A case of cerebral salt wasting syndrome in a patient with central diabetes insipidus and status epilepticus

N/

Effect of feeding extruded flaxseed on milk quality of dairy cows

Aim of the present study was to evaluate the effect of feeding dairy cows with extruded flaxseed (EF) on milk quality. After a 7-d adaptation period, 40 Holstein dairy cows in midlactation were divided in two experimental groups, based on parity, milk yield and composition, and days in milk. Animals were fed for 28 d a standard total mixed ration containing or not (control) 1.8 kg/cow/d of a supplement based on extruded flaxseed (EF). Milk yield was recorded daily and individual milk samples were collected weekly for analysis. Milk yield was not affected by treatment and averaged 26.2 kg/d throughout the study. Feeding EF reduced milk fat (3.95 vs. 4.24%; P=0.053) and tended to reduce milk protein (3.32 vs. 3.50%; P=0.104). The milk from cows fed EF contained more (P<0.01) stearic (12.0 vs. 7.8 mg/100 mg of fat), oleic (21.2 vs. 18.0 mg/100 mg of fat), \u3b1-linolenic (0.85 vs. 0.41 mg/100 mg of fat) and vaccenic (1.43 vs. 0.62 mg/100 mg of fat) acids than control and also more c9,t11 CLA (0.91 vs. 0.59 mg/100 mg of fat). Conversely, compared with control, feeding EF reduced milk concentrations of palmitic acid (26.5 vs. 33.4 mg/100 mg of fat; P<0.01). The present results show that feeding EF to dairy cows is an efficient strategy to enrich milk in beneficial fatty acids such as vaccenic acid, CLA and omega-3 fatty acids. Nevertheless, the milk fat reducing effect of flaxseed as well as other sources of polyunsaturated fatty acids should be taken into account

Calving difficulty influences rumination time and inflammatory profile in Holstein dairy cows

Difficult calving may adversely affect dairy cow health and performance. Maternal:fetal disproportion is a major cause of dystocia. Therefore, the main objective of this study was to assess the effects of dam:calf body weight ratio (D:C) on calving difficulty, rumination time, lying time, and inflammatory profile in 25 Holstein dairy cows. Using automatic monitoring systems, we monitored behavior and production in 9 primiparous and 16 pluriparous cows between dry-off and 30 d in milk. During the same period, we collected blood samples to monitor metabolism and inflammatory profile of these cows. Calvings were video recorded to assess calving difficulty and observe the duration of the expulsive stage. After parturition, the cows were separated into 3 classes according to their D:C: easy (E; D:C &gt;17), medium (M; 14 &lt; D:C &lt;17), and difficult (D; D:C &lt;14). The cows in class D showed relatively longer labor durations (108 min vs. 54 and 51 min for classes D, M, and E, respectively) and higher calving assistance rates (50% vs. 0 and 11% of calvings for classes D, M, and E, respectively) than those in the other 2 classes. Compared with the cows in classes M and E, those in class D exhibited shorter rumination times on the day of calving (176 min/d vs. 288 and 354 min/d for classes D, M, and E, respectively) and during the first week of lactation (312 min/d vs. 339 and 434 min/d for classes D, M, and E, respectively) and maintained lower rumination values until 30 DIM (399 min/d vs. 451 and 499 min/d for classes D, M, and E, respectively). Primiparous class D cows had shorter resting times during the first week after calving compared with those in class M (8 vs. 11 h/d for classes D and M, respectively). Interclass differences were found in terms of the levels of inflammation markers such as acute-phase proteins (ceruloplasmin, albumin, retinol, and paraoxonase). Moreover, cows in class D had lower plasma levels of fructosamine and creatinine after calving. Low D:C reduced postcalving rumination time and increased inflammation grade, suggesting a lower welfare of these animals at the onset of lactation. The D:C might serve as a useful index for the identification of cows at relatively higher risk of metabolic and inflammatory disease, thus helping farmers and veterinarians improve the welfare and health of these cows

Overstocking dairy cows during the dry period affects dehydroepiandrosterone and cortisol secretion

Stressful situations trigger several changes such as the secretion of cortisol and dehydroepiandrosterone (DHEA) from the adrenal cortex, in response to ACTH. The aim of this study was to verify whether overstocking during the dry period (from 21&nbsp;\ub1&nbsp;3 d to the expected calving until calving) affects DHEA and cortisol secretion and behavior in Holstein Friesian cows. Twenty-eight cows were randomly divided into 2 groups (14 animals each), balanced for the number of lactations, body condition score, and expected date of calving. Cows in the far-off phase of the dry period (from 60 to 21 d before the expected calving date) were housed together in a bedded pack. Then, animals from 21&nbsp;\ub1&nbsp;3 d before the expected calving until calving were housed in pens with the same size but under different crowding conditions due to the introduction of heifers (interference animals) into the pen. The control condition (CTR) had 2 animals per pen with 12.0&nbsp;m2 each, whereas the overstocked condition (OS) had 3 interference animals in the same pen with 4.8&nbsp;m2 for each animal. On d 1230&nbsp;\ub1&nbsp;3, 1221&nbsp;\ub1&nbsp;3, 1215&nbsp;\ub1&nbsp;3, 1210&nbsp;\ub1&nbsp;3, and 125&nbsp;\ub1&nbsp;3 before and 10, 20, and 30 after calving, blood samples were collected from each cow for the determination of plasma DHEA and cortisol concentrations by RIA. Rumination time (min/d), activity (steps/h), lying time (min/d), and lying bouts (bouts/d) were individually recorded daily. In both groups, DHEA increased before calving and the concentration declined rapidly after parturition. Overstocking significantly increased DHEA concentration compared with the CTR group at d 1210 (1.79&nbsp;\ub1&nbsp;0.09 vs. 1.24&nbsp;\ub1&nbsp;0.14 pmol/mL), whereas an increase of cortisol was observed at d 1215 (3.64&nbsp;\ub1&nbsp;0.52 vs. 1.64&nbsp;\ub1&nbsp;0.46&nbsp;ng/mL). The OS group showed significantly higher activity (steps/h) compared with the CTR group. Daily lying bouts tended to be higher for the OS group compared with CTR group in the first week of treatment. The overall results of this study documented that overstocking during the dry period was associated with a short-term changes in DHEA and cortisol but these hormonal modifications did not influence cow behavior

Survey on antimicrobial residues in raw milk and antimicrobial use in dairy farms in the Emilia-Romagna Region, Italy

This survey investigated the antimicrobials most commonly used in dairy herds and t antimicrobial residues most frequently detected in milk to evaluate the suitability of rapid screening tests to determine antimicrobial residues in milk. The investigation was carried out in 45 dairy herds consulting the farm administration records and in a national dairy industry collecting milk from almost all the dairy farms studied. Data were recorded on: i) treatments with drugs containing antimicrobials during the 12 months prior to the visit; ii) antimicrobial active substances present in the drugs; iii) data from routine controls to detect antimicrobial residues (52,771 samples). The antimicrobial classes most commonly used were penicillins, cephalosporins, fluoroquinolones, macrolides, sulphonamides, tetracyclines, aminoglycosides and lyncosamides; the most frequently used antimicrobial not belonging to any of the previous groups was riphaximin. Sixty-four samples collected from milk trucks yielded antimicrobial residues exceeding the detection limit of the screening test used: sulphonamide residues were the most prevalent (3.4%), followed by tetracycline (0.3%) and penicillins and cephalosporins (0.03%). The antimicrobial classes most commonly used on dairy farms are the same as the residues most frequently detected in milk. The association of several commercially available rapid test kits proved satisfactory for determination of the veterinary antimicrobial drugs most used on dairy farms but at least five kits are required. Therefore knowledge of the most frequently used veterinary drugs and periodic monitoring are required for the dairy industry to develop a targeted and effective control pla

Endoscopic Transorbital Approach for Spheno-Orbital Tumors: Case Series and Systematic Review of Literature

Background: Transorbital neuroendoscopic surgery (TONES) comprises a group of approaches with indications expanding from orbital tumors to more complex skull base lesions. We analyzed the role of the endoscopic transorbital approach (eTOA) for spheno-orbital tumors, reporting the results of our clinical series and of a systematic review of the literature. Materials and Methods: All patients operated on from 2016 to 2022 at our institution for a spheno-orbital tumor through an eTOA were included in a clinical series, and a systematic review of the literature was performed. Results: Our series consisted of 22 patients (16 females, mean age 57 ± 13 years). Gross tumor removal was achieved in 8 patients (36.4%) after the eTOA and in 11 (50.0%) after a multistaged strategy combining the eTOA with the endoscopic endonasal approach. Complications included 1 chronic subdural hematoma and 1 permanent extrinsic ocular muscle deficit. Patients were discharged after 2.4 ± 1.3 days. The most common histotype was meningioma (86.4%). Proptosis improved in all cases, visual deficit in 66.6%, and diplopia in 76.9%. These results were confirmed by the review of the 127 cases reported in the literature. Conclusions: Despite its recent introduction, a significant number of spheno-orbital lesions treated with an eTOA are being reported. Its main advantages are favorable patient outcome and optimal cosmetic results, with minimal morbidity and quick recovery. This approach can be combined with other surgical routes or adjuvant therapies for complex tumors. However, it is a technically demanding procedure, requiring specific skills in endoscopic surgery, that should be reserved to dedicated centers

Erectile dysfunction is common among men with acromegaly and is associated with morbidities related to the disease

Background: The prevalence of erectile dysfunction (ED) and its correlates in men with acromegaly has never been investigated. Aim: To evaluate sexual function in men with acromegaly. Methods: Multicenter-based, retrospective analysis of a non-selected series of 57 acromegalic subjects (mean age: 52.7 14.2 years) was performed. Acromegalic subjects reporting ED (n = 24) were compared with matched ED- patients without acromegaly or pituitary disease (con- trols), selected from a cohort of more than 4000 subjects enrolled in the Flrence Sexual Medicine and Andrology Unit. Patients were interviewed using SIEDY structured interview, a 13-item tool for the assessment of ED-related morbidities. Several clinical and biochemical parameters were taken. Penile colour-Doppler ultrasound (PCDU) was performed in a subgroup of 37 acromegalic subjects. Results: ED was reported by 42.1% of acromegalic sub- jects. After adjusting for age and testosterone, acromegalic subjects with ED had a higher prevalence of hypertension, and more often reported an impairment of sleep-related erections and a longer smoking habit. Accordingly, acro- megaly-associated ED was characterized by a higher organic component and worse PCDU parameters. No rela- tionship between ED and testosterone levels or other acro- megaly-related parameters was found. However, acromegalic subjects with severe ED reported a longer dis- ease duration. In a case-control analysis, comparing acromegalic subjects with ED-matched-controls free from acromegaly (1:5 ratio), acromegalic men had a worse ED problem and a higher organic component of ED, as derived from SIEDY score. In line with these data, acrome- galic patients with ED had a higher prevalence of major adverse cardiovascular events (MACE) history at enrol- ment and lower PCDU parameters. Conclusions: Subjects with complicated acromegaly are at an increased risk of developing ED, especially those with cardiovascular morbidities. Our data suggest including a sexual function evaluation in routine acromegaly follow- up

Ectopic TSH-secreting pituitary adenoma of the vomerosphenoidal junction

OBJECTIVE: We describe an unusual case of ectopic TSH-secreting pituitary adenoma arising from the vomerosphenoidal junction. CLINICAL PRESENTATION: A 52-Year-old man with a long-standing history of hyperthyroidism was referred to the University Hospital in September 2001 because of increasingly disabling symptoms of nasal obstruction. For the past 18 Years the patient had complained of palpitations, hypertension, weight loss, and nervousness. A presumptive diagnosis of Graves' disease was made. Treatment with methimazole was begun, but the patient was lost to follow-up. On admission, physical examination revealed signs of hyperthyroidism and a large diffuse goiter. Tests of thyroid function showed inappropriate secretion of TSH with hyperthyroidism. Both a TSH-secreting pituitary adenoma and resistance to thyroid hormone could be taken into account. There was no evidence of pituitary tumour by magnetic resonance imaging (MRI), but a large space-occupying lesion involving the nasal cavity and the nasopharynx was incidentally discovered. INTERVENTATION AND TECHNIQUE: Using an endoscopic endonasal approach, the tumour was removed en bloc together with the sphenoid floor, sphenoid rostrum, bony septum, and part of the soft palate mucosa. Histological features and immunophenotype were those of a TSH-secreting tumour. CONCLUSION: Although exceedingly rare, ectopic TSH-secreting pituitary tumour should be borne in mind in cases of inappropriate secretion of TSH with hyperthyroidism and no evidence of pituitary tumour by computed tomography and/or MRI when a mass located along the migration path of the Rathke's pouch is demonstrated by radiological examination. To our knowledge, this is only the second reported case in the literature

Four domains for concurrency

AbstractWe give four domains for concurrency in a uniform way by means of domain equations. The domains are intended for modelling the four possible combinations of linear time versus branching time, and of interleaving versus noninterleaving concurrency. We use the linear time, noninterleaved domain to give operational and denotational semantics for a simple concurrent language with recursion, and prove that O = D