In this paper, three methods of sterilisation are compared to determine their usability in nanobubbledispersion sterilisation: filtration, thermal sterilisation and sonication (in twosystems: using a sonotrodeand sonication bath). Nanobubble dispersions are most commonly generated in non-sterile systemswhich precludes them from use in most biological research. As a result of this study, filtration waschosen as the best method for nanobubble sterilisation