コレステロール食投与ラット胆汁酸・胆汁脂質に対するパンテチン，コエンザイムA前駆体の効果

The effect of pantethine on bile acids and lipids was studied by the washout technique in Wistar female rats fed for four weeks with a 1% cholesterol diet supplemented with 0.5% cholic acid, either containing or not containing 1% pantethine. Pantethine caused a slight increase in the biliary cholesterol concentration with a significant decrease in the serum cholesterol, β-lipoprotein and triglyceride levels. An increase in the pool size, secretion rate and enterohepatic circulation rate of primary bile acids was at the same time observed in the animals. The production of lithogenic bile was, therefore, inhibited in rats fed with a cholesterol diet treated with pantethine. An analysis of conjugated bile acids revealed a decrease in the glycine/taurine ratio of the whole bile acids as detected by high performance liquid chromatography as a result of pantethine administration

ガスクロマトグラフィー法による肝胆道疾患の血清胆汁酸測定について : 空腹時血清胆汁酸および内因性胆汁酸負荷テスト

The methods and results of a gas-liquid chromatographic analysis of bile acids in serum are presented. The analysis of bile acids in serum involves enzymatic hydrolysis (cholylglycine hydrolase), preparation of propionated methyl ester derivatives of bile acid and gas chromatographic procedure with 2.5 % OV-1. Adequate separation of the individual bile acids, (cholic, chenodeoxycholic and deoxycholic acid) was achieved with vitamine E caprylate as an internal standard. A detector response was linear and recovery of radioactive taurocholic acid and non-radioactive vitamine E caprylate added to the serum was 82.10±6.86 and 80.96±1.62% respectively. The serum fasting bile acid concentrations of normal controls were 3.17±2.34 for total bile acids, 1.17±1.25 for cholic acid, 1.34±2.11 for chenodeoxycholic acid and 1.29±0.95 μg/ml for deoxycholic acid. The differences in the serum total bile acid levels, magnitude of the increase in the serum concentration between cholic and chenodeoxycholic acid and serum concentration level of deoxycholic acid which were all characterized in various hepatobiliary diseases seemed to be useful for the diagnosis and differential diagnosis of hepatobiliary diseases. However, these serum bile acid concentrations were frequently observed overlapped in some of the individuals among hepatobiliary diseases. An endogenous bile add tolerance test with 2 μg/kg caerulein injection demonstrated more distinction in serum bile acid levels between normal and chronic active hepatitis and between chronic active hepatitis and liver cirrhosis than indicated by a fasting total bile acid level alone. Percent chenodeoxycholic acid increased more than any other individual bile acids during the endogenous bile acid tolerance test suggesting the most important role of chenodeoxycholic acid