Wind effects on a long span steel roof structure: numerical simulation and equivalent static wind loads

A wind tunnel test is conducted in this study on the scaled model of the Guangzhou International Sports Arena (GISA). Simultaneous pressure measurements are conducted in a simulated suburban boundary layer flow field. A numerical simulation approach using Fuzzy Neural Networks (FNNs) is developed for the predictions of wind-induced pressure time series at roof locations which are not covered in the wind tunnel measurements. On the other hand, the wind-induced response of the roof are presented and discussed, which are directly calculated by the Complete Quadratic Combination (CQC) approach. Furthermore, the correlations between the background and resonant response components are discussed in detail, and the results show that neglecting the correlations between the two components would result in considerable error in the response estimation. Finally, the Equivalent Static Wind Load (ESWL) approach is used to estimate the wind-induced responses of the roof, which are compared with those obtained from the CQC approach to examine the effectiveness of the proposed ESWL approach in the design and analysis of large-span roof structures. It is shown through the example that the FNN and ESWL approaches can successfully predict the wind-induced pressures and responses respectively

Demonstration of Deutsch's Algorithm on a Stable Linear-Optical Quantum Computer

We report an experimental demonstration of quantum Deutsch's algorithm by using linear-optical system. By employing photon's polarization and spatial modes, we implement all balanced and constant functions for quantum computer. The experimental system is very stable and the experimental data are excellent in accordance with the theoretical results.Comment: 7 pages, 4 figure

Hybrid quantum device based on NV centers in diamond nanomechanical resonators plus superconducting waveguide cavities

We propose and analyze a hybrid device by integrating a microscale diamond beam with a single built-in nitrogen-vacancy (NV) center spin to a superconducting coplanar waveguide (CPW) cavity. We find that under an ac electric field the quantized motion of the diamond beam can strongly couple to the single cavity photons via dielectric interaction. Together with the strong spin-motion interaction via a large magnetic field gradient, it provides a hybrid quantum device where the dia- mond resonator can strongly couple both to the single microwave cavity photons and to the single NV center spin. This enables coherent information transfer and effective coupling between the NV spin and the CPW cavity via mechanically dark polaritons. This hybrid spin-electromechanical de- vice, with tunable couplings by external fields, offers a realistic platform for implementing quantum information with single NV spins, diamond mechanical resonators, and single microwave photons.Comment: Accepted by Phys. Rev. Applie

miR-15a and miR-16-1 inhibit the proliferation of leukemic cells by down-regulating WT1 protein level

<p>Abstract</p> <p>Background</p> <p>miR-15a and miR-16-1(miR-15a/16-1) have been implicated as tumor suppressors in chronic lymphocytic leukemia, multiple myeloma, and acute myeloid leukemic cells. However the mechanism of inhibiting the proliferation of leukemic cells is poorly understood.</p> <p>Methods</p> <p>K562 and HL-60 cells were transfected with pRS-15/16 or pRS-E, cell growth were measured by CCK-8 assay and direct cell count. Meanwhile WT1 protein and mRNA level were measured by Western blotting and quantitative real-time PCR.</p> <p>Results</p> <p>In this study we found that over-expression of miR-15a/16-1 significantly inhibited K562 and HL-60 cells proliferation. Enforced expression of miR-15a/16-1 in K562 and HL-60 cells significantly reduced the protein level of WT1 but not affected the mRNA level. However enforced expression of miR-15a/16-1 can not reduce the activity of a luciferase reporter carrying the 3'-untranslated region(3'UTR) of WT1. Silencing of WT1 by specific siRNA suppressed leukemic cells proliferation resembling that of miR-15a/16-1 over-expression. Anti-miR-15a/16-1 oligonucleotides (AMO) reversed the expression of WT1 in K562 and HL-60 cells. Finally, we found a significant inverse correlation between miR-15a or miR-16-1 expression and WT1 protein levels in primary acute myeloid leukemia (AML) blasts and normal controls.</p> <p>Conclusions</p> <p>These data suggest that miR-15a/16-1 may function as a tumor suppressor to regulate leukemic cell proliferation potentially by down-regulating the WT1 oncogene. However WT1 is not directly targeted by miR-15a/16-1 through miRNA-mRNA base pairing, therefore more study are required to understand the mechanism by which miR-15a/16-1 downregulate WT1.</p