Optical alignment and spinning of laser-trapped microscopic particles

Light-induced rotation of absorbing microscopic particles by transfer of angular momentum from light to the material raises the possibility of optically driven micromachines. The phenomenon has been observed using elliptically polarized laser beams or beams with helical phase structure. But it is difficult to develop high power in such experiments because of overheating and unwanted axial forces, limiting the achievable rotation rates to a few hertz. This problem can in principle be overcome by using transparent particles, transferring angular momentum by a mechanism first observed by Beth in 1936, when he reported a tiny torque developed in a quartz waveplate due to the change in polarization of transmitted light. Here we show that an optical torque can be induced on microscopic birefringent particles of calcite held by optical tweezers. Depending on the polarization of the incident beam, the particles either become aligned with the plane of polarization (and thus can be rotated through specified angles) or spin with constant rotation frequency. Because these microscopic particles are transparent, they can be held in three-dimensional optical traps at very high power without heating. We have observed rotation rates in excess of 350 Hz.Comment: 4 pages, 4 figure

Optical microrheology using rotating laser-trapped particles

We demonstrate an optical system that can apply and accurately measure the torque exerted by the trapping beam on a rotating birefringent probe particle. This allows the viscosity and surface effects within liquid media to be measured quantitatively on a micron-size scale using a trapped rotating spherical probe particle. We use the system to measure the viscosity inside a prototype cellular structure.Comment: 5 pages, 4 figures. v2: bibliographic details, minor text correction

Indicators of implicit and explicit social anxiety influence threat-related interpretive bias as a function of working memory capacity

Interpretive biases play a crucial role in anxiety disorders. The aim of the current study was to examine factors that determine the relative strength of threat-related interpretive biases that are characteristic of individuals high in social anxiety. Different (dual process) models argue that both implicit and explicit processes determine information processing biases and behavior, and that their impact is moderated by the availability of executive resources such as working memory capacity (WMC). Based on these models, we expected indicators of implicit social anxiety to predict threat-related interpretive bias in individuals low, but not high in WMC. Indicators of explicit social anxiety should predict threat-related interpretive bias in individuals high, but not low in WMC. As expected, WMC moderated the impact of implicit social anxiety on threat-related interpretive bias, although the simple slope for individuals low in WMC was not statistically significant. The hypotheses regarding explicit social anxiety (with fear of negative evaluation used as an indicator) were fully supported. The clinical implications of these findings are discussed

Optical application and measurement of torque on microparticles of isotropic nonabsorbing material

We show how it is possible to controllably rotate or align microscopic particles of isotropic nonabsorbing material in a TEM00 Gaussian beam trap, with simultaneous measurement of the applied torque using purely optical means. This is a simple and general method of rotation, requiring only that the particle is elongated along one direction. Thus, this method can be used to rotate or align a wide range of naturally occurring particles. The ability to measure the applied torque enables the use of this method as a quantitative tool--the rotational equivalent of optical tweezers based force measurement. As well as being of particular value for the rotation of biological specimens, this method is also suitable for the development of optically-driven micromachines.Comment: 8 pages, 6 figure

Grid Workflow Modelling Using Grid-Specific BPEL Extensions

This paper discusses problems of Grid service composition using BPEL4WS. In particular, difficulties concerning the invocation of WSRF-based services are elucidated. A solution to this problem is presented by extending the BPEL specification, and an implementation based on the ActiveBPEL workflow enactment engine is described

Theory of Optical Tweezers

We derive a partial-wave (Mie) expansion of the axial force exerted on a transparent sphere by a laser beam focused through a high numerical aperture objective. The results hold throughout the range of interest for practical applications. The ray optics limit is shown to follow from the Mie expansion by size averaging. Numerical plots show large deviations from ray optics near the focal region and oscillatory behavior (explained in terms of a simple interferometer picture) of the force as a function of the size parameter. Available experimental data favor the present model over previous ones.Comment: 4 pages, 3 figure

Optical angular momentum transfer to trapped absorbing particles

Particle rotation resulting from the absorption of light carrying angular momentum has been measured. When absorbing CuO particles (1-5μm) were trapped in a focused ‘‘donut’’ laser beam, they rotated, due to the helical phase structure of the beam. Changing the polarization of the light from plane to circular caused the rotation frequency to increase or decrease, depending on the sense of the polarization with respect to the helicity of the beam. Rotation frequencies were obtained by Fourier analysis of amplitude fluctuations in the backscattered light from the particles. © 1996 The American Physical Society

Mechanical Effects of Optical Vortices

We concentrate on the forces and torques exerted on transparent and absorbing particles trapped in laser beams containing optical vortices. We review previous theoretical and experimental work and then present new calculations of the effect of vortex beams on absorbing particles

Combined application of bacteriophages with a competitive exclusion culture and carvacrol with organic acids can reduce Campylobacter in primary broiler production

For reducing Campylobacter (C.) in the food production chain and thus the risk to the consumer, the combined application of different measures as a multiple-hurdle approach is currently under discussion. This is the first study to investigate possible synergistic activities in vivo, aiming at reducing intestinal C. jejuni counts by administering (i) bacteriophages (phages) in combination with a competitive exclusion (CE) product and (ii) carvacrol combined with organic acids. The combined application of the two selected phages (Fletchervirus phage NCTC 12673 and Firehammervirus phage vB_CcM-LmqsCPL1/1) and the CE product significantly reduced C. jejuni loads by 1.0 log10 in cecal and colonic contents as well as in cloacal swabs at the end of the trial (33 and 34 days post hatch). The proportion of bacterial isolates showing reduced phage susceptibility ranged from 10.9% (isolates from cecal content) to 47.8% (isolates from cloacal swabs 32 days post hatch) for the Fletchervirus phage, while all tested isolates remained susceptible to the Firehammervirus phage. The use of carvacrol combined with an organic acid blend (sorbic acid, benzoic acid, propionic acid, and acetic acid) significantly reduced Campylobacter counts by 1.0 log10 in cloacal swabs on day 30 only