Mastitis Acuta in a Pure Breed Cane Corso female. A Case Report

Data regarding bitch mastitis is fairly scarce compared to the literature regarding ruminant mastitis. Neglecting clinical and subclinical cases of mastitis can be life threatening for both dam and puppies. The aim of this report is to present a case of Mastitis acuta complicated with a case of neonatal septicaemia in a Cane Corso (Canis lupus familiaris L.) pure breed female. Laboratory assays showed a milk pH value of 7.5, milk cytology revealed the presence of segmented neutrophils, while foamy cells, phagocytosis and highly pathogenic bacteria (i.e. Escherichia coli and Pseudomonas aeruginosa) were isolated from milk and puppy. To the author's knowledge, this is the first case of bitch mastitis linked to neonatal septicaemia with mixed E. coli and P. aeruginosa infection

Interdependence of productive effort and in vitro vegetal extract treatment on specific cell-mediated immunity in horses

The research aimed at identifying and rendering alcoholic extracts of various plants based on their in vitro potential in stimulating cell mediated immunity and in alleviating stress effects subsequent to workout type in horses. Experimental horses were selected from different working environments: (a) draft, agricultural works-n = 16, average age 8 years, (b) inconstant effort, leisure, n = 15, average age 7.5 years and (c) constant training, endurance, average age 3.5 years. Blood samples were collected in heparinized vials (50 UI/ml) before and after the workout. Alcoholic extracts (2 mu l/well, duplicate) of Taraxacum officinale (L) Weber, Symphytum officinale L., Equisetum palustre L., Viola tricolor L., Avena sativa L., Capsella bursa pastoris (L) Medik., Hypericum perforatum L., Chelidonium majus L. were investigated by blast transformation test and statistically interpreted (Students t test). In vitro responses were the highest to all tested extracts in endurance horses and the lowest in draft horses (p<0.01-0.001). C. bursa pastoris. was the most efficient in draft and endurance categories before the workout (draft 45.13 +/- 8.05, in leisure 48.09 +/- 24.13 and 53.44 +/- 9.32 in endurance horses), but not after the workout, where C. majus performed better (41.86 +/- 22.47 in leisure and 48.1 +/- 6.82 in endurance horses), probably due to its complex, protein-rich latex structure. The effects of plant extracts depended on the type of effort and the level of constant training rather than on the taxonomy of the plant

Unravelling the Antioxidant Potential of Resveratrol and Quercetin in Animal Models: A Comprehensive Review

Resveratrol and quercetin are naturally occurring polyphenolic compounds widely studied for their potential health benefits, particularly their antioxidant properties. This abstract provides an overview of the extensive research conducted on resveratrol and quercetin as antioxidants in animal models, highlighting their mechanisms of action and therapeutic potential. Animal models, such as rodents, have been instrumental in elucidating the oxidative stress pathway and evaluating the efficacy of various antioxidants. Resveratrol and quercetin have demonstrated significant antioxidant effects in animal models through multiple mechanisms. These include direct scavenging of reactive oxygen species (ROS), upregulation of endogenous antioxidant enzymes, inhibition of lipid peroxidation, and modulation of oxidative stress-related signaling pathways

Enhanced responses in antigen primed dogs after administration of a complex microbial immune stimulating compound

The research aimed to investigate the efficacy of antiparvoviral monovalent vaccine for puppies treated with a complex poly-bacterial compound. The experiment was carried out on 17 Labrador Retriever puppies, from 41 to 63 days of age. The two groups (experimental and control) were subjected to: a) a polybacterial preparation and antiparvoviral vaccine (n = 12) and b) to antiparvoviral vaccine (n = 5), respectively. One ml of a poly-bacterial preparation was administered on days 0, 2, 4, 6, while the antiparvoviral vaccine Duramune KF11 was injected on day 8 of the experiment. Phagocytic activity and total lysozyme concentrations were measured on days 0, 8 and 22 of the experiment. The global immunostimulating effects of the poly-bacterial preparation on the innate immune system of vaccinated puppies increased when compared to those of the control variant

Potential use of the stinging nettle as an enhancer of weight gain and innate immune response in broiler chickens

Researches were conducted to investigate the in vivo effect of an nettle plant alcohol extract on the phagocytosis and body weight in 48 days old broiler chickens (n=34). The birds were divided into three groups: subcutaneously injected with 0.5 ml of saline (control, n = 12), with 0.5 ml of alcohol (group II, n=11) or with 0.5 ml of alcoholic nettle extract (group III, n=11). Sterile heparinized blood, sampled on days 0, 7 and 13, was processed by an in vitro carbon particle inclusion test, the phagocytosis being expressed in optical density units. The birds were weighed simultaneously with the blood sampling. We concluded that a single nettle extract treatment does not significantly influence the phagocytosis, while the second administration intensified it. The alcoholic nettle extract had a beneficial effect on the body weight of broiler chickens

Comparative evaluation of florfenicol and polymeric nanopar-ticles loaded with florfenicol against bacterial strains isolated from chickens: insights into antimicrobial resistance

Antimicrobial resistance (AMR) poses a significant threat to both human and animal health, necessitating the search for alternative antimicrobial agents and strategies. In this study, we aimed to identify and isolate clinical bacterial strains from chickens and evaluate their sensitivity to florfenicol, a common antimicrobial agent that is used exclusively in veterinary medicine, along with polymeric nanoparticles loaded with florfenicol at various concentrations. Three clinical bacterial strains were successfully isolated and identified from chicken presenting clinical signs. In order to assess their susceptibility, the isolated strains were subjected to a standard disc diffusion assay using florfenicol. Subsequently, polymeric nanoparticles loaded with florfenicol were tested at six different concentrations and compared their efficacy against the bacterial strains. Our results demonstrated that all three clinical bacterial strains exhibited varying degrees of resistance to florfenicol. Interestingly, the use of polymeric nanoparticles loaded with florfenicol did not display enhanced antimicrobial activity compared to the free drug. Notably, the efficacy of the loaded nanoparticles did not significantly vary with different concentrations of active substance. This study highlights the importance of exploring novel therapeutic approaches to combat antimicrobial resistance. The use of polymeric nanoparticles loaded with florfenicol presents a promising avenue for overcoming resistance mechanisms and improving the efficacy of antimicrobial treatments both in human and veterinary medicine. Further investigations are needed to elucidate the underlying mechanisms and optimize the formulation of polymer nanoparticles for enhanced therapeutic outcomes in combating AMR