8 research outputs found
Solvent Effects on the Dynamics of Amyloidogenic Insulin Revealed by Neutron Spin Echo Spectroscopy
La flora del litorale pescarese come indicatore biologico dello stato ambientale e delle sue trasformazioni
Thioflavin T Promotes AÎČ(1â40) Amyloid Fibrils Formation
Fibrillogenesis of the small peptide AÎČ(1â40) is considered to be the
hallmark of Alzheimerâs disease. Some evidence indicates small oligomers, rather than mature fibrils, as the key cytotoxic agents. The fluorescent dye Thioflavin T (ThT) is often used to detect amyloid deposits in both in vivo and in vitro experiments, and it is used to study kinetic measurements, under the fundamental hypothesis that this probe does not influence the aggregation processes. We report experimental data showing that ThT may
promote the AÎČ(1â40) peptide amyloid aggregation changing solventâpeptide interactions and stabilizing more ordered ÎČ-like conformation. This finding has a two-fold importance: It is a fundamental warning in all fibrillation experiments where ThT is used as fluorescent probe, and it suggests that ThT, accelerating fibril formation, could be used to reduce the presence of transient small oligomers, thus interfering with the pathogenic impact of AÎČ
peptide