased by one unit on charcoalmaking fields, which was equivalent to application of 4–5 t lime ha-1, while SOC increased by * 10% on kiln sites compared to the control. Charcoal making kiln spots increased available soil phosphorus by 112% compared to the adjacent non-kiln sites. The Bray- P was strongly and significantly (P\0.05) correlated (r = 0.75) with soil pH. We conclude that integrating A. decurrens-based agroforestry practices would improve livelihoods by restoring degraded lands, improving income generation and carbon sequestration

Short term fallow and partitioning effects of green manures on wheat systems in East African highlands

Soil fertility depletion is a major constraint of smallholder farming in sub-Saharan Africa. We tested the aftereffects of green manures, namely vetch (Vicia sativa L.), lupin (Lupinus polyphyllus L.), and lablab (Lablab purpureus L.) incorporated into the soil compared to three fertilizer levels (0/0, 23/0, and 78/20 kg N/P h

Efficacy of Carraguard®-Based Microbicides In Vivo Despite Variable In Vitro Activity

Anti-HIV microbicides are being investigated in clinical trials and understanding how promising strategies work, coincident with demonstrating efficacy in vivo, is central to advancing new generation microbicides. We evaluated Carraguard® and a new generation Carraguard-based formulation containing the non-nucleoside reverse transcriptase inhibitor (NNRTI) MIV-150 (PC-817). Since dendritic cells (DCs) are believed to be important in HIV transmission, the formulations were tested for the ability to limit DC-driven infection in vitro versus vaginal infection of macaques with RT-SHIV (SIVmac239 bearing HIV reverse transcriptase). Carraguard showed limited activity against cell-free and mature DC-driven RT-SHIV infections and, surprisingly, low doses of Carraguard enhanced infection. However, nanomolar amounts of MIV-150 overcame enhancement and blocked DC-transmitted infection. In contrast, Carraguard impeded infection of immature DCs coincident with DC maturation. Despite this variable activity in vitro, Carraguard and PC-817 prevented vaginal transmission of RT-SHIV when applied 30 min prior to challenge. PC-817 appeared no more effective than Carraguard in vivo, due to the limited activity of a single dose of MIV-150 and the dominant barrier effect of Carraguard. However, 3 doses of MIV-150 in placebo gel at and around challenge limited vaginal infection, demonstrating the potential activity of a topically applied NNRTI. These data demonstrate discordant observations when comparing in vitro and in vivo efficacy of Carraguard-based microbicides, highlighting the difficulties in testing putative anti-viral strategies in vitro to predict in vivo activity. This work also underscores the potential of Carraguard-based formulations for the delivery of anti-viral drugs to prevent vaginal HIV infection

Landscape-based nutrient application in wheat and teff mixed farming systems of Ethiopia: farmer and extension agent demand driven approach

Introduction: Adapting fertilizer use is crucial if smallholder agroecosystems are to attain the sustainable development goals of zero hunger and agroecosystem resilience. Poor soil health and nutrient variability characterize the smallholder farming systems. However, the current research at the field scale does not account for nutrient variability across landscape positions, posing significant challenges for targeted nutrient management interventions. The purpose of this research was to create a demand-driven and co-development approach for diagnosing farmer nutrient management practices and determining landscape-specific (hillslope, mid-slope, and foot slope) fertilizer applications for teff and wheat. Method: A landscape segmentation approach was aimed to address gaps in farm-scale nutrient management research as well as the limitations of blanket recommendations to meet local nutrient requirements. This approach incorporates the concept of interconnected socio-technical systems as well as the concepts and procedures of co-development. A smart mobile app was used by extension agents to generate crop-specific decision rules at the landscape scale and forward the specific fertilizer applications to target farmers through SMS messages or print formats. Results and discussion: The findings reveal that farmers apply more fertilizer to hillslopes and less to mid- and foot slopes. However, landscape-specific fertilizer application guided by crop-specific decision rules via mobile applications resulted in much higher yield improvements, 23% and 56% at foot slopes and 21% and 6.5% at mid slopes for wheat and teff, respectively. The optimized net benefit per hectare increase over the current extension recommendation was $176 and$333 at foot slopes and $159 and$64 at mid slopes for wheat and teff (average of $90 and$107 for wheat and teff), respectively. The results of the net benefit-to-cost ratio (BCR) demonstrated that applying landscape-targeted fertilizer resulted in an optimum return on investment ($10.0 net profit per$1.0 investment) while also enhancing nutrient use efficiency across the three landscape positions. Farmers are now cognizant of the need to reduce fertilizer rates on hillslopes while increasing them on parcels at mid- and foot-slope landscapes, which have higher responses and profits. As a result, applying digital advisory to optimize landscape-targeted fertilizer management gives agronomic, economic, and environmental benefits. The outcomes results of the innovation also contribute to overcoming site-specific yield gaps and low nutrient use efficiency, they have the potential to be scaled if complementing innovations and scaling factors are integrated

Dramatic Rise in Plasma Viremia after CD8+ T Cell Depletion in Simian Immunodeficiency Virus–infected Macaques

To determine the role of CD8+ T cells in controlling simian immunodeficiency virus (SIV) replication in vivo, we examined the effect of depleting this cell population using an anti-CD8 monoclonal antibody, OKT8F. There was on average a 99.9% reduction of CD8 cells in peripheral blood in six infected Macaca mulatta treated with OKT8F. The apparent CD8 depletion started 1 h after antibody administration, and low CD8 levels were maintained until day 8. An increase in plasma viremia of one to three orders of magnitude was observed in five of the six macaques. The injection of a control antibody to an infected macaque did not induce a sustained viral load increase, nor did it significantly reduce the number of CD8+ T cells. These results demonstrate that CD8 cells play a crucial role in suppressing SIV replication in vivo

HSV-2 Infection of Dendritic Cells Amplifies a Highly Susceptible HIV-1 Cell Target

Herpes simplex virus type 2 (HSV-2) increases the risk of HIV-1 infection and, although several reports describe the interaction between these two viruses, the exact mechanism for this increased susceptibility remains unclear. Dendritic cells (DCs) at the site of entry of HSV-2 and HIV-1 contribute to viral spread in the mucosa. Specialized DCs present in the gut-associated lymphoid tissues produce retinoic acid (RA), an important immunomodulator, able to influence HIV-1 replication and a key mediator of integrin α4β7 on lymphocytes. α4β7 can be engaged by HIV-1 on the cell-surface and CD4+ T cells expressing high levels of this integrin (α4β7high) are particularly susceptible to HIV-1 infection. Herein we provide in-vivo data in macaques showing an increased percentage of α4β7high CD4+ T cells in rectal mucosa, iliac lymph nodes and blood within 6 days of rectal exposure to live (n = 11), but not UV-treated (n = 8), HSV-2. We found that CD11c+ DCs are a major target of HSV-2 infection in in-vitro exposed PBMCs. We determined that immature monocyte-derived DCs (moDCs) express aldehyde dehydrogenase ALDH1A1, an enzyme essential for RA production, which increases upon HSV-2 infection. Moreover, HSV-2-infected moDCs significantly increase α4β7 expression on CD4+ T lymphocytes and HIV-1 infection in DC-T cell mixtures in a RA-dependent manner. Thus, we propose that HSV-2 modulates its microenviroment, influencing DC function, increasing RA production capability and amplifying a α4β7highCD4+ T cells. These factors may play a role in increasing the susceptibility to HIV-1