A three-dimensional microfluidic tumor cell migration assay to screen the effect of anti-migratory drugs and interstitial flow

Most anti-cancer drug screening assays are currently performed in two dimensions, on flat, rigid surfaces. However, there are increasing indications that three-dimensional (3D) platforms provide a more realistic setting to investigate accurate morphology, growth, and sensitivity of tumor cells to chemical factors. Moreover, interstitial flow plays a pivotal role in tumor growth. Here, we present a microfluidic 3D platform to investigate behaviors of tumor cells in flow conditions with anti-migratory compounds. Our results show that interstitial flow and its direction have significant impact on migration and growth of hepatocellular carcinoma cell lines such as HepG2 and HLE. In particular, HepG2/HLE cells tend to migrate against interstitial flow, and their growth increases in interstitial flow conditions regardless of the flow direction. Furthermore, this migratory activity of HepG2 cells is enhanced when they are co-cultured with human umbilical vein endothelial cells. We also found that migration activity of HepG2 cells attenuates under hypoxic conditions. In addition, the effect of Artemisinin, an anti-migratory compound, on HepG2 cells was quantitatively analyzed. The microfluidic 3D platform described here is useful to investigate more accurately the effect of anti-migratory drugs on tumor cells and the critical influence of interstitial flow than 2D culture models.Japan Society for the Promotion of Science (22680037)Japan Society for the Promotion of Science (G2212)National Cancer Institute (U.S.) (R21CA140096)Japan. Ministry of Education, Culture, Sports, Science and Technology (2009-00631)Japan. Ministry of Education, Culture, Sports, Science and Technology (2012-0009565)Korea (South). Ministry of Education & Human Resources Development (MOEHRD)Korea (South). Ministry of Education & Human Resources Development (MOEHRD) (20124010203250

ソウカン イドウ ショクバイ オ モチイタ ニトロアルカン ノ フホウワ カルボニル カゴウブツ エ ノ フセイ キョウヤク フカ ハンノウ ノ カイハツ

京都大学0048新制・課程博士博士(理学)甲第12137号理博第3031号新制||理||1452(附属図書館)23973UT51-2006-J131京都大学大学院理学研究科化学専攻(主査)教授 丸岡 啓二, 教授 林 民生, 教授 大須賀 篤弘学位規則第4条第1項該当Doctor of ScienceKyoto UniversityDA

Detection of Edge-Conducting Channels in Quantum Hall Systems Using a Single-Electron Transistor

We report detection of the edge channel of a two-dimensional electron gas (2DEG) in quantum Hall regime, through the measurement of the chemical potential variation and the compressibility of a 2DEG using a single-electron transistor (SET) on top of the 2DEG. The measurements at higher frequencies enabled us to detect the incompressible electronic states of the fractional quantum Hall effect (FQHE), the results of which indicate a method for resolving the edge channel of FQHE spatially

Combined Source and Channel Coding System Using Different Importance of Multi-Media Information

In this paper, we propose a combined source and channel coding system for multimedia information sources, which outputs different kinds of information on a frame by frame basis. We use a source encoder which has several probability tables for each medium and a channel encoder which has several convolutional codes for unequal error protection. Computer simulation shows the performance of our system. I. Introduction Recently, the importance of multimedia communication, which has information sources like image, voice and text, is increasing. However for multimedia communication, we need a high bit rate channel. It is hard to send raw data without compression. Also, intelligent communication systems, which can efficiently correct channel errors by taking into account the information contents and achieve highly reliable communication for human receivers, are popular. In this field, intelligent error controlling schemes are considered to protect information effectively from channel errors ..

A Case of Tubulointerstitial Nephritis and Uveitis (TINU) Syndrome with High ASLO Titer

Tubulointerstitial nephritis and uveitis (TINU) syndrome is a rare autoimmune disease and the pathogenesis is still unknown. We report a case of TINU syndrome with high ASLO titer. Uveitis improved and urine β 2 -MG normalized with low dose systemic predonisolone and cyclosporin A. The high ASLO titer in early phase suggested that streptococcal infection might have triggered TINU syndrome. Lymphocyte phenotypes normalized after treatment with low dose systemic predonisolone and cyclosporin A