3 research outputs found
Regulation of stanniocalcin secretion by calcium and PTHrP in Gilthead Seabream (Sparus aurata)
Calcium balance is of paramount importance for vertebrates. In fish, the endocrine modulators of calcium homeostasis include the stanniocalcin (STC), and some members of the parathyroid
hormone (PTH) family, such as the PTH-related protein (PTHrP), acting as antagonists. STC is
ubiquitously expressed in higher vertebrates. In turn, bony fish exhibit specific STC-producing
glands named the corpuscles of Stannius (CS). Previous studies pointed to a calcium-sensing receptor
(CaSR) involvement in the secretion of STC, but little is known of the involvement of other putative
regulators. The CS provides a unique model to deepen the study of STC secretion. We developed an
ex vivo assay to culture CS of fish and a competitive ELISA method to measure STC concentrations.
As expected, STC released from the CS responds to CaSR stimulation by calcium, calcimimetics, and
calcilytic drugs. Moreover, we uncover the presence (by PCR) of two PTHrP receptors in the CS, e.g.,
PTH1R and PTH3R. Thus, ex vivo incubations revealed a dose-response inhibition of STC secretion
in response to PTHrP at basal Ca2+ concentrations. This inhibition is achieved through specific and
reversible second messenger pathways (transmembrane adenylyl cyclases and phospholipase C),
as the use of specific inhibitors highlights. Together, these results provide evidence for endocrine
modulation between two antagonist hormones, STC and PTHrP.info:eu-repo/semantics/publishedVersio
PACAP system evolution and its role in melanophore function in teleost fish skin
Pituitary adenylate cyclase-activating polypeptide (PACAP) administered to tilapia melanophores ex-vivo causes significant pigment aggregation and this is a newly identified function for this peptide in fish. The G-protein coupled receptors (GPCRs), adcyap1r1a (encoding Pac1a) and vipr2a (encoding Vpac2a), are the only receptors in melanophores with appreciable levels of expression and are significantly (p < 0.05) down-regulated in the absence of light. Vpac2a is activated exclusively by peptide histidine isoleucine (PHI), which suggests that Pac1a mediates the melanin aggregating effect of PACAP on melanophores. Paradoxically activation of Pac1a with PACAP caused a rise in cAMP, which in fish melanophores is associated with melanin dispersion. We hypothesise that the duplicate adcyap1ra and vipr2a genes in teleosts have acquired a specific role in skin and that the melanin aggregating effect of PACAP results from the interaction of Pac1a with Ramp that attenuates cAMP-dependent PKA activity and favours the Ca(2+)/Calmodulin dependent pathway.info:eu-repo/semantics/publishedVersio
High rates of intestinal bicarbonate secretion in seawater tilapia (Oreochromis mossambicus)
Osmoregulation in fish is a complex process that requires the orchestrated cooperation of many tissues. In fish facing hyperosmotic environments, the intestinal absorption of some monovalent ions and the secretion of bicarbonate are key processes to favor water absorption. In the present study, we showed that bicarbonate levels in the intestinal fluid are several fold higher in seawater than in freshwater acclimated tilapia (Oreochromis mossambicus). In addition, we analyzed gene expression of the main molecular mechanisms involved in HCO3- movements i.e. slc26a6, slc26a3, slc4a4 and v-type H-ATPase sub C in the intestine of tilapia acclimated to both seawater and freshwater. Our results show an anterior/posterior functional regionalization of the intestine in tilapia in terms of expression patterns, which is affected by environmental salinity mostly in the anterior and mid intestine. Analysis of bicarbonate secretion using pH-Stat in tissues mounted in Ussing chambers reveals high rates of bicarbonate secretion in tilapia acclimated to seawater from anterior intestine to rectum ranging between similar to 900 and similar to 1700 nmol HCO3- cm(-2) h(-1). However, a relationship between the expression of slc26a6, slc26a3, slc4a4 and the rate of bicarbonate secretion seems to be compromised in the rectum. In this region, the low expression of the bicarbonate transporters could not explain the high bicarbonate secretion rates here described. However, we postulate that the elevated v-type H-ATPase mRNA expression in the rectum could be involved in this process. (C) 2017 Elsevier Inc. All rights reserved.University of Cadiz, SpainFCT, Portugal [SFRH/BD/113363/2015]Portuguese Foundation for Science and Technology (Ministry of Science and Higher Education, Portugal) [PTDC/MAR-BIO/3034/2014]Portuguese Foundation for Science and Technology (European Social Funds) [PTDC/MAR-BIO/3034/2014]national funds from the Portuguese Foundation for Science and Technology (FCT) [UID/Multi/04326/2013