26 research outputs found

    Student and supervisor perspectives in a computer-mediated research relationship

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    A brief narrative description of the journal article, document, or resource. This paper presents the reflective perspectives of the student and supervisor in a successful computer-mediated research relationship at Deakin University (Australia). Key contributing factors are discussed in a dialog format covering the role of computer-mediated communication (CMC), the projection of social presence, student self-efficacy beliefs, the role of information and communication technology (ICT), and interaction in online professional networks. Drawing on relevant theory, inherent challenges are addressed, informing some concluding suggestions as to how supervision might become more responsive to the emergent forms of research learning being experienced by escalating numbers of postgraduate students studying at a distance via ICT.<br /

    Transfection of primary human skin fibroblasts by electroporation

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    Primary human skin fibroblasts are an accessible source of phenotypically and karyotypically normal human cells, but are difficult to transfect with exogenous DNA. Here we demonstrate that both transient expression and stable transformation can be carried out by the method of electroporation. Highly efficient transient chloramphenicol acetyltransferase expression was shown after transfection with plasmid pRSVCAT. Stable transformation of human skin fibroblasts to G418 resistance was obtained after electroporation with neo-containing plasmids at an efficiency of approximately 1.4 x 10-5/[mu]g DNA. The ability to easily transfect these cells with exogenous DNA may have important applications in the study of human genetic diseases and cancer.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/27179/1/0000177.pd

    MICROCOSM: An Open Model for Hypermedia With Dynamic Linking

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    There are currently a number of commercially available hypertext and hypermedia systems, of varying levels of sophistication and usability, but there are still many problems to be resolved in the design of such systems. In this paper, we itemise some of the major problems that we have identified as possibly causing a barrier to the growth and development of hypermedia applications outside the research community. A model of an open hypermedia architecture with dynamic linking features is proposed that moves some way to resolving these problems, and the first implementation of the system, Microcosm, is presented and discussed
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