Análisis de traslocaciones recíprocas en diferentes poblaciones de centeno, Secale Cereale L

Tesis Univ. Complutense de Madrid.Depto. de Genética, Fisiología y MicrobiologíaFac. de Ciencias BiológicasTRUEProQuestpu

Molecular diversity and genetic relationships in Secale

The objective of this study was to quantify the molecular diversity and to determine the genetic relationships among Secale spp. and among cultivars of Secale cereale using RAPDs, ISSRs and sequence analysis of six exons of ScMATE1 gene. Thirteen ryes (cultivated and wild) were genotyped using 21 RAPD and 16 ISSR primers. A total of 435 markers (242 RAPDs and 193 ISSRs) were obtained, with 293 being polymorphic (146 RAPDs and 147 ISSRs). Two RAPD and nine ISSR primers generated more than 80% of polymorphism. The ISSR markers were more polymorphic and informative than RAPDs. Further, 69% of the ISSR primers selected achieved at least 70% of DNA polymorphism. The study of six exons of the ScMATE1 gene also demonstrated a high genetic variability that subsists in Secale genus. One difference observed in exon 1 sequences from S. vavilovii seems to be correlated with Al sensitivity in this species. The genetic relationships obtained using RAPDs, ISSRs and exons of ScMATE1 gene were similar. S. ancestrale, S. kuprijanovii and S. cereale were grouped in the same cluster and S. segetale was in another cluster. S. vavilovii showed evidences of not being clearly an isolate species and having great intraspecific differences

Del color de los ojos al interior del genoma. Nuevas tecnologías aplicadas a la educación: una experiencia en la enseñanza de la Genética

Depto. de Genética, Fisiología y MicrobiologíaFac. de Ciencias BiológicasFALSEsubmitte

Herramientas de aprendizaje para estudiantes de secundaria: Aplicación de la Genética a una situación real

Depto. de Genética, Fisiología y MicrobiologíaFac. de Ciencias BiológicasFALSEsubmitte

Herramientas de aprendizaje para estudiantes de secundaria en el campo de la Genética

Se ha diseñado una actividad: la caracterización molecular de la mutación de un gen que modifica el color de los ojos en Drosophila melanogaster, partiendo de un carácter morfológico, el color de los ojos, se obtendrá la secuencia del gen responsable y su localización en el genoma de la especie. Se pretende desarrollar una actividad práctica que permita a los alumnos de segundo ciclo de la ESO comprender la genética y la genómica y cómo estos conocimientos se pueden aplicar a distintas áreas: salud, biotecnología o impacto ambiental

Biochemical, physiological and genetic analysis of aluminum tolerance of different rye species

Aluminum (Al) toxicity is the major limitation for crop productivity in acid soils which are widespread all overthe world. Plant species differ in their responses to this abiotic stress having developed resistance mechanisms todetoxify and tolerate Al both internally and externally. Rye (Secale cerealeL.) is one of the most Al-tolerant cerealwith a valuable genetic background for breeding purposes. Wild relatives (Secalespp.) have great importanceonce they can provide new sources of genes related to this trait. Different cellular disorders possibly related to Altolerance/toxicity were observed through histochemical root staining methods in cultivated and wild ryes and acorrelation was found. Moreover, expression studies of seven candidate Al-tolerance genes (ScALMT1,ScMATE2,ScSTOP1,ScMDH1,ScMDH2,ScCu/ZnSODandScMnSOD) were performed in roots and shoots offive wild ryes.All genes seems to have an active contribution on Al resistance mechanisms of these ryes, however,ScALMT1andScMATE2genes clearly have a key role in the Al-tolerance increment. Al tolerance inSecalegenus seems tobe a genetically complex trait where different resistance mechanisms coexist, due to several genes whose cu-mulative effects improves the ability to withstand Al phytotoxicit

Characterization, genetic diversity, phylogenetic relationships, and expression of the aluminum tolerance MATE1 gene in Secale species

Aluminum (Al) is the main limiting factor for crop production in acidic soils. Efflux of organic acids is one of the mechanisms that determine Al-tolerance and Al-activated citrate transporter (multidrug and toxic compound extrusion) MATE1 gene is involved in different species. The contribution of the rye MATE1 gene (ScMATE1) depends on the rye (Secale cereale L.) cultivars and the crosses analyzed; there is no information about different rye species. The cDNA sequences, phylogenetic relationships, Al-tolerance, citrate exudation and expression of ScMATE1 gene were analyzed in several cultivars and wild species/subspecies of the Secalegenus. Genotypes highly tolerant to Al were found within this genus. For the first time, sequences of the cDNA of the ScMATE1 gene were isolated and characterized in wild ryes. At least two copies of this gene were found likely to be related to Al-tolerance. The sequence comparison of 13 exons of ScMATE1 revealed variability between species, but also inter- and intra-cultivars. Variations were found in the Al-induced expression of ScMATE1 gene, as well as its contribution to Al-tolerance. The pattern of citrate exudation was inducible in most of the species/subspecies studied and constitutive in few. The phylogenetic analysis indicated that ScMATE1 is orthologue of two genes (HvMATE1 and TaMATE1) involved in the Al stress response in barley and wheat, respectively, but not orthologue of SbMATE, implicated in Al-tolerance in sorghum. ScMATE1 is involved in the response to Al stress in ryes, but its contribution to Al-tolerance is complex, and like in other species, there are tolerant and sensitive alleles in the different cultivars and species studied