Diuretic Activity of the Aqueous Extract Leaves of Ficus glumosa

Experiments were carried out to validate the use of F. glumosa extract as a diuretic in the treatment of hypertension as claimed by traditional healers. The experiments were performed under the same conditions with two synthetic pharmacological diuretics considered as check (Furosemide and Amiloride hydrochlorothiazide). The aqueous extract leaves of F. glumosa accelerated the elimination of overloaded fluid. At the maximum of diuretic response, urinary osmolarity decreased significantly when compared with controls. The single dose treatment of the aqueous extract leaves of F. glumosa has significantly increased urine volume 24 h after administration of the extract. The stability of aldosterone level, the absence of correlation with the plasma levels of sodium, and the increased clearance of free water in the animals receiving the extract show that increased diuresis and natriuresis moderate elevation are tubular in origin. The increase in Na+, K+, and Cl− induced by the extract caused alkalinization of the urine and showed a strong inhibitory effect of carbonic anhydrase and saluretic. These effects were mainly observed at the dose of 375 mg/kg. These observations confirm the traditional use in the treatment of hypertension and support the importance of the conservation of local knowledge as well as the conservation of Cameroonian biodiversity

Tapinanthus dodoneifolius leaf inhibits the activity of carbohydrate digesting enzymes and improves the insulin resistance induced in rats by dexamethasone

Background: Tapinanthus dodoneifolius is a plant used in traditional African medicine to treat diabetes mellitus. This study aimed to evaluate the preventive antidiabetic potential of the aqueous extract of T. dodoneifolius leaves (AETD) in insulin resistant rats. Methods: A quantitative phytochemical study of AETD was carried out to determine the contents of total phenols, tannins, flavonoids, and saponins. AETD was tested in vitro on the activity of α-amylase and α-glucosidase enzymes. Insulin resistance was induced for 10 days by daily subcutaneous injection of dexamethasone (1 mg/kg). One hour before, the rats were divided into 5 groups and treated as follows: group 1 received distilled water (10 mL/kg); group 2 received metformin (40 mg/kg), and groups 3, 4, and 5 were treated with AETD (125, 250, and 500 mg/kg). Body weight, blood sugar, food and water consumption, serum insulin level, lipid profile, and oxidative status were assessed. One-way analysis of variance followed by Turkey's post-test and two-way analysis followed by Bonferroni's post-test were used to analyze univariate and bivariate parameters, respectively. Results: Results showed that the phenol content of AETD (54.13 ± 0.14 mg GAE/g extract) was higher than that of flavonoids (16.73 ± 0.06 mg GAE/g extract), tannins (12.08 ± 0.07 mg GAE/g extract), and saponins (IC50 = 13.56 ± 0.03 mg DE/g extract). AETD showed a higher inhibitory potential on α-glucosidase activity (IC50 = 191.51 ± 5.63 μg/mL) than on α-amylase activity (IC50 = 1774.90 ± 10.32 μg/mL). AETD (250 and/or 500 mg/kg) prevented drastic loss of body weight and reduced food and water consumption in insulin resistant rats. The levels of blood glucose, total cholesterol, triglycerides, low-density lipoprotein cholesterol, and malondialdehyde were also reduced while high-density lipoprotein cholesterol level, reduced glutathion level, and catalase and superoxide dismutase activity increased after administration of AETD (250 and 500 mg/kg) in insulin resistant rats. Conclusion: AETD has significant antihyperglycemic, antidyslipidemic, and antioxidant potential, thus it can be used for the management of type 2 diabetes mellitus and its complications

Effect of Aqueous Extract of Adansonia digitata Stem Bark on the Development of Hypertension in L-NAME-Induced Hypertensive Rat Model

Background. Adansonia digitata is a plant used against cardiovascular disorders in African folk medicine. We assessed the effects of the aqueous extract of its stem bark on the development of hypertension in L-NAME-induced hypertensive rats. Methods. The animals were administered L-NAME once daily for 3 weeks (25 mg/kg, i.p.), concomitantly with aqueous extract of A. digitata stem bark (100 and 200 mg/kg, p.o.) or captopril (20 mg/kg, p.o.). Then, hemodynamic and electrocardiographic parameters, oxidative stress markers, and the lipid profile were assessed in the blood and heart, aorta, and kidney homogenates, and histopathological analyses were performed. Results. L-NAME-induced hypertensive control animals, but not the animals concomitantly treated with A. digitata extract, displayed increases in the mean arterial blood pressure (21.64% difference, p<0.001, vs. dose 200 mg/kg), systolic arterial blood pressure (21.33%, p<0.001), and the diastolic arterial blood pressure (21.84%, p<0.001). In addition, hypertensive control animals displayed (i) increases in serum triglycerides, total cholesterol, LDL, and creatinine levels, malondialdehyde and transaminase activities, and atherogenic index; (ii) decreases in serum HDL, catalase, reduced glutathione, and nitric oxide; and (iii) aorta wall thickening, inflammatory cell infiltration, and cell loss in the cardiac muscle and renal tissues. As captopril, the extract prevented hypertension-like changes in lipid profile, cardiac, hepatic, and renal affection indicators, and oxidative stress markers. Conclusion. Our findings suggest that the extract of A. digitata has antihypertensive and antioxidant effects in L-NAME-induced hypertension rat models. These effects partly justify the traditional medicine use against cardiovascular disorders

Potential Curative Effects of Aqueous Extracts of Cissus quadrangularis (Vitaceae) and Jatropha gossypiifolia (Euphorbiaceae) on Acetaminophen-Induced Liver Injury in Mice

ABSTRACT: Background: Acetaminophen-induced liver injury remains a significant public health problem because available treatments are limited due to their adverse effects. Medicinal plants, which are an important source of bioactive molecules, could be an alternative treatment for liver disease. Objective: This study was designed to investigate the curative effect of aqueous extracts of Cissus quadrangularis (Vitaceae) and Jatropha gossypiifolia (Euphorbiaceae) on acetaminophen-induced liver injury in mice. Methods: Mice were divided into groups and treated with distilled water, silymarin (50 mg/kg), a reference hepatoprotective agent, and aqueous extracts of C quadrangularis and J gossypiifolia (50 and 100 mg/kg, PO, respectively). These substances were given as a single daily dose 4 hours after acetaminophen administration (300 mg/kg, PO) for 2 days. Mice were humanely put to death 24 hours after the last dose and serum alanine aminotransferase and aspartate aminotransferase activities, total bilirubin and protein levels, reduced glutathione, superoxide dismutase, malondialdehyde, catalase, and nitrite tissue levels were assessed. Histology of the livers of the mice was performed by hematoxylin and eosin staining. Results: Acetaminophen administration induced a significant (P < 0.05) mean (SEM) body weight loss (–14.45% [5.92%]), a significant elevation of alanine aminotransferase activity (15.08%), total protein and bilirubin levels (25.80%), and a significant (P < 0.05) increase in liver superoxide dismutase (67.71%), catalase (63.00%), glutathione (40.29%), malondialdehyde (30.67%), and nitrite levels compared with the control group. In curative treatment, C quadrangularis and J gossypiifolia (50 and 100 mg/kg) significantly (P < 0.05) reduced mean (SEM) body weight loss (16.67% [7.16%] and 1.25% [0.51%], respectively), serum alanine aminotransferase activity (17.62% and 11.14%, respectively), bilirubin level (29.62% and 49.14%, respectively) compared with acetaminophen group, and J gossypiifolia normalized serum total protein level. Both extracts significantly (P < 0.05) reduced the levels of glutathione and malondialdehyde and normalized that of nitrite, superoxide dismutase, and catalase compared with the acetaminophen group. Hepatocyte necrosis and inflammatory cell infiltration were remarkably reduced by the plant extracts. Conclusions: The results obtained are evidence in favor of the development of a formulation based on the extracts of these plants against liver diseases