7-Chloro-2-[1-(4-methoxy­phen­yl)pyrazol-4-yl]-3,3-dimethyl-3H-indole

In the title compound, C20H18ClN3O, the dihedral angle between the pyrazole and the 3H-indole components is only 13.28 (6)°, indicating that there is conjugation between the two heterocyclic subunits. The N-methoxy­phenyl unit makes a dihedral angle of 25.10 (7)° with the pyrazole ring

A novel hydrogel based microextraction of analytes

AbstractWe report on a new method for the preconcentration and microextraction of analytes from aqueous samples using a pH-sensitive hydrogel. It is referred to as semisolid–liquid dispersive microextraction (SSLDM) and has the advantages of both dispersive liquid–liquid microextraction (DLLME) and solid-phase extraction (SPE). The surface area of the droplets is large, so the equilibrium state is quickly reached. The high density of the gel facilitates phase separation, and disadvantages of existing methods are overcome in that there is no need for extraction and dispersive solvents (as in conventional DLLME) and no need for desorption (as in SPE). The SSLDM method integrates sampling, extraction and concentration into one single and solvent-free step. The method was exemplarily applied to the preconcentration of malachite green (MG) and crystal violet (CV) which then were quantified by spectrophotometry. Specifically, poly (styrene-alt-maleic acid), a pH-sensitive hydrogel, was used to extract MG and CV from water samples. The linear analytical range is from 0.01 to 1μmolL−1 for MG, and from 0.05 to 1μmolL−1 for CV. The correlation coefficient for MG and CV is 0.999 and 0.993, respectively. The limit of detection for MG and CV is 0.011 and 0.014μmolL−1, respectively

Gold nanoparticles biofunctionalized (grafted) with chiral amino acids: a practical approach to determining the enantiomeric percentage of racemic mixtures

The importance of stereochemistry in chiral drugs is due to the different pharmacological behavior of enantiomers.</p

Evaluating the Safety and Efficacy of MSCs-derived Exosomes for Treatment of Resistant Perianal Fistula in IBD patients; Clinical Trial Phase I

Background and objectives: Exosomes therapy is a promising technique that can decrease the concern related to the fate of transplanted stem cells to the fistula location. The unique properties of mesenchymal stem cells (MSCs)-derived exosomes can improve fistula healing because of their high ability to regulate and modify immune responses. This project aims to examine the safety and efficacy of implementing MSCs-exosomes in treating refractory fistulas in inflammatory bowel disease (IBD) patients. Methods: The exosomes were isolated from umbilical cord-derived MSCs and characterized using flow cytometry, western blotting, and transmittance electron microscope (TEM). Five patients (three men and two women) with refractory perianal Crohn’s disease with an average age of 35.4 years old were included. The patients did not match the exclusion criteria, and the presence of refractory fistulas was the main inclusion criteria. These patients have their exosomes injections in the operation room. The therapy responses of patients were assessed using physical examination, face-to-face interviews, computerized tomography (CT) scan, and magnetic resonance imaging (MRI) six months later. Result: The Following injection, patients reported a 70% improvement on average. Two patients claimed complete healing after exosome injections, while one patient reported no improvement and active discharge from the fistula site. In addition, five patients reported no adverse effects, both systemic or on the injection site. Conclusion: Injection of exosomes extracted from MSCs shows total safety and a satisfactory therapeutic impact, as shown in this and other research, and could play a significant role in the treatment of gastrointestinal fistulas in the future.</jats:p

Evaluating the Safety and Efficacy of MSCs-derived Exosomes for Treatment of Refractory Perianal Fistula in IBD patients; Clinical Trial Phase I

Background and objectives: Exosomes therapy is a promising technique that can decrease the concern related to the fate of transplanted stem cells to the fistula location. The unique properties of mesenchymal stem cells (MSCs)-derived exosomes can improve fistula healing because of their high ability to regulate and modify immune responses. This project aims to examine the safety and efficacy of implementing MSCs-exosomes in treating refractory fistulas in inflammatory bowel disease (IBD) patients. Methods: The exosomes were isolated from umbilical cord-derived MSCs and characterized using flow cytometry, western blotting, and transmittance electron microscope (TEM). Five patients (three men and two women) with refractory perianal Crohn’s disease with an average age of 35.4 years old were included. The patients did not match the exclusion criteria, and the presence of refractory fistulas was the main inclusion criteria. These patients have their exosomes injections in the operation room. The therapy responses of patients were assessed using physical examination, face-to-face interviews, computerized tomography (CT) scan, and magnetic resonance imaging (MRI) six months later. Result: The Following injection, patients reported a 70% improvement on average. Two patients claimed complete healing after exosome injections, while one patient reported no improvement and active discharge from the fistula site. In addition, five patients reported no adverse effects, both systemic or on the injection site. Conclusion: Injection of exosomes extracted from MSCs shows total safety and a satisfactory therapeutic impact, as shown in this and other research, and could play a significant role in the treatment of gastrointestinal fistulas in the future.</jats:p