Correlation between the AMADEUS score and preoperative clinical patient-reported outcome measurements (PROMs) in patients undergoing matrix-induced autologous chondrocyte implantation (MACI)

Abstract Background Recently, the AMADEUS (Area Measurement And DEpth Underlying Structures) grading system has been introduced to evaluate and grade osteochondral lesions prior to cartilage surgery. The AMADEUS score has not been connected to clinical data in order to test a potential clincial impact. Purpose To examine the correlation between the AMADEUS score and preoperative patient-reported outcome measurements (PROMs). Study design Case series Methods Patients treated with matrix-induced autologous chondrocyte implantation (MACI) were included in the study, unless exclusion criteria like BMI > 35, prior extensive meniscectomy or ongoing inflammatory arthritis were present. Preoperative magnetic resonance (MR) examinations were graded according to the standardized AMADEUS protocol. The final AMADEUS score was correlated with preoperative patient-reported outcome measurements (PROMs), including the IKDC (International Knee Documentation Committee), the Lysholm score, the Short-Form-12 (SF-12) score, and the Core Outcome Measures Index (COMI) score. Results A total of 50 patients with a mean age of 33.6 ± 11.5 years, a mean BMI of 25.1 ± 4.9, and a mean defect size of 2.3 ± 1.5 cm2 were included in the study. More severe cartilage defects, indicated by the AMADEUS grade (R = 0.35, p = 0.01) and the AMADEUS score (R = − 0.36, p = 0.01) as well as larger chondral defects (R = 0.32, p = 0.03) show a moderate correlation with the higher COMI scores. No correlative capacity was demonstrated for the AMADEUS score and the IKDC, Lysholm, and Tegner activity scores as well as for its subscales. Conclusion There is a moderate correlation of the COMI and the AMADEUS score in patients treated with matrix-induced autologous chondrocyte implantation (MACI). All other patient-reported outcome measurement scores (PROMs) show no evidence of an association to the magnetic resonance-based AMADEUS score. Clinical relevance The clinical and scientific implication of the COMI score as a PROM tool can be recommended when working with the AMADEUS score and patients undergoing MACI

A paper-based platform with a pencil-drawn dual amperometric detector for the rapid quantification of ortho-diphenols in extravirgin olive oil

7siA simple, sensitive and fast responding device is described for the discrimination of hydrophilic ortho-diphenols, whose presence in abundant enough amounts is typical for extra virgin olive oils (EVOOs), from hydrophilic mono-phenols instead present in almost all vegetable oils. It consists of a dual electrode detector pencil-drawn at the end of a paper microfluidic channel, defined by hydrophobic barriers, where samples of these antioxidants, extracted from vegetable oils by a 80:20% v/v acetonitrile/water mixture, were applied. Thin-layer chromatographic runs conducted by using a 0.01 M H2SO4 + 1 M KCl running buffer allowed the selective detection of hydrophilic ortho-diphenols by profiting from the fact that they undergo reversible oxidation at less positive potentials than those required by monophenols for displaying their irreversible anodic process. On this basis, a potential for the oxidation of hydrophilic ortho-diphenols was applied to the upstream pencil-drawn electrode (W1) (at which a minor fraction of mono-phenols was also oxidized), while a potential for the reverse process involving the sole product (ortho-quinones) of the reversible oxidation of ortho-diphenols was imposed at the downstream pencil-drawn working electrode (W2). Thus, cathodic peak currents linearly dependent on analyte concentrations could be recorded at W2 which led to a satisfactory detection limit (8 μM, equivalent to 1.23 mg/L) even when working electrodes W1 and W2 with same dimensions were employed. Improved sensitivities and lower detection limits were achieved by increasing the dimensions of W2 with respect to W1, thanks to the improvement of the collection efficiency. Throughout this investigation, hydroxytyrosol (HTy) and tyrosol (Ty) were adopted as models of ortho-diphenols and mono-phenols, respectively, in view of their abundant presence in EVOOs. Real samples of EVOO from different production companies, of a simple olive oil and of a sunflower oil were analyzed. Different hydrophilic ortho-diphenol contents were found in EVOO samples (up to 40.8 mg/kg), while only a negligible amount turned out to be present in simple olive oil. No trace of these antioxidants were instead found in sunflower oil, as expected. All concentrations found were in good agreement with those detected by a more frequently employed spectrophotometric method used for the sake of comparison.reservedmixedDossi, N.; Toniolo, R.; Impellizzieri, F.; Tubaro, F.; Bontempelli, G.; Terzi, F.; Piccin, E.Dossi, Nicolo'; Toniolo, Rosanna; Impellizzieri, F.; Tubaro, Franco; Bontempelli, Gino; Terzi, F.; Piccin, E