Nontumoral portal vein thrombosis in patients with and without cirrhosis : clinical significance, natural history of varices and efficacy of anticoagulation

A cirrose caracteriza-se por um estado de hipercoagulabilidade. A incidência de trombose da veia porta (TVP) aumenta na cirrose avançada. O impacto da TVP na história natural de cirrose não é claro. A segurança de anticoagulação na cirrose avançada e TVP não é clara e o seu impacto na sobrevida livre de transplante hepático ortotopico (THO) na cirrose avançada e TVP é controverso. Em doentes com trombose da veia porta não-cirrótica e não-tumoral (TVPNCNT) crónica, existe escassa evidência sobre a história natural de varizes esófagogastricas que são habitualmente manejadas como na cirrose. Não existe evidência relativa à eficácia desta estratégia. No primeiro estudo desta tese, uma coorte de 241 doentes com cirrose sem TVP foi seguida prospectivamente e verificamos que a descompensação prévia de cirrose e trombocitopenia foram factores independentes predizentes de desenvolvimento de TVP. A TVP não foi factor predizente independente de descompensações de cirrose ou de sobrevida livre de THO. No segundo estudo, uma análise retrospectiva duma coorte prospectiva de 178 doentes com TVPNCNT crónica, verificamos que o curso natural de varizes esofagogástricas é semelhante ao de doentes com cirrose. No terceiro estudo, uma análise retrospectiva de 80 doentes com cirrose e TVP não-tumoral, constatamos que a anticoagulação, embora não melhorasse a sobrevida global livre de THO, associou-se a melhoria de sobrevida apenas em doentes com cirrose avançada. A anticoagulação aumentou significativamente a recanalização da TVP e deverá ser mantida para prevenir a retrombose. Em conclusão, a descompensação prévia de cirrose e trombocitopenia predizem independentemente, maior risco de desenvolvimento de TVP. A TVP não influenciou independentemente, descompensações de cirrose ou a sobrevida livre de THO. A anticoagulação foi segura e aparenta melhorar a sobrevida livre de THO na cirrose avançada. Na TVPNCNT crónica a mesma estratégia terapêutica como na cirrose associou-se a um baixo risco de hemorragia e mortalidade.Cirrhosis is considered a hipercoagulable state and there is strong evidence that the incidence of nontumoral portal vein thrombosis (PVT) increases in advanced cirrhosis. There is conflicting data regarding the impact of PVT on the natural history of cirrhosis. The safety of anticoagulation especially in patients with advanced cirrhosis and PVT is unclear. The impact of anticoagulation in patients with cirrhosis and PVT on orthotopic liver transplant (OLT) free survival is controversial. There is scant data regarding natural history of gastroesophageal varices in patients with chronic noncirrhotic nontumoral PVT (NCNTPVT), which are usually managed as in cirrhosis. There is no evidence regarding efficacy of this policy. In the first study of this thesis, a cohort of 241 patients with cirrhosis without PVT at study inclusion were evaluated prospectively and we found that prior decompensation of cirrhosis and thrombocytopenia independently predicted development of PVT. PVT did not independently predict higher risk of cirrhosis decompensations and lower OLT free survival. In the second study, a retrospective analysis of a prospective cohort of 178 patients with chronic NCNTPVT, we found that the course of esophagogastric varices appears to be similar to that in cirrhosis. In the third study, a retrospective analysis of 80 patients with cirrhosis with nontumoral PVT, although anticoagulation did not improve OLT free survival of the entire study cohort, it was associated with significantly higher survival in advanced cirrhosis. Anticoagulation was safe, significantly increased PVT recanalization and should be maintained to avoid rethrombosis. In conclusion, prior decompensation of cirrhosis and thrombocytopenia predicted higher risk of developing PVT. PVT did not independently influence cirrhosis decompensations or OLT free survival. Anticoagulation appears to improve OLT free survival only in advanced cirrhosis. In chronic NCNTPVT using the same therapeutic approach as for cirrhosis was associated with a low risk of bleeding and death

SUCESSO NO TRATAMENTO ENDOSCÓPICO DE PERFURAÇÃO CÓLICA COM OVER-THE-SCOPE CLIP

Colonoscopic perforation is a rare adverse event and it’s incidence increases as more procedures are performed. Therapy may include closure with clip devices with acceptable results. This case-report demonstrates the efficacy and safety of the over-the-scope clip application in an iatrogenic colonic perforation. A perfuração durante colonoscopia é uma complicação rara, cuja incidência aumenta com a realização de procedimentos. O tratamento pode incluir o encerramento com recurso a clip, com resultado satisfatório. Este caso-clínico demonstra a eficácia e segurança da aplicação do over-the-scope clip no tratatamento de perfurações iatrogénicas do cólon.&nbsp

Characterization of novel calcium hydroxide- mediated highly porous chitosan- calcium scaffolds for potential application in dentin tissue engineering

The aim of this study was to develop a highly porous calcium- containing chitosan scaffold suitable for dentin regeneration. A calcium hydroxide (Ca[OH]2) suspension was used to modulate the degree of porosity and chemical composition of chitosan scaffolds. The chitosan solution concentration and freezing protocol were adjusted to optimize the porous architecture using the phase- separation technique. Scanning electron microscopy/energy- dispersive spectroscopy demonstrated the fabrication of a highly porous calcium- linked chitosan scaffold (CH- Ca), with a well- organized and interconnected porous network. Scaffolds were cross- linked on glutaraldehyde (GA) vapor. Following a 28- day incubation in water, cross- linked CH scaffold had no changes on humid mass, and CH- Ca featured a controlled degradability profile since the significant humid mass loss was observed only after 21 (26.0%) and 28- days (42.2%). Fourier- transform infrared spectroscopy indicated the establishment of Schiff base on cross- linked scaffolds, along with calcium complexation for CH- Ca. Cross- linked CH- Ca scaffold featured a sustained Ca2+ release up to 21- days in a humid environment. This porous and stable architecture allowed for human dental pulp cells (HDPCs) to spread throughout the scaffold, with cells exhibiting a widely stretched cytoplasm; whereas, the cells seeded onto CH scaffold were organized in clusters. HDPCs seeded onto CH- Ca featured significantly higher ALP activity, and gene expressions for ALP, Col1, DMP- 1, and DSPP in comparison to CH, leading to a significant 3.5 times increase in calcium- rich matrix deposition. In sum, our findings suggest that CH- Ca scaffolds are attractive candidates for creating a highly porous and bioactive substrate for dentin tissue engineering.Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/155906/1/jbmb34586.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/155906/2/jbmb34586_am.pd

Geography and ecology shape the phylogenetic composition of Amazonian tree communities

Aim: Amazonia hosts more tree species from numerous evolutionary lineages, both young and ancient, than any other biogeographic region. Previous studies have shown that tree lineages colonized multiple edaphic environments and dispersed widely across Amazonia, leading to a hypothesis, which we test, that lineages should not be strongly associated with either geographic regions or edaphic forest types. Location: Amazonia. Taxon: Angiosperms (Magnoliids; Monocots; Eudicots). Methods: Data for the abundance of 5082 tree species in 1989 plots were combined with a mega-phylogeny. We applied evolutionary ordination to assess how phylogenetic composition varies across Amazonia. We used variation partitioning and Moran\u27s eigenvector maps (MEM) to test and quantify the separate and joint contributions of spatial and environmental variables to explain the phylogenetic composition of plots. We tested the indicator value of lineages for geographic regions and edaphic forest types and mapped associations onto the phylogeny. Results: In the terra firme and várzea forest types, the phylogenetic composition varies by geographic region, but the igapó and white-sand forest types retain a unique evolutionary signature regardless of region. Overall, we find that soil chemistry, climate and topography explain 24% of the variation in phylogenetic composition, with 79% of that variation being spatially structured (R$^{2}$ = 19% overall for combined spatial/environmental effects). The phylogenetic composition also shows substantial spatial patterns not related to the environmental variables we quantified (R$^{2}$ = 28%). A greater number of lineages were significant indicators of geographic regions than forest types. Main Conclusion: Numerous tree lineages, including some ancient ones (>66 Ma), show strong associations with geographic regions and edaphic forest types of Amazonia. This shows that specialization in specific edaphic environments has played a long-standing role in the evolutionary assembly of Amazonian forests. Furthermore, many lineages, even those that have dispersed across Amazonia, dominate within a specific region, likely because of phylogenetically conserved niches for environmental conditions that are prevalent within regions

Geography and ecology shape the phylogenetic composition of Amazonian tree communities

AimAmazonia hosts more tree species from numerous evolutionary lineages, both young and ancient, than any other biogeographic region. Previous studies have shown that tree lineages colonized multiple edaphic environments and dispersed widely across Amazonia, leading to a hypothesis, which we test, that lineages should not be strongly associated with either geographic regions or edaphic forest types.LocationAmazonia.TaxonAngiosperms (Magnoliids; Monocots; Eudicots).MethodsData for the abundance of 5082 tree species in 1989 plots were combined with a mega-phylogeny. We applied evolutionary ordination to assess how phylogenetic composition varies across Amazonia. We used variation partitioning and Moran's eigenvector maps (MEM) to test and quantify the separate and joint contributions of spatial and environmental variables to explain the phylogenetic composition of plots. We tested the indicator value of lineages for geographic regions and edaphic forest types and mapped associations onto the phylogeny.ResultsIn the terra firme and várzea forest types, the phylogenetic composition varies by geographic region, but the igapó and white-sand forest types retain a unique evolutionary signature regardless of region. Overall, we find that soil chemistry, climate and topography explain 24% of the variation in phylogenetic composition, with 79% of that variation being spatially structured (R2 = 19% overall for combined spatial/environmental effects). The phylogenetic composition also shows substantial spatial patterns not related to the environmental variables we quantified (R2 = 28%). A greater number of lineages were significant indicators of geographic regions than forest types.Main ConclusionNumerous tree lineages, including some ancient ones (>66 Ma), show strong associations with geographic regions and edaphic forest types of Amazonia. This shows that specialization in specific edaphic environments has played a long-standing role in the evolutionary assembly of Amazonian forests. Furthermore, many lineages, even those that have dispersed across Amazonia, dominate within a specific region, likely because of phylogenetically conserved niches for environmental conditions that are prevalent within regions

Mapping density, diversity and species-richness of the Amazon tree flora

Using 2.046 botanically-inventoried tree plots across the largest tropical forest on Earth, we mapped tree species-diversity and tree species-richness at 0.1-degree resolution, and investigated drivers for diversity and richness. Using only location, stratified by forest type, as predictor, our spatial model, to the best of our knowledge, provides the most accurate map of tree diversity in Amazonia to date, explaining approximately 70% of the tree diversity and species-richness. Large soil-forest combinations determine a significant percentage of the variation in tree species-richness and tree alpha-diversity in Amazonian forest-plots. We suggest that the size and fragmentation of these systems drive their large-scale diversity patterns and hence local diversity. A model not using location but cumulative water deficit, tree density, and temperature seasonality explains 47% of the tree species-richness in the terra-firme forest in Amazonia. Over large areas across Amazonia, residuals of this relationship are small and poorly spatially structured, suggesting that much of the residual variation may be local. The Guyana Shield area has consistently negative residuals, showing that this area has lower tree species-richness than expected by our models. We provide extensive plot meta-data, including tree density, tree alpha-diversity and tree species-richness results and gridded maps at 0.1-degree resolution

Geographic patterns of tree dispersal modes in Amazonia and their ecological correlates

Aim: To investigate the geographic patterns and ecological correlates in the geographic distribution of the most common tree dispersal modes in Amazonia (endozoochory, synzoochory, anemochory and hydrochory). We examined if the proportional abundance of these dispersal modes could be explained by the availability of dispersal agents (disperser-availability hypothesis) and/or the availability of resources for constructing zoochorous fruits (resource-availability hypothesis). Time period: Tree-inventory plots established between 1934 and 2019. Major taxa studied: Trees with a diameter at breast height (DBH) ≥ 9.55 cm. Location: Amazonia, here defined as the lowland rain forests of the Amazon River basin and the Guiana Shield. Methods: We assigned dispersal modes to a total of 5433 species and morphospecies within 1877 tree-inventory plots across terra-firme, seasonally flooded, and permanently flooded forests. We investigated geographic patterns in the proportional abundance of dispersal modes. We performed an abundance-weighted mean pairwise distance (MPD) test and fit generalized linear models (GLMs) to explain the geographic distribution of dispersal modes. Results: Anemochory was significantly, positively associated with mean annual wind speed, and hydrochory was significantly higher in flooded forests. Dispersal modes did not consistently show significant associations with the availability of resources for constructing zoochorous fruits. A lower dissimilarity in dispersal modes, resulting from a higher dominance of endozoochory, occurred in terra-firme forests (excluding podzols) compared to flooded forests. Main conclusions: The disperser-availability hypothesis was well supported for abiotic dispersal modes (anemochory and hydrochory). The availability of resources for constructing zoochorous fruits seems an unlikely explanation for the distribution of dispersal modes in Amazonia. The association between frugivores and the proportional abundance of zoochory requires further research, as tree recruitment not only depends on dispersal vectors but also on conditions that favour or limit seedling recruitment across forest types

Pervasive gaps in Amazonian ecological research

Biodiversity loss is one of the main challenges of our time,1,2 and attempts to address it require a clear un derstanding of how ecological communities respond to environmental change across time and space.3,4 While the increasing availability of global databases on ecological communities has advanced our knowledge of biodiversity sensitivity to environmental changes,5–7 vast areas of the tropics remain understudied.8–11 In the American tropics, Amazonia stands out as the world’s most diverse rainforest and the primary source of Neotropical biodiversity,12 but it remains among the least known forests in America and is often underrepre sented in biodiversity databases.13–15 To worsen this situation, human-induced modifications16,17 may elim inate pieces of the Amazon’s biodiversity puzzle before we can use them to understand how ecological com munities are responding. To increase generalization and applicability of biodiversity knowledge,18,19 it is thus crucial to reduce biases in ecological research, particularly in regions projected to face the most pronounced environmental changes. We integrate ecological community metadata of 7,694 sampling sites for multiple or ganism groups in a machine learning model framework to map the research probability across the Brazilian Amazonia, while identifying the region’s vulnerability to environmental change. 15%–18% of the most ne glected areas in ecological research are expected to experience severe climate or land use changes by 2050. This means that unless we take immediate action, we will not be able to establish their current status, much less monitor how it is changing and what is being lostinfo:eu-repo/semantics/publishedVersio

Consistent patterns of common species across tropical tree communities

Trees structure the Earth’s most biodiverse ecosystem, tropical forests. The vast number of tree species presents a formidable challenge to understanding these forests, including their response to environmental change, as very little is known about most tropical tree species. A focus on the common species may circumvent this challenge. Here we investigate abundance patterns of common tree species using inventory data on 1,003,805 trees with trunk diameters of at least 10 cm across 1,568 locations1,2,3,4,5,6 in closed-canopy, structurally intact old-growth tropical forests in Africa, Amazonia and Southeast Asia. We estimate that 2.2%, 2.2% and 2.3% of species comprise 50% of the tropical trees in these regions, respectively. Extrapolating across all closed-canopy tropical forests, we estimate that just 1,053 species comprise half of Earth’s 800 billion tropical trees with trunk diameters of at least 10 cm. Despite differing biogeographic, climatic and anthropogenic histories7, we find notably consistent patterns of common species and species abundance distributions across the continents. This suggests that fundamental mechanisms of tree community assembly may apply to all tropical forests. Resampling analyses show that the most common species are likely to belong to a manageable list of known species, enabling targeted efforts to understand their ecology. Although they do not detract from the importance of rare species, our results open new opportunities to understand the world’s most diverse forests, including modelling their response to environmental change, by focusing on the common species that constitute the majority of their trees.Publisher PDFPeer reviewe