Intrinsic bioremediation of landfills interim report

Intrinsic bioremediation is a risk management option that relies on natural biological and physical processes to contain the spread of contamination from a source. Evidence is presented in this report that intrinsic bioremediation is occurring at the Sanitary Landfill is fundamental to support incorportion into a Corrective Action Plan (CAP)

Direct immunofluorescence and enzyme-linked immunosorbent assays for evaluating chlorinated hydrocarbon degrading bacteria

Immunological procedures were developed to enumerate chlorinated hydrocarbon degrading bacteria. Polyclonal antibodies (Pabs) were produced by immunizing New Zealand white rabbits against 18 contaminant-degrading bacteria. These included methanotrophic and chlorobenzene (CB) degrading species. An enzyme-linked immunosorbent assay (ELISA) was used to test for specificity and sensitivity of the Pabs. Direct fluorescent antibodies (DFAs) were developed with these Pabs against select methanotrophic bacteria isolated from a trichloroethylene (TCE) contaminated landfill at the Savannah River Site (SRS) and cultures from the American Type Culture Collection (ATCC). Analysis of cross reactivity testing data showed some of the Pabs to be group specific while others were species specific. The threshold of sensitivity for the ELISA is 105 bacteria cells/ml. The DFA can detect as few as one bacterium per ml after concentration. Results from the DFA and ELISA techniques for enumeration of methanotrophic bacteria in groundwater were higher but not significantly different (P < 0.05) compared to indirect microbiological techniques such as MPN. These methods provide useful information on in situ community structure and function for bioremediation applications within 1--4 hours of sampling

Aeromomas in a thermally stressed lake. [Survival of fish pathogenic bacteria in Par Pond]

Non

Bioremediation of contaminated groundwater

Disclosed is an apparatus and method for in situ remediation of contaminated subsurface soil or groundwater contaminated by chlorinated hydrocarbons. A nutrient fluid (NF) is selected to simulated the growth and reproduction of indigenous subsurface microorganisms capable of degrading the contaminants; an oxygenated fluid (OF) is selected to create an aerobic environment with anaerobic pockets. NF is injected periodically while OF is injected continuously and both are extracted so that both are drawn across the plume. NF stimulates microbial colony growth; withholding it periodically forces the larger, healthy colony of microbes to degrade the contaminants. Treatment is continued until the subsurface concentration of contaminants is acceptable. NF can be methane and OF be air, for stimulating production of methanotrophs to break down chlorohydrocarbons, especially TCE and tetrachloroethylene

Microbial Physiology of Subsurface Aquifers at SRS

This report describes a rapid screening procedure used to test the enzymatic capabilities of bacterial consortia in groundwater from well clusters P-24, P-28, and P-29

Characterization and reclamation assessment for the central shops diesel storage facility at Savannah River Site

The contamination of subsurface terrestrial environments by organic contaminants is a global phenomenon. The remediation of such environments requires innovative assessment techniques and strategies for successful cleanups. Using innovative approaches, the central Shops Diesel Storage Facility at the Savannah River Site (SRS) was characterized to determine the extent of subsurface diesel fuel contamination. Effective bioremediation techniques for cleaning up of the contaminant plume were established

Microbial Condition of Water Samples from Foreign Fuel Storage Facilities

In order to assess the microbial condition of foreign nuclear fuel storage facilities, fourteen different water samples were received from facilities outside the United States that have sent spent nuclear fuel to SRS for wet storage. Each water sample was analyzed for microbial content and activity as determined by total bacteria, viable aerobic bacteria, viable anaerobic bacteria, viable sulfate- reducing bacteria, viable acid-producing bacteria and enzyme diversity. The results for each water sample were then compared to other foreign samples and to data from the receiving basin for off- site fuel (RBOF) at SRS

Environmental Transport Division. 1980 report

Aquatic, atmospheric, and terrestrial studies and instrumentation developments are described in a series of articles. More details about specific studies are given in publications listed at the end of the report

Isolation of legionnaires' disease bacteria from non-epidemic related habitats

Continuous centrifugation of large volumes of water from natural southeastern lakes allowed quantitative screening for Legionnaires' disease bacteria (LDB) by direct immunofluorescent staining. Presumptively positive samples were injected intraperitoneally into guinea pigs and the LDB were isolated and identified by their morphological, cultural, physiological, and serological characteristics

Distribution of Aeromonas hydrophila in a South Carolina cooling reservoir. [Aeromonas hydrophila]

Par Pond is a thermally enriched monomictic southeastern lake which receives heated effluent from a production nuclear reactor. Fish populations in the lake have lesions of epizooty from which Aeromonas spp. are readily isolated. Distribution and population densities of Aeromonas in the water column were measured along an oxygen and temperature gradient. Greater population densities of Aeromonas occurred below the oxygen chemocline when the lake was stratified. Survival of A. hydrophila under in situ conditions in both epilimnetic and hypolimnetic waters was determined using polycarbonate membrane diffusion chambers, during two separate reactor operating conditions. Survival levels of pure cultures of A. hydrophila corresponded to the distribution patterns of the naturally occurring Aeromonas-like populations. The greater survival of A. hydrophila below the chemocline when the reactor was in full operation suggests that the fish populations may be exposed to Aeromonas for a longer period of time than when the reactor is not operating