Role of the Schizosaccharomyces pombe F-box DNA helicase in processing recombination intermediates.

In an effort to identify novel genes involved in recombination repair, we isolated fission yeast Schizosaccharomyces pombe mutants sensitive to methyl methanesulfonate (MMS) and a synthetic lethal with rad2. A gene that complements such mutations was isolated from the S. pombe genomic library, and subsequent analysis identified it as the fbh1 gene encoding the F-box DNA helicase, which is conserved in mammals but not conserved in Saccharomyces cerevisiae. An fbh1 deletion mutant is moderately sensitive to UV, MMS, and ¿ rays. The rhp51 (RAD51 ortholog) mutation is epistatic to fbh1. fbh1 is essential for viability in stationary-phase cells and in the absence of either Srs2 or Rqh1 DNA helicase. In each case, lethality is suppressed by deletion of the recombination gene rhp57. These results suggested that fbh1 acts downstream of rhp51 and rhp57. Following UV irradiation or entry into the stationary phase, nuclear chromosomal domains of the fbh1¿ mutant shrank, and accumulation of some recombination intermediates was suggested by pulsed-field gel electrophoresis. Focus formation of Fbh1 protein was induced by treatment that damages DNA. Thus, the F-box DNA helicase appears to process toxic recombination intermediates, the formation of which is dependent on the function of Rhp51

Natural replacement of vertically inherited lux‐rib genes of Photobacterium aquimaris by horizontally acquired homologues

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/92440/1/emi4355_sm_FigS1.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/92440/2/emi4355_sm_TableS1.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/92440/3/emi4355.pd

Free Surface Flow in Vertical Taylor-Couette System

Vertical Taylor-Couette flow with a free surface at its top has been examined by numerical and experimental approaches. Being compared with the ideal case with infinite length cylinders, this system has effects of the finite lengths, the gravitational acceleration and the surface tension. The inner cylinder rotates and the outer cylinder and the bottom wall are stationary. The numerical approach uses the VOF method to model the free surface. In the experiment, the flow patterns are observed from the top and the meridional views, and the displacement of the free surface is measured. The flow modes are classified by the number of vortices appearing in the meridional plane and the radial flow directions at the top and the bottom. The power spectra of the displacement of the free surface and the bulk energy are numerically evaluated and the bulk energy tends to give favorable agreement with the spectra of the displacement obtained experimentally. The transition from the secondary mode flow appearing at higher Reynolds number to the primary mode flow at lower Reynolds number is examined and comparison between the numerical and experimental results are made

Royal Jelly Facilitates Restoration of the Cognitive Ability in Trimethyltin-Intoxicated Mice

Trimethyltin (TMT) is a toxic organotin compound that induces acute neuronal death selectively in the hippocampal dentate gyrus (DG) followed by cognition impairment; however the TMT-injured hippocampal DG itself is reported to regenerate the neuronal cell layer through rapid enhancement of neurogenesis. Neural stem/progenitor cells (NS/NPCs) are present in the adult hippocampal DG, and generate neurons that can function for the cognition ability. Therefore, we investigated whether royal jelly (RJ) stimulates the regenerating processes of the TMT-injured hippocampal DG, and found that orally administered RJ significantly increased the number of DG granule cells and simultaneously improved the cognitive impairment. Furthermore, we have already shown that RJ facilitates neurogenesis of cultured NS/NPCs. These present results, taken together with previous observations, suggest that the orally administered RJ may be a promising avenue for ameliorating neuronal function by regenerating hippocampal granule cells that function in the cognition process