Detection of Genome Content of Pseudomonas Aeruginosa Biofilm Formation and Resistance to Some Disinfectants and Antibiotics

This study have been includedfortyisolates ofthe bacteriumPseudomonas aeruginosaisolatedfrom Department ofbiology /College of Science /University of Mustansiriya from different clinical specimens itwas diagnosisbased on themethodsof microscopic,culturalandbiochemicalandfinal diagnosisusingVitek 2system . The ability of the isolates to produce biofilmwas tested usingCongo Red Agar Method (CRA).The resultshowed that(12)isolated (30%) are biofilm producer. Antibiotic sensitivity test have been done multiple drug resistance therevealed showed thatisolates had moreoverallclinical isolates ofP. aeruginosa 100%) )were resistantto Piperacillin, Amoxicillin / Clavulanic acid, Cephalexin, Ceftriaxone and Ceftazidime and while the isolates gradually were resisttoother antibiotics untilshowedlessresistantto theImipenem.( 16.7%)In addition, the minimum inhibitory concentration (MICs) for P. aeruginosa isolateswere determinedto five antibiotics included ( Amikacin, Ciprofloxacin Ceftriaxone, Ceftazidime and Imipenem) and three types of disinfectants (Povidin - Iodine, Chloroxylenol, Formalin) . Bacterial isolates under this study were showed high resistance to both Ceftriaxone and Amikacin. The rang of (MICs) Values was between (16-512) µg / mL. the results showed Values of the (MICs) of disinfectant formalin was low compared otherdisinfectants in this study. Since rang values(MICs) between (512-2000) µg / mL, (512-3500) µg / mL for Dettol and (1024-4000) µg / mL for Iodine solution. The study also showed that sensitivity percentage increased with increasing of disinfectant concentration. All isolates showed negative results for of Metallo-β-lactamase by EDTA method and PCR showed negative results for both of blaSHVand blaTEM genotype , While isolates showed positive results for blaCTX-M