Salivary Flow Rate During Toothbrushing

PURPOSE To determine the salivary flow rate and subsequent dilution of toothpaste and assess the pH of oral fluids during toothbrushing with toothpastes of various pHs. MATERIALS AND METHODS The study was conducted as an in-vivo trial involving 30 healthy volunteers. The participants took part in a series of trials distributed over four appointments. After a screening check, in which the participants' stimulated and unstimulated salivary flow rate and buffering capacities were determined, four test series involving toothbrushing were conducted. Participants brushed their teeth using a manual toothbrush for 2 min: once without toothpaste and three times using toothpastes of varying pHs. The salivary flow rate and subsequent dilution of the toothpaste was determined. Additionally, the pH of the collected oral fluid was analysed. RESULTS Brushing teeth with toothpaste caused a statistically significant increase in salivary flow rate (median/IQR in ml/min) (Elmex Kariesschutz 3.29/1.36, Colgate Total Original 3.23/1.08, Elmex Sensitive Professional 3.18/1.39) when compared to brushing teeth using a manual toothbrush without toothpaste (1.85/0.78) (p < 0.05). The variation in pH of the oral fluid samples was dictated primarily by the pH of the toothpaste used. CONCLUSION The salivary flow rate when brushing using toothpaste was similar across all tested toothpastes, independent of pH, and had an average median of 3.23 ml/min. The dilution of 1 g of toothpaste during a standard toothbrushing procedure of 2 min is therefore approximately at a ratio of one part toothpaste to 6.5 parts saliva

Zahnerosionen im Zusammenhang mit gastroösophagealem Reflux: Ursache, Prävention und restaurative Therapie

Tooth Erosions Associated with Gastroesophageal Reflux: Cause, Prevention and Restorative Therapy Abstract. Dental erosions are caused by the recurring contact of solutions which are unsaturated in tooth minerals,ith hard tooth substances. This initially leads to softening and later to an irreversible loss of hard tooth substance. Erosion is observed particularly with excessive consumption of acidic foods (e.g. soft drinks or citrus fruits) but also in connection with gastrointestinal (gastroesophageal reflux disease) or psychosomatic diseases (anorexia nervosa or bulimia nervosa). The aim of this article is to define dental erosions, their causes, prevalence and consequences as well as possible preventive measures. Based on a clinical example of a patient with reflux-related erosions, a therapy option with direct composite tooth build-up is presente

Abrasive Enamel and Dentin Wear Resulting from Brushing with Toothpastes with Highly Discrepant Relative Enamel Abrasivity (REA) and Relative Dentin Abrasivity (RDA) Values

PURPOSE To investigate the absolute wear caused by toothpastes with highly discrepant REA (Relative Enamel Abrasivity) and RDA (Relative Dentin Abrasivity) values on both enamel and dentin: Candida Peppermint (CP; REA: 1; RDA: 42), Colgate Total Original (CTO; REA: 4; RDA: 100), Signal White System (SWS; REA: 8; RDA: 143), and Candida White Diamond (CWD; REA 244; RDA: 12). MATERIALS AND METHODS Eighty (80) bovine enamel samples and 80 dentin samples were divided into four groups each (n = 20) and investigated after a 6-h brushing procedure (21,600 cycles, 60 cycles/min, load of 2.5 N) with the four toothpastes. The abrasive enamel and dentin wear were registered using a contact profilometer. The median and interquartile range (IQR) of the abrasive enamel and dentin wear were calculated for each group. Pairwise comparisons were conducted using the Wilcoxon signed-rank exact test, and the p-value was adjusted according to Holm (statistical significance set at 0.05). RESULTS CWD led to the highest abrasive enamel wear (9.86 μm [5.77]). CTO caused the highest abrasive dentin wear (166.70 μm [69.90]), being statistically significantly higher than the wear for CP (54.20 μm [24.00]) and CWD (17.00 μm [7.80]) (p = 0.00001). The abrasive dentin wear for CWD was statistically significantly lower in comparison to all other groups (p = 0.00001). CONCLUSION Toothpastes with highly discrepant REA and RDA values presented statistically significantly different absolute wear on enamel and dentin. REA and RDA values should both be declared for every toothpaste

Supplementation of Energy Drinks with Green Tea Extract: Effect on In Vitro Abrasive/Erosive Dentin Wear

PURPOSE: To investigate the effect of the supplementation of energy drinks with green tea extract on abrasive and erosive dentin wear. MATERIALS AND METHODS: Six groups, each comprising 15 bovine dentin samples, were prepared, yielding a total of 90 samples. Erosion was performed by immersing the samples in Red Bull and Red Bull light with and without green tea extract. Tap water with and without green tea extract was used as the control groups. The samples were subjected to abrasive/erosive cycling for five days. The following cycling was performed daily: toothbrush abrasion (20 brushstrokes; 2.5 N); eight erosive cycles (2 min storage in the respective solutions); in between the erosive cycles, storage in artificial saliva (60 min) and again toothbrush abrasion (20 brushstrokes; 2.5 N). During the night, samples were again stored in artificial saliva. Abrasive/erosive dentin wear was measured using a stylus profilometer (µm, accuracy = 40 nm). The measured dentin loss results from the vertical position shift on the y-axis from base to final profile after the wear process in 2D. Pairwise comparisons between the groups were carried out using Wilcoxon signed-rank test. RESULTS: The following dentin wear (median [IQR]) was measured: Red Bull: 1.9 µm (0.5); Red Bull Light: 1.3 µm (0.3); Red Bull with green tea extract: 0.8 µm (0.3); Red Bull Light with green tea extract: 0.3 µm (0.5); Tap water with green tea extract: -0.2 µm (0.7); Tap water: -1.0 µm (1.2). The comparison of all tested groups to each other proved to be statistically significant (p < 0.05). CONCLUSION: The supplementation of energy drinks with green tea extract provide a protective effect against erosive/abrasive wear in vitro

Interplay Between the In-Vitro Cleaning Performance and Wear of Manual Toothbrushes

PURPOSE This in-vitro study deals with the question of whether the wear and tear of the manual toothbrush over a simulated timeframe up to 24 months has an effect on its cleaning performance. The purpose was to find indications as to whether and when a toothbrush needs to be replaced based on its cleaning performance. MATERIALS AND METHODS Models equipped with artificial teeth (coated with titanium dioxide) were brushed in-vitro using a brushing machine with clamped manual toothbrushes. The machine carried out even, horizontal brush strokes (120 brush strokes/min) for 1 min with a constant contact pressure of 2.5 N. The percentage of the area of titanium dioxide removed from the buccal, mesial and distal surfaces of the artificial teeth corresponded to the cleaning performance. The manual toothbrushes were used on bovine roots to simulate the wear and tear after 2, 4, 6, 12, 18, 24 months of use. The cleaning performance was re-evaluated after each simulated timepoint of wear. In addition, the brushes were photographed after each cycle. RESULTS An increase in the in-vitro cleaning performance of the toothbrush was observed up to 6 months of wear compared to the starting point. After that, the cleaning performance decreased somewhat, but always remained above the initial cleaning performance. CONCLUSION Based on the in-vitro cleaning performance after 24 months, the toothbrush would not have to be replaced. However, this in-vitro study cannot determine when a toothbrush should be replaced, because in-vivo it is also dependent on a variety of other factors such as fraying and microbial colonisation. Direct transfer of results from this study to everyday clinical practice is therefore difficult

Relative dentin and enamel abrasivity of charcoal toothpastes

Objectives: Very little data are known about charcoal toothpastes. The aim of this study was to counteract the missing data by determining the relative dentin abrasivity (RDA) and relative enamel abrasivity (REA) values of charcoal toothpastes. Methods: Radioactively charged dentin and enamel samples were randomly divided into groups of eight specimens. Each group was brushed with two of total 12 charcoal toothpaste slurries and with a standard abrasive with a known RDA and REA value. The measured radioactivity in counts per minute within the slurries corresponds to the amount of dentin or enamel abraded. RDA and REA values of the charcoal toothpastes were expressed relative to the known value of the standard slurry. Results: The RDA and REA values of the charcoal toothpastes have a broad range of 24-166 and 0-14, respectively. Conclusions: The RDA and REA values do not differ significantly from previously tested commercially available toothpastes. However, the lack of fluoride compounds in many of the investigated charcoal toothpastes can have a less beneficial effect for the consumers. It is very important to educate patients accordingly. Keywords: RDA; REA; abrasivity; activated charcoal; toothpaste

Influence of pretreatments on microtensile bond strength to eroded dentin using a universal adhesive in self-etch mode

Purpose To evaluate the influence of pretreatments on microtensile bond strength (μTBS) to eroded dentin when using a universal adhesive in self-etch mode. Materials and methods Forty-eight human molars were randomly assigned to six groups (G1–G6, n = 8) and ground down until dentin exposure. G2–G6 were eroded with citric acid (pH 2.6) for 10 × 2 min (within 12 h) on 5 consecutive days. G1 and G2 served as uneroded and eroded controls and were not pretreated. G3 and G4 were pretreated for 60 s with NaOCl (10% and 5%, respectively). G5 was airborne-particle abraded using Al2O3 for 15 s, and G6 was roughened with a diamond bur. The universal adhesive Scotchbond Universal was applied in self-etch mode. After composite build-up, μTBS was determined. Results Control group G1 (uneroded) showed higher μTBS than G2 (eroded). The μTBS (mean MPa ±SD) of G5 (airborne-particle abrasion; 11.6 ± 4.6) and G6 (bur-roughening; 11.0 ± 3.3) did not differ significantly from the μTBS of G1 (12.4 ± 4.2). Significantly higher μTBS compared to all other groups was observed in G3 (NaOCl 10%; 17.6 ± 7.6). Pretreatment with NaOCl 5% (G4) achieved significantly lower μTBS (3.5 ± 2.6) than the other groups, except for G2 (8.8 ± 2.0). Conclusions Pretreatment with 10% NaOCl, diamond bur roughening or airborne-particle abrasion can increase bond strength of the tested adhesive to eroded dentine. Pre-treatment with 5% NaOCl is not recommended

Effect of Toothbrush Bristle Stiffness and Brushing Force on Cleaning Efficacy

PURPOSE This study investigated the effect of toothbrush bristle stiffness and brushing force on the cleaning efficacy in vitro. MATERIALS AND METHODS Eighty bovine dentin samples were allocated to eight groups (n=10). Two custom-made toothbrushes of different bristle stiffness (soft and medium) were tested at four different brushing forces (1, 2, 3 and 4 N). Dentin samples were stained in black tea and brushed (60 strokes/min) for a total of 25 min in a brushing machine with an abrasive solution (RDA 67). Photographs were taken after 2 and 25 min of brushing time. Cleaning efficacy was measured planimetrically. RESULTS After 2 min of brushing, the soft-bristle toothbrush did not cause statistically significantly different cleaning efficacy at different brushing forces, while the medium-bristle toothbrush cleaned statistically significantly less efficaceously only at 1 N. Comparing the two different toothbrushes, higher cleaning efficacy was observed only at 1 N for the soft-bristle brush. At 25 min brushing time, the soft-bristle cleaned statistically significantly better at 4 N compared to 1 N and 2 N and at 3 N compared to 1 N. Using the medium-bristle, cleaning efficacy increased with increasing brushing force. After 25 min of brushing, no statistically significant difference was observed between the two different toothbrushes. CONCLUSION Irrespective the brushing force, the use of a soft or medium toothbrush results in comparable cleaning efficacy. At 2 min brushing time, increasing the brushing force does not increase the cleaning efficacy

Long-term protective effect of surface sealants against erosive wear by intrinsic and extrinsic acids

OBJECTIVE: To test sealants to prevent erosive tooth wear caused by extrinsic and intrinsic acids under long-term exposition. METHODS: 144 bovine enamel samples were randomly allocated to twelve groups (1-12). Samples of groups 1, 5 and 9 remained unsealed (positive controls), 2, 6 and 10 were sealed with Silicon Seal Nano Mix and 3, 7 and 11 with Seal&Protect. Groups 4, 8 and 12 were sealed with flowable composite (negative controls). Groups 1-4 were immersed in artificial saliva, 5-8 in hydrochloric acid and groups 9-12 in citric acid for 28 days, respectively. After 1, 2, 4, 7, 11, 14, 21 and 28 days, solutions were renewed and enamel wear was quantified by assignation of (32)P in the solutions. RESULTS: In all immersion solutions, lowest mineral loss was observed for the negative controls whilst highest loss was observed for unsealed positive controls. In artificial saliva and citric acid, the loss from samples sealed with Seal&Protect was not significantly different compared with negative controls whilst loss in groups sealed with Silicon Seal Nano Mix was significantly higher. In hydrochloric acid, loss from samples sealed with Seal&Protect was not different compared with that of negative controls up to 4 days. Except day 1, the mineral loss in the Seal&Protect group was significantly lower compared with that of the Silicon Seal Nano Mix group. CONCLUSION: The tested resin based surface sealant is able to significantly reduce the erosive demineralisation of enamel caused by hydrochloric and citric acid even under long-term exposition