Genetics, Cytogenetics, and Epigenetics of Colorectal Cancer

Most of the colorectal cancer (CRC) cases are sporadic, only 25% of the patients have a family history of the disease, and major genes causing syndromes predisposing to CRC only account for 5-6% of the total cases. The following subtypes can be recognized: MIN (microsatellite instability), CIN (chromosomal instability), and CIMP (CpG island methylator phenotype). CIN occurs in 80–85% of CRC. Chromosomal instability proceeds through two major mechanisms, missegregation that results in aneuploidy through the gain or loss of whole chromosomes, and unbalanced structural rearrangements that lead to the loss and/or gain of chromosomal regions. The loss of heterozygosity that occur in the first phases of the CRC cancerogenesis (in particular for the genes on 18q) as well as the alteration of methylation pattern of multiple key genes can drive the development of colorectal cancer by facilitating the acquisition of multiple tumor-associated mutations and the instability phenotype

The Establishment of an Organic Farmers’ Market as a Training Case Study and Research for Graduate Students of Organic Agriculture at the University of Palermo

 Despite the modest regional consumption of organic products, Sicily is one of the most important European regions in surface area per number of companies active in organic farming. The University of Palermo, as early as 2000, to promote the development and success in this industry, the Faculty of Agriculture, introduced two major degree programs in "Organic Farming". The objective of these courses is to train graduates who are technically capable of supporting the organic farmers market, to promote and facilitate the expansion of the consumption of organic products, and to create employment opportunities for young graduates. At the end of 2009, in the area of these activities, a training and research program involved numerous students in the creation of an "joint consumption" association whose purpose is the expansion of the consumption of organic products through the enhancement of a direct relationship with small organic farms who would be unlikely to penetrate large markets, or have relationships with large distribution networks.The project aims to assess the ability of the students of the Faculty to transmit the knowledge acquired in the program, and to investigate the relative issues regarding the new phenomenon emerging in recent years in Italy, represented by the spread of various forms of ethically-conscious consumption groups called "Gruppi di Acquisto Solidale (GAS)" or Solidarity Purchase Groups

Fertility in an urban context. A complex phenomenon

Quaderni di ricerca Ires ; n.102- Contents #4- Having Children in a Big City: Different Social Groups, Different Conditions, Different Behaviours #6- Introduction #11- The Turin Longitudinal Study for the Analysis of Fertility #13- Turin: the Urban Context #25- The Changing Relationship between Female Labour Force Participation and Fertility #47- Lowest-Low Fertility in an Urban Context. When Migration Plays a Key Role #62- Summary and Conclusion #8

Efficacy and Feasibility of the Epithelial Cell Adhesion Molecule (EpCAM) Immunomagnetic Cell Sorter for Studies of DNA Methylation in Colorectal Cancer

The aim of this work was to assess the impact on measurements of methylation of a panel of four cancer gene promoters of purifying tumor cells from colorectal tissue samples using the epithelial cell adhesion molecule (EpCAM)-immunomagnetic cell enrichment approach. We observed that, on average, methylation levels were higher in enriched cell fractions than in the whole tissue, but the difference was significant only for one out of four studied genes. In addition, there were strong correlations between methylation values for individual samples of whole tissue and the corresponding enriched cell fractions. Therefore, assays on whole tissue are likely to provide reliable estimates of tumor-specific methylation of cancer genes. However, tumor cell tissue separation using immunomagnetic beads could, in some cases, give a more accurate value of gene promoter methylation than the analysis of the whole cancer tissue, although relatively expensive and time-consuming. The efficacy and feasibility of the immunomagnetic cell sorting for methylation studies are discussed

TOWARDS THE DEVELOPMENT OF BENCH TESTING FOR LOWER-LIMB PROSTHETIC SOCKETS FOR SPORT APPLICATIONS

Prosthetic sockets are the bespoken part of lower-limb prostheses. Knowledge about the mechanical properties of sockets is essential to ensure patient safety and comply with current medical device regulations. This includes sockets designed for sport activities. Unfortunately, the literature is extremely limited and contradictory as described in a recent systematic review. The aim of this study was to initiate a research activity aiming to design a mechanical bench system for socket testing and perform a comparative analysis of the ultimate strength of alternative socket layups. Results highlight substantial differences in the maximum loading at failure, stressing the importance of increasing the knowledge about socket mechanical properties to support prosthetists provide reliable and safe products to patients and athletes