18 research outputs found
Anaerobic biodigestion of pigs feces in the initial, growing and finishing stages fed with diets formulated with corn or sorghum
Potential of antimicrobial volatile organic compounds to control Sclerotinia sclerotiorum in bean seeds
Predição de curvas de crescimento de tecidos de fêmeas suínas por intermédio da função alométrica estendida
The influence of environmental variables on the functional structure of headwater stream fish assemblages: a study of two tropical basins in Central Brazil
Lisina digestível para suínos machos castrados de alta deposição de carne submetidos a estresse por calor dos 30 aos 60 kg
Ractopamine and lysine levels on performance and carcass characteristics of finishing pigs
An Initial Evaluation of Gellan Gum as a Material for Tissue Engineering Applications
Alpha-modified minimum essential medium (alphaMEM) has been found to cross-link a 1% gellan gum solution, resulting in the formation of a self-supporting hydrogel in 1:1 and 5:1 ratios of polysaccharide: alphaMEM. Rheological data from temperature sweeps confirm that in addition to orders of magnitude differences in G' between 1% gellan and 1% gellan with alphaMEM, there is also a 20°C increase in the temperature at which the onset of gelation takes place when alphaMEM is present. Frequency sweeps confirm the formation of a true gel; mechanical spectra for mixtures of gellan and alphaMEM clearly demonstrate G' to be independent of frequency. It is possible to immobilize cells within a three-dimensional (3D) gellan matrix that remain viable for up to 21 days in culture by adding a suspension of rat bone marrow cells (rBMC) in alphaMEM to 1% gellan solution. This extremely simple approach to cell immobilization within 3D constructs, made possible by the fact that gellan solutions cross-link in the presence of millimolar concentrations of cations, poses a very low risk to a cell population immobilized within a gellan matrix and thus indicates the potential of gellan for use as a tissue engineering scaffold