The Consequences of Violence During Pregnancy for Both Fetus and Newborn: Systematic Review / Implicações para o Feto e Recém-Nascido da Violência Durante a Gestação: Revisão Sistemática

Objetivo: Identificar estudos sobre os efeitos para o feto e recém-nascido da violência contra a mulher durante a gestação. Métodos: Revisão realizada nas bases MEDLINE e LILACS. Foram incluídos estudos epidemiológicos analíticos, publicados em inglês, espanhol e português. Resultados: Foram incluídos 27 estudos. Os dados apontam na direção de que a violência na gravidez é risco para a ocorrência de restrição no crescimento intrauterino, nascer pequeno para idade gestacional e maiores níveis plasmáticos de Glutamato, GABA e cortisol. Foi consenso entre os estudos o risco de óbito fetal e neonatal entre as mulheres que vivenciaram o fenômeno da violência no período gestacional. Conclusão: A violência durante a gestação constitui um agravo relevante na saúde pública. Os achados destacam a importância da realização e inicio precoce do pré-natal, pois constitui um espaço importante no rastreamento das violências.

The methylation patterns of the IGF2 and IGF2R genes in bovine spermatozoa are not affected by flow-cytometric sex sorting

The objectives of this study were to investigate the effect of sexing by flow cytometry on the methylation patterns of the IGF2 and IGF2R genes. Frozen-thawed, unsorted, and sex-sorted sperm samples from four Nellore bulls were used. Each ejaculate was separated into three fractions: non-sexed (NS), sexed for X-sperm (SX), and sexed for Y-sperm (SY). Sperm were isolated from the extender, cryoprotectant, and other cell types by centrifugation on a 40:70% Percoll gradient, and sperm pellets were used for genomic DNA isolation. DNA was used for analyses of the methylation patterns by bisulfite sequencing. Methylation status of the IGF2 and IGF2R genes were evaluated by sequencing 195 and 147 individual clones, respectively. No global differences in DNA methylation were found between NS, SX, and SY groups for the IGF2 (P=0.09) or IGF2R genes (P=0.38). Very specific methylation patterns were observed in the 25th and 26th CpG sites in the IGF2R gene. representing higher methylation in NS than in the SX and SY groups compared with the other CpG sites. Further, individual variation in methylation patterns was found among bulls. In conclusion, the sex-sorting procedure by flow cytometry did not affect the overall DNA methylation patterns of the IGF2 and IGF2R genes, although individual variation in their methylation patterns among bulls was observed. Mol. Reprod. Dev. 79:7784, 2012. (C) 2011 Wiley Periodicals, Inc.Embrapa MP1 [003.0709040000]Embrapa MP1FAPESP [2010/15054-0]FAPESPEmbrapaEmbrapaFAPESP of BrazilFAPESP of Brazi