Next-Generation Crispr Gene-Drive Systems Using Cas12A Nuclease

One method for reducing the impact of vector-borne diseases is through the use of CRISPR-based gene drives, which manipulate insect populations due to their ability to rapidly propagate desired genetic traits into a target population. However, all current gene drives employ a Cas9 nuclease that is constitutively active, impeding our control over their propagation abilities and limiting the generation of alternative gene drive arrangements. Yet, other nucleases such as the temperature sensitive Cas12a have not been explored for gene drive designs in insects. to address this, we herein present a proof-of-concept gene-drive system driven by Cas12a that can be regulated via temperature modulation. Furthermore, we combined Cas9 and Cas12a to build double gene drives capable of simultaneously spreading two independent engineered alleles. The development of Cas12a-mediated gene drives provides an innovative option for designing next-generation vector control strategies to combat disease vectors and agricultural pests

Understanding the Role of Coordinatively Unsaturated Al3+ Sites on Nanoshaped Al2O3 for Creating Uniform Ni–Cu Alloys for Selective Hydrogenation of Acetylene

Acknowledgments This work was financially supported by the National Key R&D Program of China (2021YFB3801600), the National Natural Science Foundation of China (22218017), and the Fundamental Research Funds for the Central Universities (buctrc201921, JD2223). We acknowledge the Beijing Synchrotron Radiation Facility (BSRF) for providing the experimental resources for XAS experiments.Peer reviewedPostprin

CRISPR-based gene drives generate super-Mendelian inheritance in the disease vector Culex quinquefasciatus

Culex mosquitoes pose a significant public health threat as vectors for a variety of diseases including West Nile virus and lymphatic filariasis, and transmit pathogens threatening livestock, companion animals, and endangered birds. Rampant insecticide resistance makes controlling these mosquitoes challenging and necessitates the development of new control strategies. Gene drive technologies have made significant progress in other mosquito species, although similar advances have been lagging in Culex. Here we test a CRISPR-based homing gene drive for Culex quinquefasciatus, and show that the inheritance of two split-gene-drive transgenes, targeting different loci, are biased in the presence of a Cas9-expressing transgene although with modest efficiencies. Our findings extend the list of disease vectors where engineered homing gene drives have been demonstrated to include Culex alongside Anopheles and Aedes, and pave the way for future development of these technologies to control Culex mosquitoes

G-Protein Coupled Receptors (GPCRs): Signaling Pathways, Characterization, and Functions in Insect Physiology and Toxicology

G-protein-coupled receptors (GPCRs) are known to play central roles in the physiology of many organisms. Members of this seven α-helical transmembrane protein family transduce the extracellular signals and regulate intracellular second messengers through coupling to heterotrimeric G-proteins, adenylate cyclase, cAMPs, and protein kinases. As a result of the critical function of GPCRs in cell physiology and biochemistry, they not only play important roles in cell biology and the medicines used to treat a wide range of human diseases but also in insects’ physiological functions. Recent studies have revealed the expression and function of GPCRs in insecticide resistance, improving our understanding of the molecular complexes governing the development of insecticide resistance. This article focuses on the review of G-protein coupled receptor (GPCR) signaling pathways in insect physiology, including insects’ reproduction, growth and development, stress responses, feeding, behaviors, and other physiological processes. Hormones and polypeptides that are involved in insect GPCR regulatory pathways are reviewed. The review also gives a brief introduction of GPCR pathways in organisms in general. At the end of the review, it provides the recent studies on the function of GPCRs in the development of insecticide resistance, focusing in particular on our current knowledge of the expression and function of GPCRs and their downstream regulation pathways and their roles in insecticide resistance and the regulation of resistance P450 gene expression. The latest insights into the exciting technological advances and new techniques for gene expression and functional characterization of the GPCRs in insects are provided

Preparation of Spherical Cellulose Nanocrystals from Microcrystalline Cellulose by Mixed Acid Hydrolysis with Different Pretreatment Routes

Spherical cellulose nanocrystal (CNC), as a high value cellulose derivative, shows an excellent application potential in biomedicine, food packaging, energy storage, and many other fields due to its special structure. CNC is usually prepared by the mixed acid hydrolysis method from numerous cellulose raw materials. However, the pretreatment route in preparing spherical CNC from cellulose fiber is still used when choosing microcrystalline cellulose (MCC) as the raw material, which is not rigorous and economical. In this work, pretreatment effects on the properties of spherical CNC produced from MCC by mixed acid hydrolysis were systematically studied. Firstly, the necessity of the swelling process in pretreatment was examined. Secondly, the form effects of pretreated MCC (slurry or powder form) before acid hydrolysis in the preparation of spherical CNC were carefully investigated. The results show that the swelling process is not indispensable. Furthermore, the form of pretreated MCC also has a certain influence on the morphology, crystallinity, and thermal stability of spherical CNC. Thus, spherical CNC with different properties can be economically prepared from MCC by selecting different pretreatment routes through mixed acid hydrolysis

Development of 0.34 THz Sub-Harmonic Mixer Combining Two-Stage Reduced Matching Technology with an Improved Active Circuit Model

In this paper, a high-performance 0.34 THz sub-harmonic mixer combining two-stage reduced matching technology with an improved active circuit model is established and analyzed. The mixer’s improved active circuit model is realized by decomposing passive functional models into basic transmission line units and their impedance matching and filtering are realized through automatic optimization. The improved active circuit model takes out the RF (radio frequency) transition model separately to set an optimization goal instead of operating directly in the mixing circuit. Compared with traditional active circuit models in the SDM (subdivision design method) and GDM (global design method), it provides a massive optimization space, larger working bandwidth, and better results. In the RF frequency range of 320–360 GHz, the SSB (single sideband) conversion loss of the 0.34 THz sub-harmonic mixer is below 9.5 dB and the RF return loss is less than 12 dB

Development of 0.34 THz Sub-Harmonic Mixer Combining Two-Stage Reduced Matching Technology with an Improved Active Circuit Model

In this paper, a high-performance 0.34 THz sub-harmonic mixer combining two-stage reduced matching technology with an improved active circuit model is established and analyzed. The mixer’s improved active circuit model is realized by decomposing passive functional models into basic transmission line units and their impedance matching and filtering are realized through automatic optimization. The improved active circuit model takes out the RF (radio frequency) transition model separately to set an optimization goal instead of operating directly in the mixing circuit. Compared with traditional active circuit models in the SDM (subdivision design method) and GDM (global design method), it provides a massive optimization space, larger working bandwidth, and better results. In the RF frequency range of 320–360 GHz, the SSB (single sideband) conversion loss of the 0.34 THz sub-harmonic mixer is below 9.5 dB and the RF return loss is less than 12 dB

Multiple-P450 Gene Co-Up-Regulation in the Development of Permethrin Resistance in the House Fly, <i>Musca domestica</i>

This paper reports a study conducted at the whole transcriptome level to characterize the P450 genes involved in the development of pyrethroid resistance, utilizing expression profile analyses of 86 cytochrome P450 genes in house fly strains with different levels of resistance to pyrethroids/permethrin. Interactions among the up-regulated P450 genes and possible regulatory factors in different autosomes were examined in house fly lines with different combinations of autosomes from a resistant house fly strain, ALHF. Eleven P450 genes that were significantly up-regulated, with levels > 2-fold those in the resistant ALHF house flies, were in CYP families 4 and 6 and located on autosomes 1, 3 and 5. The expression of these P450 genes was regulated by trans- and/or cis-acting factors, especially on autosomes 1 and 2. An in vivo functional study indicated that the up-regulated P450 genes also conferred permethrin resistance in Drosophila melanogaster transgenic lines. An in vitro functional study confirmed that the up-regulated P450 genes are able to metabolize not only cis- and trans-permethrin, but also two metabolites of permethrin, PBalc and PBald. In silico homology modeling and the molecular docking methodology further support the metabolic capacity of these P450s for permethrin and substrates. Taken together, the findings of this study highlight the important function of multi-up-regulated P450 genes in the development of insecticide resistance in house flies

Identifying sectoral impacts on global scarce water uses from multiple perspectives

Scarce water uses driven by hotspots in production and consumption stages of global supply chains have been well studied. However, hotspots in primary inputs and intermediate transmission stages also leading to large amounts of global scarce water uses are overlooked. This gap can lead to the underestimation of the impacts of certain nation sectors on global scarce water uses. This study identifies critical primary suppliers and transmission centers in global supply chains contributing to scarce water uses, based on environmentally extended multi‐regional input‐output (EE‐MRIO) model and complex network analysis methods. Results show that some critical primary suppliers (e.g., the service auxiliary to financial intermediation sector in the United States and the financial intermediation services sector in India) and transmission centers (e.g., the raw milk sector in the United States and the transmission services of electricity sector in China) are unidentifiable in previous studies. These findings provide hotspots for supply‐side measures (e.g., optimization of primary input and product allocation behaviors) and productivity improvement measures. The critical inter‐sectoral transactions (mainly involving the agricultural and food products sectors in India, China, and the United States) further provide explicit directions for these measures. Moreover, this study conducts a community detection, which identifies communities (i.e., the clusters of nation sectors closely interconnected) leading to global scarce water uses. Most of the communities involve sectors from different nations, providing foundations for international cooperation strategies.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/171255/1/jiec13171.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/171255/2/jiec13171_am.pd