A Reappraisal of the Tokyo District Court\u27s Decision on the Shimoda Case (1)

この研究は昭和四九年度文部省科学研究費一般研究D「広島・長崎原爆の戦争法上の評価」による研究成果の一部である

Réflexions sur l\u27Order Mondial au XXIème Siècle

創立百二十周年記念特

広島・長崎原爆の戦争法上の評価

金沢大学法文学部研究課題/領域番号:X00095----962003, 研究期間(年度):1974出典：「広島・長崎原爆の戦争法上の評価」研究成果報告書　課題番号：X00095----962003（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） （https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-X00095----962003/）を加工して作

Expression of a Cross-reactive Antigen on the Surface of Human Carcinomas Overproducing Epidermal Growth Factor Receptors Shared with RSV induced Tumors

Analysis based on the complement-dependent cytotoxicity (CDC) assays using syngeneic antiserum against a Rous sarcoma virus (RSV)-induced mouse tumor (CSA1M) showed that a cross-reactive antigen with a common tumor-associated cell surface antigen (TASA) of RSV-induced mouse tumors was shared with two human tumors A431 and MDA-468 overexpressing epidermal growth factor receptors (EGFRs). The TASA, however, was not expressed on four human choriocarcinomas, a human lung cancer A2182, and human embryo fibroblasts HEF. Immunofluorescent studies demonstrated that A431 did not express a src gene product detected by rabbit anti-pp60V-src serum. Two variant clones derived from A431 reducing number of EGFRs (cl-15 and cl-16) had almost the same growth rate and expression of transferrin receptor (Tf-R) in comparison with parental A431 cells. These clones, however, decreased the expression of the TASA. Furthermore, CDC assays and enzyme-linked immunosorbent assays (ELISA) revealed that A431 reduced the expression of the TASA by pretreatment of EGF, but not with insulin. A truncated form corresponding to extracellular domain of the EGFR blocked the cytotoxicity of anti-CSA1M serum to A431 and RSV-induced tumor cells. All these findings indicate a close association between a cross-reactive antigen with the TASA of RSV-induced tumors and the EGFR expression