2 research outputs found
Trichinella spiralis : Stimulation of mast cells by TSL-1 antigens trigger cytokine mRNA expression and release of IL-4 and TNF through an Ig-independent pathway
International audiencePrevious in vitro studies have demonstrated that mast cells (MC) can be directly activated by Trichinella spiralis larvae 1 (TSL-1) antigens. To characterize even more this activation of MC and their possible role on induction and regulation of the Type 2 response generated against T spiralis infection, we studied the interaction between a hybrid rat MC line (HRMC), murine bone marrow MC (mBMMC), and TSL-1 antigens. Immunofluorescence staining and flow cytometry analysis showed that TSL-1 antigens bound to the surface of HRMC cells, resulting in the transcriptional induction and in the release of TNF and IL-4. Besides, an increase of IL-4 intracellular expression was also observed in mBMMC. This Suggests that MC may play an important role in the early immune response against T spiralis and may be a source of cytokines, that regulate the final onset of the immune mechanisms which determine the course of the infection
Identification, molecular characterisation and differential expression of caveolin-1 in Trichinella spiralis maturing oocytes and embryos
International audienceCaveolins are integral membrane proteins implicated in cholesterol homeostasis and transport, endocytosis mechanisms and regulation of signal transduction in differentiated cells. In this work a caveolin-1 gene from the nematode Trichinella spiralis (Ts-cav-1) was cloned and identified as an adult-specific antigen. For this, a cDNA library of T spiralis 3-day-old adult worms was screened using a stage-specific cl)NA-labelled probe. One positive clone contained a cDNA insert of 1427-bp and a full-length open reading frame (ORF) of 687-bp, which encodes for a 229 amino acid polypeptide with a theoretical molecular weight of 26 kDa. BLAST and FASTA searches revealed a 36W% and 57% identity with Caenorhabditis elegans caveolin-1, respectively. Confocal laser microscopy analysis using antibodies generated against Ts-CAV-1 protein and cross-sections of adult parasites showed that Ts-CAV-1 gradually accumulates on the surface of Trichinella oocytes and embryos, reaching a maximum at 3 days p.i., and decreasing during new-born larvae (NBL) development. RT-PCR assays of parasites from 1 to 4 days p.i. showed a similar gene expression profile to that observed for Ts-CAV-1 which suggests a specific developmental regulation. Free cholesterol was mainly distributed in the female germ line and it displayed increasing membrane accumulation, similar to the pattern obtained for Ts-CAV-1 protein, which suggests a temporal membrane association with Ts-CAV-1 that in turn will perform the functions mentioned above. Our results strongly indicate that Ts-cav-1 from T. spiralis plays a role in oocyte maturation and embryogenesis during development, demonstrating gender-specific expression