6 research outputs found
Methods to Crystallize RNA
Preparation of suitably large and wellâ ordered single crystals is usually the rateâ limiting step in the determination of the threeâ dimensional structure of RNAs and their complexes with proteins by Xâ ray crystallography. This unit discusses a variety of experimental considerations for obtaining crystals of RNAs and RNAâ protein complexes. Topics include design of crystallizable constructs, screening, and optimization of crystallization conditions.Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/152900/1/cpnc0706.pd