Verwendung von Gen-Amplifikation zur Bestimmung der Reservoire von M.leprae in der Umwelt und als Therapiekontrolle: Eine Alternative zu Tierversuchen Schlussbericht

The causative agent of leprosy cannot be cultivated up to now. Animal experiments are necessary for the estimation of M. leprae. The polymerase chain reaction (PCR) was introduced for the diagnosis of M. leprae, but the results are variable. To replace the animal experiments, the standardization of this method is needed. In this project, the PCR has been precised and two pairs of primers (242 and 372 bp) established, which enhanced the specificity and sensitivity. The hybridization as a control method is not necessary. The PCR can replace the animal experiments and furthermore, requires only 2 days for estimation of M. leprae. (orig.)SIGLEAvailable from TIB Hannover: F94B1472 / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekBundesministerium fuer Forschung und Technologie (BMFT), Bonn (Germany)DEGerman

Ermittlung multivariater Struktur-Toxizitaetsbeziehungen umweltrelevanter Biotestsysteme und Chemikalienklassen, die dem Chemikaliengesetz unterliegen. T. A und B Bakterientest (E.coli). Durchfuehrung von Multiregressions- und Multivariaten Datenanalysen fuer 12 Testsysteme

TIB Hannover: RN 8908(89-050) / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekSIGLEDEGerman

Immunchemischer und immunbiologischer Nachweis von endogenem Pyrogen/Interleukin 1 Abschlussbericht

SIGLETIB: FR 4668(2) / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekDEGerman

Entwicklung neuer Chemotherapeutika fuer klinisch relevante Problemkeime auf der Basis gesicherter Mechanismen und unter Einbeziehung moderner Methoden der rationalen Wirkstoffentwicklung Schlussbericht

In vitro test systems for testing inhibitors of the folate biosynthesis of C.albicans have been developed successfully. Supported by quantitative structure-activity relationship analysis sulfones and sulfonamides have been optimized as inhibitors of pteroate synthase of C.albicans. New benzylpyrimidines have been designed an synthesized as inhibitors of C.albicans dihydrofolate reductase, wich are 380 times more active than trimethoprim (patent pending). The inhibitory activity against whole cell culture is, however, disappointingly low as the compounds are unable to pass sufficiently the membrane of C.albicans. To overcome the multidrug resistance of mycobacteria new potential inhibitors of ribonucleosidediphosphate reductase have been designed and synthesized as partners in combination with rifampicin. The new heterocyclic hydrazones are inhibitors of mycobacteria and show strong synergism when combined with rifampicin. The new compounds cannot yet be used clinically. Therefore rifampicin was combined with thiacetazone which shows also strong synergism. The clinical results are very promising. Patents were received for the new combinations. (orig.)Available from TIB Hannover: F95B687+a / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekSIGLEBundesministerium fuer Forschung und Technologie (BMFT), Bonn (Germany)DEGerman