Linkage Mapping of Stem Saccharification Digestibility in Rice

Rice is the staple food of almost half of the world population, and in excess 90% of it is grown and consumed in Asia, but the disposal of rice straw poses a problem for farmers, who often burn it in the fields, causing health and environmental problems. However, with increased focus on the development of sustainable biofuel production, rice straw has been recognized as a potential feedstock for non-food derived biofuel production. Currently, the commercial realization of rice as a biofuel feedstock is constrained by the high cost of industrial saccharification processes needed to release sugar for fermentation. This study is focused on the alteration of lignin content, and cell wall chemotypes and structures, and their effects on the saccharification potential of rice lignocellulosic biomass. A recombinant inbred lines (RILs) population derived from a cross between the lowland rice variety IR1552 and the upland rice variety Azucena with 271 molecular markers for quantitative trait SNP (QTS) analyses was used. After association analysis of 271 markers for saccharification potential, 1 locus and 4 pairs of epistatic loci were found to contribute to the enzymatic digestibility phenotype, and an inverse relationship between reducing sugar and lignin content in these recombinant inbred lines was identified. As a result of QTS analyses, several cell-wall associated candidate genes are proposed that may be useful for marker-assisted breeding and may aid breeders to produce potential high saccharification rice varieties

Effects of spermidine synthase overexpression on polyamine biosynthetic pathway in tobacco plants

Transgenic tobacco plants overexpressing the Datura stramonium spermidine synthase (EC 2.5.1.16) cDNA were produced in order to understand the role of this gene in the polyamine metabolism and in particular in affecting spermidine endogenous levels. All the analysed transgenic clones displayed a high level of overexpression of the exogenous cDNA with respect to the endogenous spermidine synthase. No relationship was detected between the mRNA expression level of S-adenosylmethionine decarboxylase (SAMDC, EC 4.1.1.50), which did not change between the negative segregant control and the transgenic plants, and spermidine synthase, suggesting the existence of an independent regulatory mechanism for transcription of the two genes. The determination of enzyme activities indicated an increased spermidine synthase and S-adenosylmethionine decarboxylase activity, with the last being mainly recovered in the particulate fraction. ODC (ODC, EC 4.1.1.17) was the most active enzyme and its activity was equally distributed between the soluble and the particulate fraction, while ADC (ADC, EC 4.1.1.19) activity in the transgenic plants did not particularly change with respect to the controls. In comparison to the controls, the transformed plants displayed an increased spermidine to putrescine ratio in the majority of the clones assayed, while the total polyamine content remained almost unchanged. These findings suggest a high capacity of the transformed plants to tightly regulate polyamine endogenous levels and provide evidence that spermidine synthase is not a limiting step in the biosynthesis of polyamines

Jasmonate and Na-orthovanadate promote resveratrol production in Vitis vinifera cv. Barbera cell cultures

Here the effect of jasmonic acid, methyljasmonate and Na-orthovanadate on the production of resveratrol was studied in Vitis vinifera cv. Barbera cell suspension cultures. \u2022 Na-orthovanadate at 0.1 mM and 1 mM concentration was efficient in promoting the production and/or accumulation and release in the culture medium of cis-resveratrol while trans-resveratrol levels were not affected by this treatment. \u2022 Methyljasmonate was highly effective in stimulating both trans- and cis-resveratrol endogenous accumulation, as well as their release into the culture medium. Cis-resveratrol was absent or detected in very low amounts in the controls. Jasmonic acid was less efficient than methyljasmonate in promoting endogenous resveratrol accumulation, but it stimulated the release in the culture medium especially of cis-resveratrol. \u2022 Gel analysis was performed on control and 10 \u3bcM MeJA treated cell suspensions. Results showed an up-regulation of the stilbene synthase demonstrating that MeJA stimulated the synthesis ex-novo of this protein

Influence of the Z = 50 shell closure on heavy-ion sub-barrier fusion

The systematics of the lowest 2$$^+$$ energies of even-even nuclei, and the B(E2) rates connecting those levels to the ground states, give essential information about the degree of collectivity in various regions of the nuclide chart. The B(E2) rates provide crucial tests of theoretical predictions, such as the quenching of shell structure in neutron-rich nuclei. Measurements of such rates are in progress using multi-nucleon transfer reactions and radioactive ion beams. We have measured the fusion excitation functions for the three systems $$^{48}$$Ca + $$^{116}$$Cd,$$^{118}$$Sn,$$^{120}$$Te, in the relevant energy range from above to below the Coulomb barrier. The magic projectile $$^{48}$$Ca has been chosen in view of its spherical and rigid structure, while the three targets have proton numbers $$Z= 48,50,52$$. We observe that the cross sections are smallest for the target $$^{118}$$Sn, thus indicating the effect on sub-barrier fusion of crossing the major shell closure at Z = 50. This suggests the possibility of using near-barrier fusion reactions of radioactive ion beams with suitable stable targets, as a complementary technique to evidence the low-lying nuclear structure of exotic nuclei, besides well established methods

The maize ZmMYB42 represses the phenylpropanoid pathway and affects the cell wall structure, composition and degradability in <i>Arabidopsis thaliana</i>

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