Postharvest Ozone Fumigation of Grapes (cv Sangiovese) Differently Affects Volatile Organic Compounds and Polyphenol Profiles of Berries and Wine

Consumers are more and more oriented towards the purchase of safer food and beverages, which is pushing the wine sector to find alternatives to the use of sulfur dioxide. Ozone (O3) is already applied in the wine industry to produce sulfur dioxide-free wines through the patented method Purovino®. The aim of this two-year study was that of evaluating whether the postharvest treatment of grapes with ozone affects volatile organic compounds (VOCs) and polyphenol profile in berries, and in turn, wine composition. Grape bunches (Vitis vinifera L.) of cv Sangiovese were fumigated overnight with gaseous ozone (max 20 g·h−1 with 6% w.w−1 of ozone) in a cold room at 4°C (±0.5). After treatment, grapes were processed into wine. In grapes, ozone treatments increased total polyphenol and flavonoid content and upregulated specific genes (phenylalanine ammonia lyase, VvPAL, flavanol synthase 1, and VvFLS1) involved in polyphenol biosynthesis. Wine obtained from ozone-treated grapes had higher flavanol content than the control. Fumigation only slightly affected the different VOC classes of grapes and wine, including aroma compounds derived from the lipoxygenase (LOX) pathway. Although a season-dependent effect was observed, results showed that postharvest ozone treatments applied to avoid the use of sulfur dioxide introduced limited but, in general, positive modifications to grape and wine composition. This information provides assurance to winemakers that the maintenance of wine quality and typicity will be guaranteed when using ozone treatments

Innovative application of NIR-AOTF and MRI to study water behaviour in cut flower

In order to study the water status of cut flowers, a comparison study was made between flowers stored in water and flowers stored «dry pack», by using a portable NIR (near infrared)-AOTF(acousto-optical tunable filter) instrument and MRI (magnetic resonance imaging). As model flower, Zantedeschia aethiopica (commercially known as Calla lily) was used. To predict the weight loss and water content by NIR-AOTF, cut flowers were dried in a cold room at 10°C (±1°C) and 85% (±5%) relative humidity (RH), and measured for weight loss. For MRI application, 4 and 20°C storage temperatures were used for flowers kept in water or dry; two stem sections, basal and middle, were measured. Significant correlation results for weight loss and water content ( R2 in calibration = 0.98 for estimated % of water loss and 0.96 for % of water content; R2 cv = 0.95 and 0.90) were obtained by NIR-AOTF spectra acquisitions. MRI detected vessel degradation in the stem of the water-stored flowers at 4°C but at 20°C in dry storage no vessel degradation appeared and images were correlated with dry matter values. The use of image software allowed the transformation of images in normalized population and pixel intensity, which gave hints about the potential use of these data to combine with NIR-AOTF data. NIR-AOTF, an easy-to-use and non destructive instrument, can be used to predict the vase life of cut flowers by measuring water content or weight loss. MRI is a powerful tool to identify the plugging of vessels but it is destructive; the image software used represents a useful tool to correlate MRI with NIR-AOTF to predict vessel plugging

Interplay between Nox2 activity and platelet activation in patients with sepsis and septic shock. a prospective study

Background. Although preclinical studies highlighted the potential role of NADPH oxidase (NOX) in sepsis, only few studies evaluated the oxidative stress in patients with sepsis and septic shock. The objective of the study is to appraise the oxidative stress status and platelet function in patients with sepsis and septic shock compared to healthy controls. Methods and Results. Patients with sepsis or septic shock admitted to the hospital Policlinico Umberto I (Sapienza University, Rome) underwent a blood sample collection within 1 hour from admission. Platelet aggregation, serum thromboxane B2 (TxB2), soluble NOX2-derived peptides (sNox2-dp), and hydrogen peroxide breakdown activity (HBA) were measured and compared to those of healthy volunteers. Overall, 33 patients were enrolled; of these, 20 (60.6%) had sepsis and 13 (39.4%) septic shock. Compared to healthy controls (n=10, age 67.8±3.2, male 50%), patients with sepsis and septic shock had higher platelet aggregation (49% (IQR 45-55), 60% (55.75-67.25), and 73% (IQR 69-80), respectively, p<0.001), higher serum TxB2 (77.5 (56.5-86.25), 122.5 (114-131.5), and 210 (195-230) pmol/L, respectively, p<0.001), higher sNox2-dp (10 (7.75-12), 19.5 (17.25-21), and 33 (29.5-39) pg/mL, respectively, p<0.001), and lower HBA (75% (67.25-81.5), 50% (45-54.75), and 27% (21.5-32.5), respectively, p<0.001). Although not statistically significant, a trend in higher levels of serum TxB2 and sNox2-dp in patients who died was observed. Conclusions. Patients with septic shock exhibit higher Nox2 activity and platelet activation than patients with sepsis. These insights joined to better knowledge of these mechanisms could guide the identification of future prognostic biomarkers and new therapeutic strategies in the scenario of septic shock