Ehrlich tumor induces TRPV1-dependent evoked and non-evoked pain-like behavior in mice

We standardized a model by injecting Ehrlich tumor cells into the paw to evaluate cancer pain mechanisms and pharmacological treatments. Opioid treatment, but not cyclooxygenase inhibitor or tricyclic antidepressant treatments reduces Ehrlich tumor pain. To best use this model for drug screening it is essential to understand its pathophysiological mechanisms. Herein, we investigated the contribution of the transient receptor potential cation channel subfamily V member 1 (TRPV1) in the Ehrlich tumor-induced pain model. Dorsal root ganglia (DRG) neurons from the Ehrlich tumor mice presented higher activity (calcium levels using fluo-4 fluorescent probe) and an increased response to capsaicin (TRPV1 agonist) than the saline-injected animals

Conhecimento materno sobre aleitamento: um estudo piloto realizado em Salvador, Bahia visando à elaboração de uma cartilha educativa

Although breast milk is the best food for the infants, maternal misinformation about the importance of breastfeeding is remarkable. We aimed to evaluate the knowledge of mothers and pregnant women about breastfeeding and to elaborate an educative booklet addressing the most important doubts identified in this population. A qualitative study was idealized and developed by Medicine graduating students of UFBA, attending at Preventive and Social Paediatrics' discipline. We studied a convenience sample, not probabilistic, composed by 24 mothers and pregnant women, using a semi-structured questionnaire elaborated by the authors to personal interview. The studied group presented doubts and misinformation about the subject, chiefly those related to breast preparing and feeding position (70.8%), besides the correct moment to introduce new foods (67.0%). The use of tea, water and juices before the sixth month is very frequent (50.0%), while 25.0% of the participants were afraid to have insufficient breast milk. Based on these data, an educative booklet was elaborated. We verified that the level of mothers' information about breastfeeding remains rather low in spite of the campaigns and the progress on scientific knowledge about the topic. The construction of an informative booklet should contribute to the enlightenment of the most important doubts about breastfeeding.Embora o leite materno seja o melhor alimento para a criança, é notável o desconhecimento das mães sobre a importância da amamentação. Foram objetivos do estudo: (1) avaliar o conhecimento de mães e gestantes acerca da amamentação e (2) elaborar uma cartilha que contemplasse as principais dúvidas identificadas. Um estudo qualitativo foi idealizado e desenvolvido por alunos de graduação em Medicina da UFBA, no curso da disciplina de Pediatria Preventiva e Social. Estudou-se uma amostra de conveniência, não probabilística, composta por 24 mães e gestantes. Utilizou-se questionário semi-estruturado, elaborado pelos autores, para entrevista pessoal. O grupo estudado apresentou diversas dúvidas e desconhecimentos acerca do tema, destacando-se aqueles relacionados ao preparo da mama e posição de amamentar (70,8%), além do momento correto para introdução de novos alimentos (67,0%). O uso de chá, água e sucos antes do sexto mês é ainda muito freqüente (50,0%) entre os lactentes, e 25,0% das mulheres temiam não ter leite suficiente. A partir desses dados, elaborou-se a cartilha informativa. Verificou-se que o nível de informação das mães sobre a amamentação é insuficiente, apesar das campanhas veiculadas pela mídia e do avanço no conhecimento científico sobre o tema. A construção de uma cartilha informativa deve contribuir no esclarecimento das principais dúvidas acerca de amamentação

Therapeutic activity of lipoxin A4 in TiO2-induced arthritis in mice: NF-κB and Nrf2 in synovial fluid leukocytes and neuronal TRPV1 mechanisms

BackgroundLipoxin A4 (LXA4) has anti-inflammatory and pro-resolutive roles in inflammation. We evaluated the effects and mechanisms of action of LXA4 in titanium dioxide (TiO2) arthritis, a model of prosthesis-induced joint inflammation and pain.MethodsMice were stimulated with TiO2 (3mg) in the knee joint followed by LXA4 (0.1, 1, or 10ng/animal) or vehicle (ethanol 3.2% in saline) administration. Pain-like behavior, inflammation, and dosages were performed to assess the effects of LXA4in vivo.ResultsLXA4 reduced mechanical and thermal hyperalgesia, histopathological damage, edema, and recruitment of leukocytes without liver, kidney, or stomach toxicity. LXA4 reduced leukocyte migration and modulated cytokine production. These effects were explained by reduced nuclear factor kappa B (NFκB) activation in recruited macrophages. LXA4 improved antioxidant parameters [reduced glutathione (GSH) and 2,2-azino-bis 3-ethylbenzothiazoline-6-sulfonate (ABTS) levels, nuclear factor erythroid 2-related factor 2 (Nrf2) mRNA and Nrf2 protein expression], reducing reactive oxygen species (ROS) fluorescent detection induced by TiO2 in synovial fluid leukocytes. We observed an increase of lipoxin receptor (ALX/FPR2) in transient receptor potential cation channel subfamily V member 1 (TRPV1)+ DRG nociceptive neurons upon TiO2 inflammation. LXA4 reduced TiO2‐induced TRPV1 mRNA expression and protein detection, as well TRPV1 co-staining with p-NFκB, indicating reduction of neuronal activation. LXA4 down-modulated neuronal activation and response to capsaicin (a TRPV1 agonist) and AITC [a transient receptor potential ankyrin 1 (TRPA1) agonist] of DRG neurons.ConclusionLXA4 might target recruited leukocytes and primary afferent nociceptive neurons to exert analgesic and anti-inflammatory activities in a model resembling what is observed in patients with prosthesis inflammation

Pervasive gaps in Amazonian ecological research

Biodiversity loss is one of the main challenges of our time,1,2 and attempts to address it require a clear un derstanding of how ecological communities respond to environmental change across time and space.3,4 While the increasing availability of global databases on ecological communities has advanced our knowledge of biodiversity sensitivity to environmental changes,5–7 vast areas of the tropics remain understudied.8–11 In the American tropics, Amazonia stands out as the world’s most diverse rainforest and the primary source of Neotropical biodiversity,12 but it remains among the least known forests in America and is often underrepre sented in biodiversity databases.13–15 To worsen this situation, human-induced modifications16,17 may elim inate pieces of the Amazon’s biodiversity puzzle before we can use them to understand how ecological com munities are responding. To increase generalization and applicability of biodiversity knowledge,18,19 it is thus crucial to reduce biases in ecological research, particularly in regions projected to face the most pronounced environmental changes. We integrate ecological community metadata of 7,694 sampling sites for multiple or ganism groups in a machine learning model framework to map the research probability across the Brazilian Amazonia, while identifying the region’s vulnerability to environmental change. 15%–18% of the most ne glected areas in ecological research are expected to experience severe climate or land use changes by 2050. This means that unless we take immediate action, we will not be able to establish their current status, much less monitor how it is changing and what is being lostinfo:eu-repo/semantics/publishedVersio