Improving the Authorization of Microbial Biological Control Products (MBCP) in the European Union within the EU Green Deal Framework

Developing sustainable agriculture by identifying non-chemical alternative Plant Protection Products (PPP) is a cornerstone in achieving long-sought environmental friendliness. Despite significant legislative and political efforts to promote biocontrol solutions and Integrated Pest Management (IPM), the literature points out the disadvantages posed by European Union’s (EU) two-tier system for Microbial Biological Control Agents (MBCA) approval and subsequent Microbial Biological Control Products (MBCP) authorization by each EU Member State (MS). Despite the disadvantages, in a recent article, we showed that the EU had outcompeted the US and other countries in approved MBCA in the last decades; however, MBCP approval at the national level lags. Achieving the EU Green Deal’s aim set out in the ‘Farm to Fork Strategy’ to reduce the use and risk of pesticides by 50% by 2030 is difficult without developing viable alternatives. Why do we not have higher MBCP availability and usage in the EU? Is it the current legislation, its poor application, or some other factors? The current legislative framework stimulated MBCA approval. Thus, we compare MBCA approval and MBCP authorization procedure to evaluate if MBCP authorization is more difficult and thus causes a bottleneck. We find that requirements for MBCP authorization are unnecessarily more complex. We recommend simplifying the MBCP dossier requirements and making them as similar to MBCA as possible to accelerate the MBCP authorization in more EU MS to increase their availability and integration in agronomic crops’ pest management plans

Improving the Authorization of Microbial Biological Control Products (MBCP) in the European Union within the EU Green Deal Framework

Developing sustainable agriculture by identifying non-chemical alternative Plant Protection Products (PPP) is a cornerstone in achieving long-sought environmental friendliness. Despite significant legislative and political efforts to promote biocontrol solutions and Integrated Pest Management (IPM), the literature points out the disadvantages posed by European Union’s (EU) two-tier system for Microbial Biological Control Agents (MBCA) approval and subsequent Microbial Biological Control Products (MBCP) authorization by each EU Member State (MS). Despite the disadvantages, in a recent article, we showed that the EU had outcompeted the US and other countries in approved MBCA in the last decades; however, MBCP approval at the national level lags. Achieving the EU Green Deal’s aim set out in the ‘Farm to Fork Strategy’ to reduce the use and risk of pesticides by 50% by 2030 is difficult without developing viable alternatives. Why do we not have higher MBCP availability and usage in the EU? Is it the current legislation, its poor application, or some other factors? The current legislative framework stimulated MBCA approval. Thus, we compare MBCA approval and MBCP authorization procedure to evaluate if MBCP authorization is more difficult and thus causes a bottleneck. We find that requirements for MBCP authorization are unnecessarily more complex. We recommend simplifying the MBCP dossier requirements and making them as similar to MBCA as possible to accelerate the MBCP authorization in more EU MS to increase their availability and integration in agronomic crops’ pest management plans

Greener European Agriculture? Evaluating EU Member States’ Transition Efforts to Integrated Pest Management through Their National Action Plans

Integrated pest management (IPM) is among the most promising approaches for transforming today’s agronomical practices toward sustainable and environmentally friendly agriculture. Aiming to become a global environmental leader, in 2009, the European Union (EU) embraced the idea of making IPM practices ubiquitously used by 2014 in all EU Member States (EU MSs). Through Directive 2009/128/EC (the Sustainable Use Directive (SUD), the EU required EU MSs to structure their transformative measures in National Action Plans (NAPs) in a comprehensive effort. These documents have a fundamental role in orienting the plans and activities of national stakeholders, such as agronomists, researchers, and local and national-level institutions. We analyze and compare the second-generation NAPs (2019–2022) of 10 EU MSs to assess their strengths and weakness and their modifications from the first generation of NAPs (2009). We advance several recommendations on how to make them more valuable instruments in structuring activities towards achieving the goals of the SUD

Towards Sustainable European Agriculture? Assessing the EU’s Progress in Limiting the Negative Ecological Effects of Agriculture on Aquatic Environments

The degradation of terrestrial and aquatic environments has significant adverse effects on biodiversity and environmental sustainability. The ever-increasing population and constant economic growth strain various ecosystems’ resistance and resilience. An important factor that negatively influences terrestrial and aquatic ecosystems is the use of products used in crop management. In this article, we analyze the pesticide-related European Directives and the Nationals Action Plans (NAP) regarding the sustainable use of pesticides and other documents evaluating the NAPs implementation. We assess the first and second-generation NAPs of nine EU Member States (MS)’ to evaluate if we can identify a significant shift in managing the adverse effects of pesticide use on aquatic environments. Furthermore, we evaluate the degree to which these NAPs are synergic with the EU’s strategic approach to soil protection, aquatic environments, and biodiversity

Phenological and Environmental Factors’ Impact on Secondary Metabolites in Medicinal Plant <i>Cotinus coggygria</i> Scop.

Cotinus coggygria Scop. (smoketree) is a phytotherapeutically valuable shrub growing in specific areas in many Eurasian countries. Exploring the intrinsic and extrinsic (abiotic) factors that modulate its secondary metabolism has fundamental and applicative importance. Three smoketree plants from the same population were studied for a period of 4.5 months. Their extracts were characterized using LC–MS/MS, HPLC–UV–VIS–DAD and colorimetric assays to determine the chemical composition and antioxidant potential. Multivariate analysis was applied to correlate the metabolomic data with registered habitat variables and phenological stages. The identified and quantified compounds belonged to the flavonoids (myricetin-3-O-galactoside, myricitrin) and hydrolysable tannins groups (pentagalloyl glucose, methyl gallate, methyl digallate I). Phenolic compounds and tannins were synthesized abundantly in the flowering and fruit stages, whereas flavonoids and triterpenes accumulated during senescence. The antioxidant activities varied between detection methods, samplings and individuals and were only punctually correlated with the compound contents in certain phenological stages. Based on the HCAbp analysis, the samples clustered under four groups, according to their metabolic profile. The CCA analysis revealed that during the reproductive stages (flower, fruit or seed), the secondary metabolism of the plants’ leaves is sensitive to the action of abiotic factors, while in senescence, the metabolic content is according to the phenological phase. This study provides a first attempt at understanding the interplay between the habitat and the metabolome of smoketree

The Impact of Acute Low-Dose Gamma Irradiation on Biomass Accumulation and Secondary Metabolites Production in <i>Cotinus coggygria</i> Scop. and <i>Fragaria × ananassa</i> Duch. Red Callus Cultures

Cotinus coggygria Scop. (smoketree) and Fragaria × ananassa Duch. (strawberry) are two industrially important species due to their composition in bioactive compounds. In this study, we investigated the effects of acute low-dose gamma irradiation (15, 20, 25, 30, 35 and 40 Gy) on two red callus cultures established in smoketree and strawberry. The biomass production, dry weight, content of phenols, flavonoids, monomeric anthocyanins’, index of anthocyanins polymerization and antioxidant activity were evaluated. For the smoketree callus, a negative correlation between irradiation doses and callus biomass accumulation was observed. For the strawberry callus, irradiation did not significantly affect the accumulation of the biomass. An increased dry weight was observed in irradiated smoketree callus, while for treated strawberry callus, a decrease was recorded. Irradiation with 30 Gy was stimulative for polyphenols’ accumulation in both cultures; however, the increase was significant only in the strawberry callus. The flavonoids increased in the 30 Gy strawberry variants, while it significantly decreased in smoketree callus irradiated with 35 and 40 Gy. In irradiated strawberry callus, except for the 25 Gy variant (1.65 ± 0.4 mg C-3-GE/g DW), all treatments caused an increase in anthocyanins’ accumulation. In smoketree, except for the 15 Gy variant (2.14 ± 0.66 mg C-3-GE/g DW), the irradiation determined an increase in anthocyanins synthesis, with the highest value being seen in the 20 Gy variant (2.8 ± 0.94 mg C-3-GE/g DW). According to UPLC-HRMS investigations, an unidentified compound increased by 99% at the 30 Gy dose in strawberry callus, while in smoketree, maslinic acid increased by 51% after irradiation with 40 Gy. The results of this study showed, for the first time, the differential response of two performant callus cultures to low-dose gamma irradiation, a biotechnological method that can be used to stimulate the synthesis of important flavonoids and triterpenes

Population and Conservation Status of Buxbaumia viridis (DC.) Moug. &amp; Nestl. in Romania

The field survey made in the last 20 years revealed that large areas of Romania&rsquo;s territory are still unexplored concerning moss distribution. The long-term research in natural and mature spruce forest habitats of this country shows that many sites are well protected, a status that is also confirmed by reports of Buxbaumia viridis. However, many other sites where this species was identified still lack legal protection. We also generated a potential distribution of the species based on an ensemble model, useful in guiding extensive field surveys and also management and conservation measures. In a country where the volume of wood cut by illegal logging is larger than the legal one, according to official data, it is very important that all habitats for B. viridis be included in protected areas. Our paper brings important data to aid in this goal