Lengthening single-stem rotation improves biomass yield and water use efficiency in black poplar genotype multi-stem rotation coppice plantations

Poplar short rotation coppice (SRC) plantations have great potential for supplying environmentally friendly bio-based industries. However, little research has focussed on the linkages between SRC management regimes and the consumption of water for biomass production in the Mediterranean environment. Therefore, we compared six hybrid clones and four native black poplar genotypes with an aim to examine how two different lengthening periods (3 vs. 5 years) of single-stem rotation affected growth performance in the following three years of multi-stem rotation coppice. To achieve this goal, we assessed the aboveground dry biomass production and variation in water use efficiency (WUE) of the genotypes annually. A longer single-stem rotation increased biomass productivity and WUE in the multi-stem rotation of the native black poplar, rather than that of the hybrid genotypes. In contrast, biomass and WUE performances did not diverge between the native and hybrid genotypes under the shorter single-stem phase. These findings underline the importance of lengthening the rotation of single-stem SRC plantations in hot and dry Mediterranean climates. Native black poplar genotypes managed in SRC should be strongly considered as environmentally compatible genetic resources both in protected areas and in areas where water supply constrains biomass production

New technologies sustainability: monitoring and evaluation of results of interventions for the promotion of cultural heritage and the human landscape

The relationship between the development of technologies and the history of the cultural and agricultural landscape is linked to the concepts of "cultural landscape", understood as a space in continuous construction that changes with the change of individual, collective, social and cultural relationships of the inhabitants of the territory, or of the "cultural inhabitants", citizens who are producers of culture, rather than users. A vision of the "future as an open place" emerges, understood as a place of usability and sharing of all human, material and immaterial productions.Technologies, within a similar perspective, are presented as the historical evolution of téchne, whose degree of development today allows an extension of the level of human action.This study, in agreement with the scientific literature based on the use of recently developed digital models, demonstrates that the mainly agricultural territory of Basilicata, historically the site of complex social relations, has created a traditional rural society in which the concept of neighborhood and the spatial connotation also had the symbolic value of sharing knowledge and practices, relationships based on inclusiveness and sustainability. The diffusion of 5G technology is generating important cultural transformations. What used to be the neighborhood community in Matera (IT) - also following the activities launched with the CTEMT project and the social consequences of the Covid-19 pandemic - is now becoming a virtual community for sharing knowledge and practices , beliefs and values, including the use and management of cultural heritage, which takes place through the network, and therefore using applications that promote a transformative intervention of the landscape, such as to make it functional to human needs, and, at the same time, sustainable with respect to the perpetuation of ecosystem relationships.The diffusion of 5G technology, is generating important cultural transformations. What in the past was, in Matera (IT), the neighbourhood community - also as a result of the activities launched with the CTEMT project and the social consequences of the Covid-19 pandemic - now becomes a virtual community, sharing knowledge and practices, beliefs and values, including the use and management of cultural heritage, occurs through the network with the use of applications that promote accessibility and sustainability in both the urban and agricultural landscape. As argued by the International Union for Conservation of Nature (IUCN), the attention to the dynamic conservation of the landscape should not be placed so much to the "culture itself" or to the "nature itself" but rather to the relationship between these two dynamic components has been established, but also from the holistic mentioned many times, attentive to the values of identity and comforted by the knowledge and decoding of the intangible heritage, from which we deduce the active role, shared social behaviours, the mechanisms of transmission of knowledge and transgenerational awareness also thanks to the complex and fascinating universe of uses, traditions, rituals and rites that are an important tool of conscious management of the landscape and its culture. The conscious use of artificial intelligence is the concretion of the virtuous relationship between Humanism and technologies. For the biodiversity it is a support to the recognition of the species, in particular of the native ones, and it allows people to recognize themselves culturally and find into the biodiversity a collective and cultural belonging to the community and to the landscape. Therefore, thanks to the use of new technologies biodiversity becomes an historical-anthropological archive of knowledge and practices of a territory, and new technologies a powerful tool for the conservation of the cultural heritage

NH3 Emissions From Treated Buffalo Manure Application In Mediterranean climate And Comparison To ALFAM Model

Ammonia volatilization is widely recognized as one of the major environmental European problems, due to the increase in livestock farming activities. As a consequence, accurate ammonia assessment is needed in order to control ammonia emissions and to update national emission inventories. Besides some uncertainties still related to the measurement methods, another important issue is the necessity of investigating a different kind of fertilizers. In the last few years, considerable attention has been paid to many manure treatments prior to field application. This study aims to assess ammonia emissions from the field application of separated buffalo manure digestate in the Mediterranean climate, in order to improve the emission inventory for this animal species, reared mostly in South Italy. Two measuring methods were used: wind tunnel (WT) and Integrated Horizontal flux (IHF). Moreover, ammonia emission measured were compared to those obtained running the statistical regression model ALFAM. This model based on Michaelis-Menten type equation is often used to predict cumulative ammonia loss and since it is based on a significant dataset is useful to discuss the effectiveness of the emission measured. The total ammonia losses measured in 7 days were 26.39 and 49.24 kg N ha-1, for WT and IHF, respectively. Although the predicted total emissions were 40.99 and 36.56 kg N ha-1, for IHF and WT, respectively, it is possible to observe the good accordance of the ALFAM model with the temporal pattern of both methods

Ammonia Emission Assessment After Buffalo Manure And Digestate Application

Quite recently, considerable attention has been paid to the effect of anaerobic digestion on ammonia emissions from digestate spreading in the field, due to the growing interest in NH3 emission monitoring. Unfortunately, there are still some different studies finding about the effect of anaerobic digestion on ammonia emissions. Thus, more research into this topic is still necessary before obtaining a definitive answer to the increment or not in emission. For this purpose, this paper proposes comparison study between ammonia emissions from buffalo raw (Farmyard) manure and digestate on bare soil under Mediterranean climate, using the wind tunnel equipped with acid traps, to assess the ammonia emission fluxes. The sampling campaign, in three replicates, lasted each time, for 6 days to ensure that most of the ammonia has been emitted before the end of each campaign. The results obtained indicate that a diurnal correlation between emission and external temperature occurs, especially during the first days. Specifically for both fertilizers, ammonia volatilization increased with air temperature raising. Overall, the total digestate cumulative NH3 emission is 54% higher than raw manure emission. This is certainly due to the Total NH4+-N rate, which was 55,8kg ha-1 for the raw manure and 107 kg ha-1 for the digestate, around 1,9 times higher for the digestate TAN content. Finding suggests the need for adjusting digestate application rate based on TAN content, in order to reduce the impact on the environment

Durum Wheat in Conventional and Organic Farming: Yield Amount and Pasta Quality in Southern Italy

Five durum wheat cultivars were grown in a Mediterranean area (Southern Italy) under conventional and organic farming with the aim to evaluate agronomic, technological, sensory, and sanitary quality of grains and pasta. The cultivar Matt produced the best pasta quality under conventional cropping system, while the quality parameters evaluated were unsatisfactory under organic farming. The cultivar Saragolla showed the best yield amount and pasta quality in all the experimental conditions, thus proving to be the cultivar more adapt to organic farming. In all the tested experimental conditions, nivalenol (NIV) and deoxynivalenol (DON) occurrence was very low and the other mycotoxins evaluated were completely absent. These data confirm the low risk of mycotoxin contamination in the Mediterranean climate conditions. Finally, it has been possible to produce high-quality pasta in Southern Italy from durum wheat grown both in conventional and organic farming

Investigating Lead Bioavailability in a Former Shooting Range by Soil Microanalyses and Earthworms Tests

Shooting ranges are among the major anthropogenic sources of Pb contamination in soils worldwide. Once they have reached the soil, bullet residues can have different fates according to the characteristics of the soil environment, leading to the formation of different Pb weathering products whose stability is crucial for Pb accessibility to soil biota. In this study, Pb availability in a former polluted shooting range was investigated with a combination of conventional soil analyses, X-ray microanalyses and assays with the bio-indicator earthworm Eisenia andrei. Chemical extractions evidenced a rather low mobility of soil Pb, while micro-X-ray fluorescence spectroscopy (mu XRF) and scanning electron microscopy coupled with microanalysis (SEM-EDX) showed the formation of a weathering crust around Pb-containing bullet slivers dispersed within the soil. Such crusts consisted of a mixture of orthophosphates, including the highly insoluble Cl-pyromorphite. Furthermore, no acute toxicity effects and low Pb concentration values were measured in earthworm tissues (94.9 mg kg(-1)) and coelom fluids (794 mu g L-1) after 28 days of exposure to the polluted soil. These results allow us to assume that most of the Pb in the shooting range soil underwent stabilization processes promoted by phosphatic fertilization. The soil was in fact used for agriculture after being dismissed for firing activities. Such a combined approach can be applied to study Pb bioavailability in other shooting ranges or, more generally, in soils heavily polluted with P

Biotechnological synthesis of succinic acid by actinobacillus succinogenes by exploitation of lignocellulosic biomass

Succinic acid is increasingly used in pharmaceutical industries, for the production of additives in food industries, in agriculture and in refinery processes as a precursor of many chemical compounds among which the most important is the succinate salt. It is also used as an ion chelator and surfactant, and for the biochemicals production. Currently, succinic acid is mainly produced through chemical petroleum-based processes, usually from n-butane using maleic anhydride. However, the use of petrochemical feedstocks raises serious environmental problems, due to the higher values of temperature and pressure required. The biotechnological production of succinic acid by microbial conversion of lignocellulosic biomass is attracting growing interest due to the environmental and economic advantages offered. This research is focused on the exploitation of Arundo donax (Giant reed) as a source of lignocellulosic biomass. Arundo donax is a perennial crop particularly suitable for energy production, as it offers high yields per hectare, even in partially fertile or polluted soils, not used for agriculture. Hydrolyzate of Arundo donax will be used as growth media for the Actinobacillus succinogenes 130Z, a bacterium typically found in the bovine rumen, that is recognized as one of the most promising for the biotechnological production of succinic acid, as it is able to produce higher concentrations of succinic acid. The experimental analysis is carried out to optimize the production of succinic acid taking into account the effect of the most critical parameters of the process (microbial biomass, pH, reducing sugars, volatile fatty acids, and succinic acid). Tests have shown that in 48h the sugars are completely biodegraded with a total production of bio-succinic acid of 5.9 g for 9.1 g of reducing sugars, an hourly production 0.12 g h-1 with a yield equal to 65%

Linking phytotechnologies to bioeconomy; varietal screening of high biomass and energy crops for phytoremediation of Cr and Cu contaminated soils

Enerbiochem was a project devoted to study new strategies of industrial valorisation of high biomass crops grown on brownfields or contaminated soils not suitable for food production. Chromium and copper accumulation and toxicity were examined in different species of agronomic interest. Cultivars of Brassica carinata A. Braun (7), Brassica juncea (L.) Czern. (4), Brassica napus L. (4), Raphanus sativus L. (4), inbred lines of Helianthus annuus L. (6) and cultivars of Nicotiana tabacum L. (3) were screened for the best genetic materials to be used with the aims: i) to produce the highest biomass in contaminated soils; and ii) possibly to phytoremediate them. Cr and Cu accumulation in shoots were evaluated on 16 days old plants grown for additional 5 days in the presence of either Cr (60 μM) or Cu (2 μM) in hydroponic. They were characterised for Cr and Cu concentrations in roots and shoots, shoot biomass, and total chlorophyll as well. Shoot biomass was significantly lower in Brassica species than in R. sativus, H. annuus and N. tabacum under Cr treatments. On the contrary, under Cu treatments, N. tabacum produced the lowest biomass in respect to other species. Potentially toxic element concentrations varied among genetic material and some genetic material resulted less affected (higher chlorophyll content and shoot biomass) even under higher Cu or Cr concentrations in shoot. Potential candidates within each species, to be used for coupling phytoremediation and biomass production on slightly Cr-Cu potentially contaminated soils are listed

Comparative assessment of autochthonous bacterial and fungal communities and microbial biomarkers of polluted agricultural soils of the Terra dei Fuochi

Organic and inorganic xenobiotic compounds can affect the potential ecological function of the soil, altering its biodiversity. Therefore, the response of microbial communities to environmental pollution is a critical issue in soil ecology. Here, a high-throughput sequencing approach was used to investigate the indigenous bacterial and fungal community structure as well as the impact of pollutants on their diversity and richness in contaminated and noncontaminated soils of a National Interest Priority Site of Campania Region (Italy) called “Terra dei Fuochi”. The microbial populations shifted in the polluted soils via their mechanism of adaptation to contamination, establishing a new balance among prokaryotic and eukaryotic populations. Statistical analyses showed that the indigenous microbial communities were most strongly affected by contamination rather than by site of origin. Overabundant taxa and Actinobacteria were identified as sensitive biomarkers for assessing soil pollution and could provide general information on the health of the environment. This study has important implications for microbial ecology in contaminated environments, increasing our knowledge of the capacity of natural ecosystems to develop microbiota adapted to polluted soil in sites with high agricultural potential and providing a possible approach for modeling pollution indicators for bioremediation purposes

Compost and microbial biostimulant applications improve plant growth and soil biological fertility of a grass-based phytostabilization system

In this work, a grass-based phytoremediation system integrated with an organic amendment and biostimulants was evaluated for remediating contaminated sites. Plant growth and biological fertility were monitored to assess the efficacy of a vegetative cap used as a safety measure to reduce sanitary and environmental risks of industrially contaminated soils and soil-washing sludges. Both matrices were potentially contaminated with Pb and Zn with an ecological risk index from low to moderate. According to potentially toxic elements (PTEs) bioaccessibility tests, the exposure to the released fine particulate matter may cause serious risks to human beings, in particular to children. The grass mixture was well adapted to both the substrates and a low PTEs mobility was detected, thus, reducing the leaching risk to ground water sources. Compost addition augmented significantly nitrogenase reductase (nifH) and ammonia monooxygenase (amoA) gene expression abundance in both substrates. Furthermore, a positive interaction between compost fertilization and a Trichoderma-based biostimulant inoculation was recorded in sludges resulting in a significant stimulation of nitrogen-fixing and ammonia-oxidizing bacteria. The application of compost and biostimulant increased soil fertility and plant growth. Furthermore, there was a slight reduction in PTE bioaccessibility, thus, improving the efficiency of the phytostabilization, limiting the resuspension and dispersion of the health-risk soil particulate