The Role of Adipocytokines in Colon Cancer and Adenomas

Background: Metabolic changes resulting from obesity, insulin insensitivity, and imbalances in hormones such as adiponectin, leptin, resistin, apelin and visfatin, which are derived from white adipose tissue-derived hormone, are directly linked to both colon cancer (CC) and inflammatory bowel diseases increasing tissue-derived risk. We conducted this study to evaluate the relationship between the circulating concentrations of adiponectin, leptin, resistin, apelin and visfatin and colon adenoma and CC. Methods: Our study included 90 participants aged >18 years who were divided into three groups: colon cancer, adenoma and control. The serum concentrations of the investigated adipohormones were measured with ELISA in 30 patients with colon adenoma, 30 with CC and 30 controls with no colon pathology. Results: Demographic, anthropometric, metabolic and hormonal parameters were also recorded. The group means were compared by using one-way analysis of variance (ANOVA). Dual comparisons between groups were analyzed with the Tukey test. Pearson correlation coefficient was used to determine the relation between continuous variables. Adiponectin and leptin levels in patients with adenomas (p<0.000; p<0.000, respectively) and CC (p<0.000; p<0.000, respectively) were lower than in controls. Apelin level in patients with CC (p<0.000; p<0.000, respectively) was lower than in patients with adenomas and in controls. Resistin and visfatin levels in patients with CC (p<0.000; p<0.000, respectively) were higher than in patients with adenomas and in controls. Conclusions: We have concluded that adiponectin, leptin, resistin, apelin and visfatin levels may play an important role in colon carcinogenesis. We also assume that adiponectin and leptin may be associated with the risk of colon adenoma

Iron Deficiency Anaemia is Associated with Decreased Levels of Macrophage Migration Inhibitory Factor and Monocyte Chemoattractant Protein-1

Many bodily systems are affected by iron deficiency anaemia (IDA), including the immune system. However, the pathophysiological mechanisms whereby this anaemia promotes deterioration in immunity remain largely unexplained. In order to enlighten this pathophysiological link, serum levels of macrophage migration inhibitory factor (MIF) and monocyte chemoattractant protein-1 (MCP-1), which play an important roles in a healthy functioning immune system, were examined in patients with IDA and healthy volunteers. A total of 30 patients with IDA (mean age 33.6+/-7.8 years, 30% male) and 30 healthy individuals (mean age 30.1 +/- 8.8 years, 27 % male) were included. Serum MIF and MCP-1 levels were measured in a sandwich-assay format, using commercial enzyme-linked immunosorbent assay kits. There was no statistically significant difference in age and gender distribution between participants with IDA and controls (p> 0.05). Serum MIF and MCP-1 concentrations were lower in the IDA group than in the control group (p < 0.005, and p < 0.01, respectively). When considering the role of MIF and MCP-1 in maintaining the normal immune response of the organism, a decrease in production in patients with IDA may contribute to immune dysfunction in these individuals

Investigation of serum macrophage migration inhibitor factor and monocyte chemotactic protein-1 levels in irritable bowel syndrome

Aim: Irritable bowel syndrome (IBS), a functional disorder of the bowel, has been thought to result from immune activation. The aim of this study was to evaluate macrophage migration inhibitory factor (MMIF) and monocyte chemotactic protein-1 (MCP-1) levels in IBS patients. Materials and methods: We enrolled 30 IBS patients and 30 healthy controls. The MMIF and MCP-1 levels of all patients and controls were detected using commercial enzyme-linked immunosorbent assay kits. Results: Serum MMIF and MCP-1 levels were markedly higher in IBS patients than in controls. White blood cell, neutrophil, lymphocyte, monocyte, eosinophil, and basophil counts did not differ significantly between groups. Conclusion: These results show that alterations in MMIF and MCP-1 affect the proinflammatory process. They also suggest that MMIF and MCP-1 may play a substantial role in IBS

The Relationship Between Recurrent Aphtous Stomatitis and Macrophage Migration Inhibitory Factor Levels

AimRecurrent aphtous stomatitis (RAS) is the most common mucosal disease with unknown etiology. Macrophage migration inhibitory factor (MIF) is a proinflamatory cytokine which has a role in T cell activation. This study aimed to investigate importance of MIF in etiology of RAS.Materials and Methods70 patients who were above the age of 18, clinically diagnosed RAS and 72 healthy volunteers were included into the study. Patients and control subjects were examined for MIF seroprevalence by ELISA.ResultsThere were no significant difference between RAS and control groups in mean values of MIF. In comparison of RAS patients in two subgroups- with RAS score above 6 and with 5 and less than 5, there were also no significant difference between these subgroups in mean values of MIF. On the other hand the reccurrence of lesions and mean values of MIF showed no relevance.ConclusionEventhough proposed in the hypothesis of this study, there had been no statistically significant relationship between MIF and RAS etiology

Rekürrent aftöz stomatit hastalarında “makrofaj migrasyon inhibitör faktör” değerlerinin normal populasyon ile kıyaslanması

Rekürren aftöz stomatit(RAS) en sık rastlanılan ağız mukozası hastalıklarından biridir. Olası etyolojik faktörleri lokal ve sistemik koşullar ile genetik, immünolojik ve mikrobiyal faktörlerdir. Pek çok çalışmada hücresel immünitenin özellikle T hücre alt tiplerinin etyolojide yeri gösterilmiştir. Makrofaj migrasyon inhibitör faktör(MIF) ilk kez makrofajları inflamasyon alanına yoğunlaştıran bir lenfokin olarak tanımlanmış ve hücresel immünitede önemli rolü olduğu gösterilmiştir.Bu çalışmamızın amacı RAS hastalarında serum MIF konsantrasyonlarının normal populasyon arasındaki farkı kıyaslayarak MIF’in RAS oluşumundaki yerini araştırmaktır.Özet:Rekürren aftöz stomatit(RAS) en sık rastlanılan ağız mukozası hastalıklarından biridir. Olası etyolojik faktörleri lokal ve sistemik koşullar ile genetik, immünolojik ve mikrobiyal faktörlerdir. Pek çok çalışmada hücresel immünitenin özellikle T hücre alt tiplerinin etyolojide yeri gösterilmiştir. Makrofaj migrasyon inhibitör faktör(MIF) ilk kez makrofajları inflamasyon alanına yoğunlaştıran bir lenfokin olarak tanımlanmış ve hücresel immünitede önemli rolü olduğu gösterilmiştir.Bu çalışmamızın amacı RAS hastalarında serum MIF konsantrasyonlarının normal populasyon arasındaki farkı kıyaslayarak MIF’in RAS oluşumundaki yerini araştırmaktır.Özet:Rekürren aftöz stomatit(RAS) en sık rastlanılan ağız mukozası hastalıklarından biridir. Olası etyolojik faktörleri lokal ve sistemik koşullar ile genetik, immünolojik ve mikrobiyal faktörlerdir. Pek çok çalışmada hücresel immünitenin özellikle T hücre alt tiplerinin etyolojide yeri gösterilmiştir. Makrofaj migrasyon inhibitör faktör(MIF) ilk kez makrofajları inflamasyon alanına yoğunlaştıran bir lenfokin olarak tanımlanmış ve hücresel immünitede önemli rolü olduğu gösterilmiştir.Bu çalışmamızın amacı RAS hastalarında serum MIF konsantrasyonlarının normal populasyon arasındaki farkı kıyaslayarak MIF’in RAS oluşumundaki yerini araştırmaktır.Özet:Rekürren aftöz stomatit(RAS) en sık rastlanılan ağız mukozası hastalıklarından biridir. Olası etyolojik faktörleri lokal ve sistemik koşullar ile genetik, immünolojik ve mikrobiyal faktörlerdir. Pek çok çalışmada hücresel immünitenin özellikle T hücre alt tiplerinin etyolojide yeri gösterilmiştir. Makrofaj migrasyon inhibitör faktör(MIF) ilk kez makrofajları inflamasyon alanına yoğunlaştıran bir lenfokin olarak tanımlanmış ve hücresel immünitede önemli rolü olduğu gösterilmiştir.Bu çalışmamızın amacı RAS hastalarında serum MIF konsantrasyonlarının normal populasyon arasındaki farkı kıyaslayarak MIF’in RAS oluşumundaki yerini araştırmaktır.Introduction Recurrent aphtous stomatitis (RAS) is the most common mucosal disease with unknown etiology. Macrophage inhibition factor(MIF) is a proinflamatory cytokine which has a role in T cell activation. This study aimed to investigate importance of MIF in etiology of RAS. Material and Methods 70 patients who were above the age of 18, clinically diagnosed RAS and 72 healthy volunteers were included into the study. Patients and control subjects were examined for MIF seroprevalence by ELISA. Results There were no significant difference between RAS and control groups in mean values of MIF. In comparison of RAS patients in two subgroups- with RAS score above 6 and with 5 and less than 5, there were also no significant difference between these subgroups in mean values of MIF. On the other hand; the reccurrence of lesions and mean values of MIF showed no relevance. Conclusion Eventhough proposed in the hypothesis of this study, there had been no statistically significant relationship between MIF and RAS etiology

Yağ Kütle ve Kantitatif İnsulin Sensitivite Kontrol İndekslerinden Türetilmiş Yeni Bir İnsulin Sensitivite İndeksi

Aim Obesity has recently become one of the most important health problems throughout the world. This fact led to the controversies on the clinical use of insulin sensitivity indices. Indices previously described or introduced in this study have been evaluated to choose one, which is capable of exhibiting significant distinctions between healthy children and those involved in the classes of childhood obesity. Material and Methods A total of 179 girls; 81 morbidly obese(MO), 42 obese(O), 16 overweight(OW) and 40 normal(N) participated in the study. Groups were constituted based upon age- and sex-specific body mass index percentiles tabulated by World Health Organization. Homeostasis Model Assessment of Insulin Resistance(HOMA-IR), HOMA-IR/BMI, log HOMA-IR, fasting glucose/fasting insulin ratio(FGIR), quantitative insulin sensitivity check index(QUICKI), Raynaud, reciprocal insulin indices and also new indices HOMAIR*BMI, HOMA-IR*fat mass index(FMI), QUICKI*BMI, QUICKI*FMI were calculated. The cut-offs 3.16 and 2.5 for HOMA-IR, 7 and 6 for FGIR, 0.357 and 0.328 for QUICKI were evaluated to estimate insulin resistance. Statistical analyses were performed with Predictive Analytics SoftWare(PASW) Statistics 18. Results and Conclusion QUICKI*FMI was able to make a clear-cut separation between the groups. A new trilogy for cut-offs (HOMA>2.5, FGIR2.5, FGIR<7, QUICKI<0.328) ortaya kondu. QUICKI nin çok yönlü özelliği gözlendi. QUICKI nin, 0.328 cut-off noktası kullanıldığında MO i O den, 0.357 cut-off noktası kullanıldığında ise O i OW den ayırt edebildiği belirlendi. QUICKI*FMI indeksinin, çocukluk çağı obezitesi bağlamında N-OW, OW-O ve O-MO gruplar arasındaki farklılıkların ileri düzeyde (p?0.005) tanımlanmasında eşsiz bir indeks olduğu sonucuna varıldı

(The prevalence of gestational diabetes mellitus in NamÕk Kemal University Training and Research Hospital)

Giriş: Her yıl önemli sayıda kadını etkileyen Gestasyonel diabetes mellitus (GDM) hem gebeler hem de bebekleri için istenmeyen sağlık problemlerine yol açmaktadır. Bu çalışma, Namık Kemal Üniversitesi Araştırma ve Uygulama Hastanesinde Gestasyonel Diyabetes Mellitus prevalansını değerlendirmeyi ve diğer çalışmaların sonuç- larıyla karşılaştırmayı amaçlamaktadır. Materyal ve Metot: Ocak 2010 ile Mayıs 2013 tarihleri arasında 24 28 Haftalık gebelik süresinde Namık Kemal Üniversitesi Araştırma ve Uygulama Hastanesinde kabul edilen gebeler çalışmaya dahil edildi. Yapılan dosya taramasında 502 gebeye 50 gram Glukoz Yükleme Testi (GYT) uygulandığı, test sonuçlarına göre bunların 29una da 100 gram Oral Glukoz Tolerans Testi (OGTT) yapıldığı tespit edildi. Bulgular: Gebelerin tamamına yapılan 50 g Glukoz Yükleme Testi 29 olguda pozitif olarak bulundu. Tarama testi yapılan gebelerin 13 tanesinde 100 g OGTT pozitif olarak değerlendirilmiş ve hastanemize başvuran gebelerin gestasyonel diyabet prevelansı Carpenter ve Caustan kriterlerine göre %2,59 bulunmuştur. Sonuçlar: Gestasyonel diabetes mellitus prevalansı Namık Kemal Üniversitesi Araştırma ve Uygulama Hastanesinde Türkiyenin diğer bölgelerinden rapor edilen epidemiyolojik çalışmalara göre daha düşük bulundu.Background: Gestational diabetes mellitus (GDM) affects a significant number of women each year and it is associated with a wide range of adverse outcomes for pregnants and their babies. The present study aimed to evaluate the prevalence of Gestational diabetes mellitus in Nam&Otilde;k Kemal University Training and Research Hospital and to compare the results of other studies. Material and Methods: From January 2010 to May 2013, patients admitted to gynecology clinic with 24 28 week pregnancy were studied. A glucose challenge test (GCT) was given to 502 pregnant women and based on the results 29 went on to take an oral glucose tolerance test (OGTT). Results: 502 pregnant women tested, Fifty gram Glucose Challenge Test was performed on all pregnant women which was positive only in 29 cases, and in 13 of them 100 gram oral glucose tolerance test was positive The prevalence of gestational diabetes in pregnant women who were admitted to our hospital was 2.59% according to Carpenter and Caustan diagnostic criteria Conclusions: The prevalence of Gestational diabetes mellitus was found to be lower in in Nam&Otilde;k Kemal University Training and Research Hospital rather than the other regions of Turkey as reported in epidemiological studies

Adenomatöz kolon polipli hastalarda oksidatif stres mekanizmasının paraoksonaz ve arilesteraz üzerinden değerlendirilmesi

Amaç: Adenomatöz polip, rektum ve kolonda adenomların lümene doğru gelişimi ile karakterize klinik bir durumdur. Birçok dejeneratif ve tümöral hastalığın patogenezinde artmış oksidatif stres rol oynamaktadır. Bu çalışma adenomatöz kolon polipli hastalarda paraoksonaz, indüklenebilir paraoksonaz ve arilesteraz enzim aktivitelerinin tespiti ve oksidatif stres ile hastalığın patofizyolojisi arasındaki ilişkiyi ortaya koymak amacı ile planlanmıştır. Gereç ve yöntem: Namık Kemal Üniversitesi Araştırma ve Uygulama Hastanesi Gastroenteroloji Polikliniği’ne başvuran hastalardan kolon polipi saptananlar ve sağlıklı gönüllüler çalışmaya alındı. Paraoksonaz, indüklenebilir paraoksonaz ve arilesteraz düzeylerinin ölçümleri spektrofotometrik olarak yapıldı. Bulgular: Kolon polipli hastalar ile sağlıklı kontroller karşılaştırıldığında, kolon polipli hastalarda, paraoksonaz, indüklenebilir paraoksonaz aktiviteleri istatistiksel olarak anlamlı oranda düşük bulunurken, arilesteraz aktivitesinde istatistiksel olarak anlamlı fark bulunmadı. Sonuç: Kolon polipli hastalarda paraoksonaz ve indüklenebilir paraoksonaz aktivitesinin sağlıklı polülasyondan düşük bulunması, oksidan-antioksidan dengenin oksidan yönünde bozulmasının polipli hastalarda polip gelişimi ile yakın ilişki içinde olduğunu düşündürmektedi

A New Insulin Sensitivity Index Derived From Fat Mass Index and Quantitative Insulin Sensitivity Check Index

AimObesity has recently become one of the most important health problems throughout the world. This fact led to the controversies on the clinical use of insulin sensitivity indices. Indices previously described or introduced in this study have been evaluated to choose one, which is capable of exhibiting significant distinctions between healthy children and those involved in the classes of childhood obesity.Material and MethodsA total of 179 girls; 81 morbidly obese(MO), 42 obese(O), 16 overweight(OW) and 40 normal(N) participated in the study. Groups were constituted based upon age- and sex-specific body mass index percentiles tabulated by World Health Organization. Homeostasis Model Assessment of Insulin Resistance(HOMA-IR), HOMA-IR/BMI, log HOMA-IR, fasting glucose/fasting insulin ratio(FGIR), quantitative insulin sensitivity check index(QUICKI), Raynaud, reciprocal insulin indices and also new indices HOMA-IR* BMI, HOMA-IR*fat mass index(FMI), QUICKI*BMI, QUICKI*FMI were calculated. The cut-offs 3.16 and 2.5 for HOMA-IR, 7 and 6 for FGIR, 0.357 and 0.328 for QUICKI were evaluated to estimate insulin resistance. Statistical analyses were performed with Predictive Analytics SoftWare(PASW) Statistics 18.Results and ConclusionQUICKI*FMI was able to make a clear-cut separation between the groups. A new trilogy for cut-offs (HOMA>2.5, FGIR<7, QUICKI<0.328); each giving the similar results, has been suggested. Multifaceted character of QUICKI was also introduced. QUICKI was capable of discriminating MO from O when 0.328 cutoff was used, and O from OW when 0.357 cut-off was used. QUICKI*FMI index, a new one, was unique in detecting the advanced level of differences(p≤0.005) between N-OW, OW-O and O-MO groups during childhood obesity

The Relationship Neutrophil-Lymphocyte Ratio and Heart Rate Recovery

AimIt has been shown that heart rate recovery index is an independent risk factor for cardiovascular mortality. It has been reported in most epidomiologic studies that an elevated neutrophil-lymphocyte ratio is strongly associated with cardiovascular diseases. The aim of this study was to investigate whether there is a relationship between heart rate recovery index and neutrophillymphocyte ratio.Materials and MethodsA total of 238 patients who underwent coronary angiography due to abnormal treadmill exercise test results and who admitted to Cardiology Department of Faculty of Medicine at Namık Kemal University were evaluated retrospectively. heart rate recovery index was accepted as abnormal when the difference between peak heart rate at maximal exercise and the heart rate at the first minute of recovery phase is 18 beat or less.ResultsIn the comparison between those with abnormal heart rate recovery index and those with normal heart rate recovery index in terms of neutrophil count and neutrophil-lymphocyte ratio was found a significant relationship. (respectively, p=0,027 ve p=0.000).ConclusionConsequently, our findings may potentially suggest that an elevated neutrophil-lymphocyte ratio in patients with abnormal heart rate recovery index might be related to cardiovascular mortality and morbidity. The investigation of the prognostic significance of elevated neutrophil-lymphocyte ratio in patients with abnormal heart rate recovery index in future prospective studies will be provide more conclusive evidence