293 research outputs found

    Periodismo: referente comunicativo en la Sociedad de la Información

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    La ponencia parte de la siguiente hipótesis: periodismo: referente comunicativo en la Sociedad de la Información. Referente entendido en su acepción de modelo o prototipo, y en este caso de mensaje comunicativo e informativo para describir, explicar, interpretar y dar sentido a la actualidad y a la realidad comunicada de la que se ocupa el periodismo

    Underground parking structure built with deep foundations and vault precast elements in Spain

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    In many cases the only places available for the construction of a new car park are the existing streets or roads. These streets may also have important or historic buildings very close to the structure, which means that they cannot be disturbed in any way during the construction of the parking structure. In many cases the only places available for the construction of a new car park are the existing streets or roads. These streets may also have important or historic buildings very close to the structure, which means that they cannot be disturbed in any way during the construction of the parking structure

    When invasion biology meets taxonomy: Clavelina oblonga (Ascidiacea) is an old invader in the Mediterranean Sea

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    Taxonomic issues often confound the study of invasive species, which sometimes are unrecognized as introduced in newly colonized areas. Clavelina oblonga Herdman, 1880 is an abundant ascidian species along the southeastern coast of the United States and the Caribbean Sea. It was introduced into the eastern Atlantic and Brazil decades ago. In the Mediterranean Sea, a similar species had been described as C. phlegraea Salfi 1929 and reported from southern Italy and Corsica. In the last few years a species of Clavelina has proliferated in the embayments of the Ebro Delta (NW Mediterranean), a zone of active bivalve culture industry where it has smothered mussel spat, leading to economic loss. We here report the morphological and genetic identity of this species, synonymizing the Atlantic C. oblonga and the Mediterranean C. phlegraea (the latter therefore is a synonym of the former). Thus, C. oblonga has existed in the Mediterranean for over 80 years, but was known under a different name. We also found this species in natural habitats in the Iberian Atlantic coast close to the Strait of Gibraltar, raising concerns about an ongoing expansion. In order to obtain information relevant for management, we monitored growth, reproductive cycles and settlement patterns of this ascidian on bivalve cultures in the Ebro Delta. Its biological cycles were markedly seasonal, with peak abundance and reproduction during the warmest months, followed by regression during the cold season. The settlement period was short, mostly concentrated in a single month each year. Avoidance of mussel and oyster seeding during late summer and early autumn can readily reduce the damage caused by this species

    Environmental salinity modifies mucus exudation and energy use in European Sea Bass Juveniles

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    The European sea bass (Dicentrarchus labrax) is a euryhaline marine teleost that can often be found in brackish and freshwater or even in hypersaline environments. Here, we exposed sea bass juveniles to sustained salinity challenges for 15 days, simulating one hypoosmotic (3‰), one isosmotic (12‰) and one hyperosmotic (50‰) environment, in addition to control (35‰). We analyzed parameters of skin mucus exudation and mucus biomarkers, as a minimally invasive tool, and plasma biomarkers. Additionally, Na+/K+ -ATPase activity was measured, as well as the gill mucous cell distribution, type and shape. The volume of exuded mucus increased significantly under all the salinity challenges, increasing by 130% at 50‰ condition. Significantly greater amounts of soluble protein (3.9 ± 0.6 mg at 50‰ vs. 1.1 ± 0.2 mg at 35‰, p < 0.05) and lactate (4.0 ± 1.0 µg at 50‰ vs. 1.2 ± 0.3 µg at 35‰, p < 0.05) were released, with clear energy expenditure. Gill ATPase activity was significantly higher at the extreme salinities, and the gill mucous cell distribution was rearranged, with more acid and neutral mucin mucous cells at 50‰. Skin mucus osmolality suggested an osmoregulatory function as an ion-trap layer in hypoosmotic conditions, retaining osmosis-related ions. Overall, when sea bass cope with different salinities, the hyperosmotic condition (50‰) demanded more energy than the extreme hypoosmotic condition.UIDB/04326/2020, ALG-01-0145-FEDER-022121,info:eu-repo/semantics/publishedVersio

    Seasonal patterns of settlement and growth of introduced and native ascidians in bivalve cultures in the Ebro Delta (NE Iberian Peninsula)

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    Ascidians are important both as invasive species and as a fouling group in artificial marine habitats, causing negative impacts in aquaculture settings and the surrounding environment. The Ebro Delta is one of the major centres of bivalve production in the Mediterranean and is affected by proliferation of ascidian species (mostly introduced forms). Knowledge of the patterns of settlement and growth of the fouling species is mandatory to attempt mitigation measures. Settlement PVC plates were deployed from May to September 2015 at different depths (0.2, 1 and 2 m) in the Ebro Delta oyster aquaculture facilities. The occurrences of all species and the area cover of a selected subset of 6 species were monitored on a monthly basis from June 2015 to December 2016. Fifteen species were found, of which 10 are introduced. There were some differences between the deployed plates and the oyster ropes in species abundance and composition, likely due to differences in substrate complexity. For instance, Didemnum vexillum and Clavelina oblonga occurred in few plates in contrast to their abundance on oysters. The most abundant species were Styela plicata and Clavelina lepadiformis, which together with Ecteinascidia turbinata showed a preference to grow on plates deployed in May and June. Most of the species grew more at 0.2 m depth than at deeper plates. Thus, to minimise fouling on bivalves, spat immersion during fall and below 1 m depth is recommended. The number of occurrences and cover of the species was found to be similarly informative; suggesting that a periodic monitoring of species occurrence on replicate plates is sufficient for detecting new introduced species as soon as possible and will provide information useful for management

    A Trypanosoma cruzi Genome Tandem Repetitive Satellite DNA Sequence as a Molecular Marker for a LAMP Assay for Diagnosing Chagas' Disease

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    Chagas' disease is a neglected tropical disease caused by Trypanosoma cruzi which is endemic throughout Latin America and is spread by worldwide migration. Diagnosis is currently limited to serological and molecular techniques having variations regarding their sensitivity and specificity. This work was aimed at developing a new sensitive, applicable, and cost-effective molecular diagnosis technique for loop-mediated isothermal amplification-based detection of T. cruzi (Tc-LAMP). The results led to determining a highly homologous satellite repeat region (231 bp) among parasite strains as a molecular marker for diagnosing the disease. Tc-LAMP was performed correctly for detecting parasite DNA (5 fg for the CL Brener strain and 50 fg for the DM28, TcVI, and TcI strains). Assay results proved negative for DNA from 16 helminth species and 7 protozoa, including Leishmania spp. Tc-LAMP based on the highly repeated T. cruzi satellite region is thus proposed as an important alternative for diagnosing T. cruzi infection, overcoming other methods' limitations such as their analytic capability, speed, and requiring specialized equipment or highly trained personnel. Tc-LAMP could be easily adapted for point-of-care testing in areas having limited resources

    Effect of agronomic and environmental factors on CO2 emissions on a dryland rotation

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    Agriculture is a substantial source of greenhouse gas emissions (GHG) in many countries. Conservation agriculture includes soil management systems that help to reduce CO2 emission levels. However, there are many factors involved in the production of these emissions such as soil management type and time at which the agriculture operations are performed, crop phenological state, the weather, and handling of the residue amongst others. In the long term, the relationships that exist between these factors seem to determine the balance of these emissions. In this study, we analyzed the influence of the soil management system as well as the climatology of the different seasons studied and the phenological state of the different crops implanted. For this purpose a field trial was conducted in Las Cabezas de San Juán (Seville). This pilot farm consisted of six experimental plots with an approximate area of 5 ha; conservation agriculture practices were employed in three of the six plots while traditional tillage management was used in the other three. Within these plots the three crops of the wheat-sunflower-legume rotation were tested simultaneously. The study was conducted over four agricultural seasons - 2009/10, 2010/11, 2011/12 and 2012/13. Each of these cropping seasons were characterised by very different rainfall amounts, registering a total of 814.4, 721.6, 268.2 and 676.4 l/m2, respectively. When we studied the evolution of emissions over four seasons, an increase could be observed for both management systems during the time in which the crops were established due to the roots respiration processes. These increases were heavily influenced by the rainfall recorded during the time in which the crop was in place. In the case of wheat, higher emissions were produced during the cultivation time of the first and fourth season during which 84% and 60% of the total rainfall of each season was recorded. These emissions were 9 and 5 kg CO2/ha for conventional tillage and no tillage, respectively for the 2009/10 season and 11.7 and 6.8 kg CO2/ha, respectively in the 2012/13 season. Conversely during the 2011/12 season, a season in which lower precipitation was registered, the higher emissions were comparatively minor with respect to the previous values, specifically 3.7 and 1.9 kg CO2/ha for non-tillage and conventional tillage

    Evaluation of an acute osmotic stress in European sea bass via skin mucus biomarkers

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    European sea bass is a marine teleost which can inhabit a broad range of environmental salinities. So far, no research has studied the physiological response of this fish to salinity challenges using modifications in skin mucus as a potential biological matrix. Here, we used a skin mucus sampling technique to evaluate the response of sea bass to several acute osmotic challenges (for 3 h) from seawater (35¿) to two hypoosmotic environments, diluted brackish water (3¿) and estuarine waters (12¿), and to one hyperosmotic condition (50¿). For this, we recorded the volume of mucus exuded and compared the main stress-related biomarkers and osmosis-related parameters in skin mucus and plasma. Sea bass exuded the greatest volume of skin mucus with the highest total contents of cortisol, glucose, and protein under hypersalinity. This indicates an exacerbated acute stress response with possible energy losses if the condition is sustained over time. Under hyposalinity, the response depended on the magnitude of the osmotic change: shifting to 3¿ was an extreme salinity change, which affected fish aerobic metabolism by acutely modifying lactate exudation. All these data enhance the current scarce knowledge of skin mucus as a target through which to study environmental changes and fish status

    Modulation of pituitary response by dietary lipids and throughout a temperature fluctuation challenge in Gilthead Sea Bream

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    Low temperatures provoke drastic reductions in gilthead sea bream (Sparus aurata) activity and nourishment, leading to growth arrest and a halt in production. However, scarce data exist concerning the implications of central core control during the cold season. The aim of this work was to study the effects of low temperature and recovery from such exposure on the pituitary activity of sea bream juveniles fed 18% or 14% dietary lipid. A controlled indoor trial was performed to simulate natural temperature fluctuation (22 ◦C to 14 ◦C to 22 ◦C). Meanwhile, we determined the regulatory role of the pituitary by analyzing the gene expression of some pituitary hormones and hormone receptors via qPCR, as well as plasma levels of thyroidal hormones. In response to higher dietary lipids, hormone pituitary expressions were up-regulated. Induced low temperatures and lower ingesta modulated pituitary function up-regulating GH and TSH and thyroid and glucocorticoid receptors. All these findings demonstrate the capacity of the pituitary to recognize both external conditions and to modulate its response accordingly. However, growth, peripheral tissues and metabolism were not linked or connected to pituitary function at low temperatures, which opens an interesting field of study to interpret the hypothalamus–pituitary–target axis during temperature fluctuations in fish.info:eu-repo/semantics/publishedVersio
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