Tackling the cell wall of the grape berry

Abstract: The cell wall (CW) is the dynamic border of plant cells. In grape berries, the CW decisively accounts for the difference between the pulp and skin cells, with direct consequences on the grape characteristics, wine quality and wine-making methods. The softening of mature berries results from the depolymerisation and solubilisation of CW polymers. Modifications of grape pulp and skin CW provide the flexibility for cell expansion during fruit growth and to modulate the final texture. Wine making and berry processing methods are directly related with the absence, in white wines, or the presence, in red wines, of skin CW in the fermenting must. Anthocyanin extraction depends directly on skin yielding of the pigment upon CW degradation. During fruit growth and ripening, the cooperative action between different enzyme families is capital in CW metabolism. The sequencing and public availability of the Vitis genome allowed us to focus on individual pathways, to profile the expression pattern of isoforms associated with each tissue, developmental phase or stress response, anticipating the effects on berry (and wine) production and quality. Retrieving the sequences of genomic coding regions and the predicted enzymes that act on the Vitis, CW allows us for the first time to tackle the grape berry Cell Wallom

Relating water deficiency to berry texture, skin cell wall composition and expression of remodeling genes in two Vitis vinifera varieties

The cell wall (CW) is a dynamic structure that responds to stress. Water shortage (WS) impacts grapevine berry composition and its sensorial quality. In the present work, berry texture, skin CW composition, and expression of remodeling genes were investigated in two V. vinifera varieties, Touriga Nacional (TN) and Trincadeira (TR), under two water regimes, Full Irrigation (FI) and No Irrigation (NI). The global results allowed an evident separation between both varieties and the water treatments. WS resulted in increased anthocyanin contents in both varieties, reduced amounts in cellulose and lignin at maturation, but an increase in arabinose-containing polysaccharides more tightly bound to the CW in TR. In response to WS, the majority of the CW related genes were down-regulated in a variety dependent pattern. The results support the assumption that WS affects grape berries by stiffening the CW through alteration in pectin structure, supporting its involvement in responses to environmental conditions

Transgenic rat with overproduction of ubiquitous angiotensin-(1-7) presents neuroprotection in a model of ischemia and reperfusion

Recent studies showed that angiotensin-(1-7) has cerebroprotective actions in stroke. In the present study, we aim to test whether tissue overexpression of Angiotensin-(1-7), mainly in the brain provides neuroprotection in a model of ischemia/reperfusion by bilateral common carotid arteries occlusion/reperfusion (BCCAo/R). Evaluation of neurological deficit scores and bilateral asymmetry test (BAT) were performed seven days after transient BCCAo/R in transgenic rats (TG-7371) overexpressing Angiotensin-(1-7) and Sprague-Dawley (SD) rats. To assess blood-brain barrier (BBB) permeability Evans blue dye (EB) was intravenously injected. Cytokine levels were quantified in the whole brain through Elisa assay and oxidative stress was measured 7 days after ischemia. The expression of AT(1) and Mas receptors and inducible nitric oxide synthase (iNOS) was evaluated by RT-PCR. Neurological deficits were observed in both SD-BCCAo/R and TG-BCCAo/R, contrasting to sham-operated groups. However, TG-BCCAo/R showed a significant lower neurological score and latency in BAT when compared with SD-BCCAo/R. BBB integrity in TG-BCCAo/R was improved, since these animals showed lower extravasation of EB than SD-BCCAo/R. Interestingly, TG-BCCAo/R presented lower levels of pro-inflammatory cytokines when compared to SD-BCCAo/R. Levels of IL-10 were higher in SD-BCCAo/R than in SD control and even higher in TG-BCCAo/R. TG-BCCAo/R animals presented decreased levels of TBARS and increase in SOD activity and GSH levels when compared to SD sham rats. RT-PCR results showed higher levels of AT(1) receptor and iNOS in SD-BCCAo/R compared to TG-BCCAo/R, but no difference was observed for Mas receptor. The present study shows that lifetime increase in cerebral expression of an Ang-(1-7)-producing fusion protein induces neuroprotection in experimental global cerebral ischemia and reperfusion, reassuring that, pharmacological strategies leading to increase in Ang-(1-7) can be an additional tool for stroke therapy

Convex central configurations of the 4-body problem with two pairs of equal masses

Agraïments: The first and third authors are partially supported by FAPEMIG grant APQ-001082/14. The third author is partially supported by CNPq grant 472321/2013-7 and by FAPEMIG grant PPM-00516-15. The second and third autors are supported by CAPES CSF-PVE grant 88881.030454/2013-01.MacMillan and Bartky in 1932 proved that there is a unique isosceles trapezoid central configuration of the 4--body problem when two pairs of equal masses are located at adjacent vertices. After this result the following conjecture was well known between people working on central configurations: The isosceles trapezoid is the unique convex central configuration of the planar 4--body problem when two pairs of equal masses are located at adjacent vertices. We prove this conjecture

Características produtivas e nutricionais do pasto em sistema agrossilvipastoril, conforme a distância das árvores

Os objetivos deste trabalho foram avaliar os efeitos de árvores dispostas em renques sobre as características produtivas e nutricionais de Urochloa decumbens e identificar a distância média, na qual ocorre o melhor aproveitamento do sombreamento moderado, em um sistema agrossilvipastoril. Os tratamentos consistiram em distâncias dos pontos de observação ao renque das árvores (0, 3, 6, 9, 12 e 15 m) e foram distribuídos em blocos ao acaso, com quatro repetições. A densidade de perfilhos e a massa e a taxa de acúmulo de forragem e a composição química do pasto foram avaliadas. A densidade de perfilhos, a massa e a taxa de acúmulo de forragem aumentaram com a distância do renque, com valores máximos de 392 perfilhos m-2, 1.347 kg ha-1 e 30,1 kg ha-1 por dia, respectivamente, a 9,1, 10,4 e 8,8 m de distância. Os teores de fibra em detergente neutro não variaram com a distância, enquanto os de fibra em detergente ácido e os de lignina variaram, mas sem um padrão definido de resposta. Os teores de proteína bruta diminuíram quadraticamente com a distância do renque e apresentaram valor máximo sob a copa das árvores (9,8%) e mínimo a 13,5 m de distância (6,5%). A região entre 7 e 10 m de distância do renque apresentou maior benefício da sombra moderada sobre as características do pasto