Developments in Rapid Detection Methods for the Detection of Foodborne Campylobacter in the United States

The accurate and rapid detection of Campylobacter spp. is critical for optimal surveillance throughout poultry processing in the United States. The further development of highly specific and sensitive assays to detect Campylobacter in poultry matrices has tremendous utility and potential for aiding the reduction of foodborne illness. The introduction and development of molecular methods such as polymerase chain reaction (PCR) have enhanced the diagnostic capabilities of the food industry to identify the presence of foodborne pathogens throughout poultry production. Further innovations in various methodologies, such as immune-based typing and detection as well as high throughput analyses, will provide important epidemiological data such as the identification of unique or region-specific Campylobacter. Comparable to traditional microbiology and enrichment techniques, molecular techniques/methods have the potential to have improved sensitivity and specificity, as well as speed of data acquisition. This review will focus on the development and application of rapid molecular methods for identifying and quantifying Campylobacter in U.S. poultry and the emergence of novel methods that are faster and more precise than traditional microbiological techniques

PVC-LOT-004-D-137

This article discusses the role of embodiment in judgment and choice to (a) attain clarity on conceptual and methodological issues by presenting a literature review of prior empirical research on embodiment, (b) gain an integrative view on the topic of embodiment in judgment and choice by proposing somatic marker theory as a unifying conceptual framework for bridging cognition and affect in terms of embodiment, and (c) discuss and clarify ideas and directions for further research on the topic

Phase based x ray scattering A preliminary study to detect cancer cells in a very early stage

Purpose This theoretical work contains a detailed investigation of the potential and sensitivity of phase based x ray scattering for cancer detection in biopsies if cancer is in a very early stage of development. Methods Cancer cells in their early stage of development differ from healthy ones mainly due to their faster growing cell nuclei and the enlargement of their densities. This growth is accompanied by an altered nucleus plasma relation for the benefit of the cell nuclei, that changes the physical properties especially the index of refraction of the cell and the one of the cell nuclei. Interaction of radiation with matter is known to be highly sensitive to small changes of the index of refraction of matter; therefore a detection of such changes of volume and density of cell nuclei by means of high angular resolved phase based scattering of x rays might provide a technique to distinguish malignant cells from healthy ones if the cell cell nucleus system is considered as a coherent phase shifting object. Then one can observe from a thin biopsy which represents a monolayer of cells no multiple scattering that phase based x ray scattering curves from healthy cells differ from those of cancer cells in their early stage of development. Results Detailed calculations of x ray scattering patterns from healthy and cancer cell nuclei yield graphs and numbers with which one can distinguish healthy cells from cancer ones, taking into account that both kinds of cells occur in a tissue within a range of size and density. One important result is the role and the influence of the lateral coherence width of the radiation on the scattering curves and the sensitivity of phase based scattering for cancer detection. A major result is that a larger coherence width yields a larger sensitivity for cancer detection. Further import results are calculated limits for critical sizes and densities of cell nuclei in order to attribute the investigated tissue to be healthy or diseased. Conclusions With this proposed method it should be in principle possible to detect cancer cells in apparently healthy tissues in biopsies and or in samples of the far border region of abscised or excised tissues. Thus this method could support established methods in diagnostics of cancer suspicious samples. 2014 American Association of Physicists in Medicine. [http dx.doi.org 10.1118 1.4871616

Calculation of scattering patterns from phase shifting objects using the Radon Transform

In the case of neutron (and X-ray) scattering by objects that are about 105times larger than the wavelength, the objects can be considered as (inhomogeneous) phase-shifting media. In contrast with small-angle scattering, the scattering patterns from phase-shifting objects are calculated by the superposition of coherent partial waves that penetrate the object. In order to determine the scattering patterns from large complicated objects, it is proposed to use the two-dimensional Radon transform of the objects and Fraunhofer diffraction. This approach is much easier than using the small-angle scattering treatment, as is shown in this paper.</jats:p

Corrigendum Calculation of scattering patterns from phase shifting objects using the Radon transform

The affiliations for the authors of J. Appl. Cryst. (2011), 44, 1157–1163 are corrected.</jats:p