A minimum set of common principles for enabling Smart City Interoperability

The current investments for smart infrastructure development in cities result in the proliferation of self-consistent and closed applications (often called “silos”), which provide services with strong vertical integration but without ease of mutual horizontal integration. This paper investigates the state of several initiatives addressing this problem. It arrives at a proposal for diminishing and, ideally, breaking down these silos. This vision can be achieved by introducing the idea of building Smart Cities on a common set of architectural principles, Pivotal Points of Interoperability (PPI), and by applying these principles to the definition of a set of open Smart City Platform Specifications

Detection of the MyoD1 transcript in rhabdomyosarcoma cell lines and tumor samples by reverse transcription polymerase chain reaction

Rhabdomyosarcoma (RMS) is the most common soft-tissue sarcoma of childhood. Diagnosis of RMS can be difficult when it appears as a small round-cell tumor without evidence of differentiation. Recently, a set of regulatory proteins expressed during skeletal muscle development has been described. Among them, MyoD1 has been detected by Northern blot and immunohistochemical analyses in normal skeletal muscle and RMS. Given the relevance of this marker in the diagnosis of RMS, we developed an assay to evaluate the expression of MyoD1 mRNA in small tissue specimens by reverse transcription polymerase chain reaction. Specificity and sensitivity of the assay was determined in a series of 25 tumor cell lines and 39 pediatric tumor samples, including 35 RMSs. Subsequently, we studied the expression of MyoD1 in bone marrow and peripheral blood stem cell specimens. We detected the MyoD1 transcript in normal skeletal muscle and in almost all RMSs, whereas no expression was found in non-RMS samples or in normal hematopoietic tissues. This assay showed high sensitivity and specificity, and it could be a useful molecular tool for the diagnosis of RMS within small roundcell tumors of childhood and for the detection of minimal bone marrow and peripheral blood stem cell involvement in children with RMS, regardless of the histological subtype

Reversible Shaping of Microwells by Polarized Light Irradiation

In the last years, stimuli-responsive polymeric materials have attracted great interest, due to their low cost and ease of structuration over large areas combined with the possibility to actively manipulate their properties. In this work, we propose a polymeric pattern of soft-imprinted microwells containing azobenzene molecules. The shape of individual elements of the pattern can be controlled after fabrication by irradiation with properly polarized light. By taking advantage of the light responsivity of the azobenzene compound, we demonstrate the possibility to reversibly modulate a contraction-expansion of wells from an initial round shape to very narrow slits. We also show that the initial shape of the microconcavities can be restored by flipping the polarization by 90 degrees. The possibility to reversibly control the final shape of individual elements of structured surfaces offers the opportunity to engineer surface properties dynamically, thus opening new perspectives for several applications

Improved Long-Distance Polymerase Chain Reaction for the Detection of t(8;14)(q24;q32) in Burkitt’s Lymphomas

The t(8;14)(q24;q32), involving MYC gene (8q24) and the immunoglobulin heavy chain (IgH) locus (14q32), represents about 75% of all translocations in Burkitt’s lymphoma (BL). Due to the great variability of the breakpoint region, a standard polymerase chain reaction assay is not sufficient for the detection of this chromosomal translocation. The availability of new and more efficient DNA polymerases that allow the amplification of genomic fragments many kilobase-pairs long, makes it possible to identify the t(8;14) in BL cells by long-distance polymerase chain reaction (LD-PCR). We have established a simplified and efficient LD-PCR for the detection of t(8;14)(q24;q32) that relies on the use of one primer specific for MYC exon II combined, in different reactions, with four primers for the IgH locus: three for the constant regions Cμ, Cγ, and Cα, and one for the joining region (JH). We first studied seven BL cell lines and optimized LD-PCR reaction for analysis of tumor specimens. Five of seven cell lines were positive for the t(8;14), whereas two lines derived from endemic BL were negative, as expected. Of 15 biopsies obtained from pediatric BL and subsequently analyzed, 13 (87%) were positive for the translocation detected by LD-PCR and showed a product ranging in size from 2.0 to 9.5 kb. Cμ region was involved in 6 cases, Cγ and Cα in 2 cases each, and JH in 3 cases. Interestingly, 2 of the tumors positive for JH showed a second, larger PCR product with the Cα- and Cγ-specific primer, respectively. We established that our LD-PCR method could detect 10−3 BL cells within a population of hematopoietic cells lacking the translocation. In conclusion, our LD-PCR method represents a fast, highly sensitive, and specific tool to study sporadic BL and to detect minimal disease and residual disease in patients affected by t(8;14)-positive lymphomas