Is the compact object associated with HESS J1731-347 a strange quark star?

The analysis of the central compact object within the supernova remnant HESS J1731-347 suggests that it has a small radius and, even more interestingly, a mass of the order or smaller than one solar mass. This raises the question of which astrophysical process could lead to such a small mass, since the analysis of various types of SN explosions indicate that is it not possible to produce a neutron star with a mass smaller than about $1.17 M_\odot$. Here we show that masses of the order or smaller than one solar mass can be obtained in the case of strange quark stars and that it is possible to build a coherent model explaining not only the mass and the radius of that object, but also its slow cooling suggested in various analyses. We also show that an astrophysical path exists which leads to the formation of such an object, and we discuss the role played in that scenario by strangelets assumed to constitute the dark matter.Comment: 6 pages, 2 Figures, 1 Table. New version significantly expanded. One section and one figure adde

Merger of a Neutron Star with a Black Hole: one-family vs. two-families scenario

A kilonova signal is generally expected after a Black Hole - Neutron Star merger. The strength of the signal is related to the equation of state of neutron star matter and it increases with the stiffness of the latter. The recent results obtained by NICER suggest a rather stiff equation of state and the expected kilonova signal is therefore strong, at least if the mass of the Black Hole does not exceed $\sim 10 M_\odot$. We compare the predictions obtained by considering equations of state of neutron star matter satisfying the most recent observations and assuming that only one family of compact stars exists with the results predicted in the two-families scenario. In the latter a soft hadronic equation of state produces very compact stellar objects while a rather stiff quark matter equation of state produces massive strange quark stars, satisfying NICER results. The expected kilonova signal in the two-families scenario is very weak: the Strange Quark Star - Black Hole merger does not produce a kilonova signal because, according to simulations, the amount of mass ejected is negligible and the Hadronic Star - Black Hole merger produces a much weaker signal than in the one-family scenario because the hadronic equation of state is very soft. This prediction will be easily tested with the new generation of detectors.Comment: 5 pages, 4 figure

Phenotypic characterization of human prostatic stromal cells in primary cultures derived from human tissue samples

Emerging evidence has shown that the tumor microenvironment plays a crucial role in prostate cancer (PCa) development and progression. However, the mechanism(s) through which stromal cells regulate epithelial cells and the differences among prostatic stromal cells of different histological/pathological origin in PCa progression remain unclear. Therefore, it is necessary to characterize the stromal cell populations present in benign prostatic hyperplasia (BPH) and PCa. To this end, we used cultures from stromal cells obtained from BPH-derived (15 cases) and PCa-derived (30 cases) primary cultures. In culture, stromal cells are a mixture of fibroblasts, myofibroblasts (MFs) and muscle cells. Fibroblasts are characterized for the expression of vimentin, MFs for the co-expression of α-smooth muscle actin (α-SMA) and vimentin, whereas muscle cells for the expression of α-SMA and desmin. Fibroblasts were present in large amounts in the BPH-compared to the PCa-derived cultures, whereas MFs were more representative of PCa-as opposed to BPH-derived cultures. Some α-SMA-positive cells retained the expression of basal cytokeratin K14. This population was defined as myoepithelial cells and was associated with senescent cultures. The percentage of MFs was higher in high-grade compared to moderate-and low-grade PCa-derived cultures, whereas the number of myoepithelial cells was lower in high-grade compared to moderate-and low-grade PCa-derived cultures. In addition, we analyzed the expression of p75NTR, as well as the expression of matrix metalloproteinase (MMP)-2, MMP-9 and tissue inhibitors of MMPs (TIMPs). p75NTR expression was elevated in the stromal cultures derived from PCa compared to those derived from BPH and in cultures derived from cases with Gleason scores.7 compared to those derived from cases with Gleason scores <7, as well as in cultures with a high concentration of MFs compared to those with a high concentration of fibroblasts. MMP-2 was secreted by all primary cultures, whereas MMP-9 secretion was observed only in some PCa-derived stromal cells, when the percentage of MFs was significantly higher compared to BPH-derived cultures. TIMP1, TIMP2 and TIMP3 were secreted in elevated amounts in the BPH-compared to the PCa-derived stromal cultures, suggesting the differential regulation of extracellular matrix (ECM) degradation. When we used 22rv1 and PC3 PCa xenograft models for the isolation and characterization of murine cancer-associated fibroblasts (CAFs) we noted that the angiogenic wave was concurrent with the appearance of a reactive stroma phenotype, as determined by staining for α-SMA, vimentin, tenascin, calponin, desmin and Masson's trichrome. In conclusion, MF stromal cells from PCa participate in the progression and metastasis of PCa, modualting inflammation, angiogenesis and epithelial cancer cell proliferation

Increased levels of DNA methyltransferases are associated with the tumorigenic capacity of prostate cancer cells

DNA methylation might be the earliest somatic genome changes in prostate cancer that also play an important role in the process of tumor invasion, growth and metastasis. In recent years, several inhibitors of DNA methyltransferases (DNMTis) have been developed and evaluated in pre-clinical models and in clinical trials. While these compounds are effective in the treatment of hematological conditions, clinical trials in solid tumors and in prostate cancer have shown limited or no efficacy. This may be attributed to inappropriate dose regimens leading to toxicity-related adverse events. As with other anti-target compounds, one of the obstacles encountered with DNMTis in prostate cancer could be the inability to select patients for the clinical studies as well as the inability to monitor the efficacy of the drug if not the conclusion of the study. Primary cultures derived from human prostatic tissues harvested from patients with benign prostatic hyperplasia (BPH) and prostate cancer (PCa) as well as neoplastic and non-neoplastic prostate cell lines were tested for DNMT expression/activity and to monitor azacitidine molecular efficacy. We observed that in primary cultures the levels of DNMT activity as well as the protein levels of DNMT1, DNMT3a and DNMT3b were higher in cultures derived from PCa compared to BPH tissue samples and significantly higher in cultures derived from PCa with Gleason scores ≥7 compared to those observed in cultures derived from Gleason scores <7. In addition, DNMT activity as well as DNMT1, DNMT3a and DNMT3b levels were higher in PCa cell lines compared to their non-neoplastic counterparts. Although DNMT activity was higher in high tumorigenic/aggressive PCa cell lines compared to low tumorigenic/aggressive cell lines, only the levels of DNMT3a and DNMT3b were significantly higher in the first group of cells, suggesting that DNMT1 activity is related to the transition to non-neoplastic versus neoplastic phenotype whereas the de novo methylation enzymes were mainly related to progression. Nevertheless, the comparison in the more aggressive PC3 cell derivatives (PC3-LN4 cells) also possessed higher levels of DNMT1 compared to PC3 and PC3M from which these cells were derived. Collectively, our results confirm previous data on the increased methylation in more aggressive tumors supporting the use of DNMTis in advanced prostate cancer. In addition, since glutathione S-transferase-π (GSTP1) was re-expressed or its protein levels were increased after treatment with non-toxic azacitidine doses and since GSTP1 can easily be measured in patient sera, the monitoring of this protein may aide in the evaluation of therapy in future clinical trials

Black Hole - Neutron Star mergers: using kilonovae to constrain the equation of state

The merging of a binary system involving two neutron stars (NSs), or a black hole (BH) and a NS, often results in the emission of an electromagnetic (EM) transient. One component of this EM transient is the epic explosion known as a kilonova (KN). The characteristics of the KN emission can be used to probe the equation of state (EoS) of NS matter responsible for its formation. We predict KN light curves from computationally simulated BH-NS mergers, by using the 3D radiative transfer code \texttt{POSSIS}. We investigate two EoSs spanning most of the allowed range of the mass-radius diagram. We also consider a soft EoS compatible with the observational data within the so-called 2-families scenario in which hadronic stars coexist with strange stars. Computed results show that the 2-families scenario, characterized by a soft EoS, should not produce a KN unless the mass of the binary components are small ($M_{\rm BH} \leq 6M_{\odot}$, $M_{\rm NS} \leq 1.4M_{\odot}$) and the BH is rapidly spinning ($\chi_{\rm BH} \geq 0.3$). In contrast, a strong KN signal potentially observable from future surveys (e.g. VRO/LSST) is produced in the 1-family scenario for a wider region of the parameter space, and even for non-rotating BHs ($\chi_{\rm BH} = 0$) when $M_{\rm BH} = 4M_{\odot}$ and $M_{\rm NS} = 1.2M_{\odot}$. We also provide a fit that allows for the calculation of the unbound mass from the observed KN magnitude, without running timely and costly radiative transfer simulations. Findings presented in this paper will be used to interpret light curves anticipated during the fourth observing run (O4), of the advanced LIGO, advanced Virgo and KAGRA interferometers and thus to constrain the EoS of NS matter.Comment: 14 pages, 16 figures, 2 table

Efficiency measures of emergency departments: an Italian systematic literature review

Life expectancy globally increased in the last decades: the number of people aged 65 or older is consequently projected to grow, and healthcare demand will increase as well. In the recent years, the number of patients visiting the hospital emergency departments (EDs) rocked in almost all countries of the world. These departments are crucial in all healthcare systems and play a critical role in providing an efficient assistance to all patients. A systematic literature review covering PubMed, Scopus and the Cochrane Library was performed from 2009 to 2019. Of the 718 references found in the literature research, more than 25 studies were included in the current review. Different predictors were associated with the quality of EDs care, which may help to define and implement preventive strategies in the near future. There is no harmonisation in efficiency measurements reflecting the performance in the ED setting. The identification of consistent measures of efficiency is crucial to build an evidence base for future initiatives. The aim of this study is to review the literature on the problems encountered in the efficiency of EDs around the world in order to identify an organisational model or guidelines that can be implemented in EDs to fill inefficiencies and ensure access optimal treatment both in terms of resources and timing. This review will support policy makers to improve the quality of health facilities, and, consequently of the entire healthcare systems