The Bacterial Photosynthetic Reaction Center as a Model for Membrane Proteins

Membrane proteins participate in many fundamental cellular processes. Until recently, an understanding of the function and properties of membrane proteins was hampered by an absence of structural information at the atomic level. A landmark achievement toward understanding the structure of membrane proteins was the crystallization (1) and structure determination (2-5) the photosynthetic reaction center (RC) from the purple bacteria Rhodopseudomonas viridis, followed by that of the RC from Rhodobacter sphaeroides (6-17). The RC is an integral membrane protein-pigment complex, which carries out the initial steps of photosynthesis (reviewed in 18). RCs from the purple bacteria Rps. viridis and Rb. sphaeroides are composed of three membrane-associated protein subunits (designated L, M, and H), and the following cofactors: four bacteriochlorophylls (Bchl or B), two bacteriopheophytins (Bphe or [phi]), two quinones, and a nonheme iron. The cofactors are organized into two symmetrical branches that are approximately related by a twofold rotation axis (2, 8). A central feature of the structural organization of the RC is the presence of 11 hydrophobic [alpha]-helixes, approximately 20-30 residues long, which are believed to represent the membrane-spanning portion of the RC (3, 9). Five membrane-spanning helixes are present in both the L and M subunits, while a single helix is in the H subunit. The folding of the L and M subunits is similar, consistent with significant sequence similarity between the two chains (19-25). The L and M subunits are approximately related by the same twofold rotation axis that relates the two cofactor branches. RCs are the first membrane proteins to be described at atomic resolution; consequently they provide an important model for discussing the folding of membrane proteins. The structure demonstrates that [alpha]-helical structures may be adopted by integral membrane proteins, and provides confirmation of the utility of hydropathy plots in identifying nonpolar membrane-spanning regions from sequence data. An important distinction between the folding environments of water-soluble proteins and membrane proteins is the large difference in water concentration surrounding the proteins. As a result, hydrophobic interactions (26) play very different roles in stabilizing the tertiary structures of these two classes of proteins; this has important structural consequences. There is a striking difference in surface polarity of membrane and water-soluble proteins. However, the characteristic atomic packing and surface area appear quite similar. A computational method is described for defining the position of the RC in the membrane (10). After localization of the RC structure in the membrane, surface residues in contact with the lipid bilayer were identified. As has been found for soluble globular proteins, surface residues are less well conserved in homologous membrane proteins than the buried, interior residues. Methods based on the variability of residues between homologous proteins are described (13); they are useful (a) in defining surface helical regions of membrane and water-soluble proteins and (b) in assigning the side of these helixes that are exposed to the solvent. A unifying view of protein structure suggests that water-soluble proteins may be considered as modified membrane proteins with covalently attached polar groups that solubilize the proteins in aqueous solution

Hidden symmetries of a self-dual monopole

The symmetries of a spinning particle in the field of a self-dual monopole are studied from the viewpoint of supersymmetric quantum mechanics.Comment: Originally appeared in: Symmetries in Science III, Proc. Schloss Hofen Meeting, Gruber and Iachello (eds.), pp. 525-529. Plenum (1989). Available in inspire (code {Feher:1988ym}). Typos corrected and reference list updated. 7 page

Interplay between mesoscopic phase separation and bulk magnetism in the layered NaxCoO2

Specific heat of the layered NaxCoO2 (x=0.65, 0.70 and 0.75) oxides has been measured in the temperature range of 3-360 K and magnetic field of 0 and 9 T. The analysis of data, assuming the combined effect of inter-layer superexchange and the phase separation into mesoscopic magnetic domains with localized spins embedded in a matrix with itinerant electronic character, suggests that the dominant contribution to the specific heat in the region of short-range ordering is mediated by quasi-2D antiferromagnetic clusters, perpendicular to the CoO2 layers

Dynamical supersymmetry of spin particle-magnetic field interaction

We study the super and dynamical symmetries of a fermion in a monopole background. The Hamiltonian also involves an additional spin-orbit coupling term, which is parameterized by the gyromagnetic ratio. We construct the superinvariants associated with the system using a SUSY extension of a previously proposed algorithm, based on Grassmann-valued Killing tensors. Conserved quantities arise for certain definite values of the gyromagnetic factor: $\N=1$ SUSY requires $g=2$; a Kepler-type dynamical symmetry only arises, however, for the anomalous values $g=0$ and $g=4$. The two anomalous systems can be unified into an $\N=2$ SUSY system built by doubling the number of Grassmann variables. The planar system also exhibits an $\N=2$ supersymmetry without Grassmann variable doubling.Comment: 23 page

Finite W Algebras and Intermediate Statistics

New realizations of finite W algebras are constructed by relaxing the usual constraint conditions. Then, finite W algebras are recognized in the Heisenberg quantization recently proposed by Leinaas and Myrheim, for a system of two identical particles in d dimensions. As the anyonic parameter is directly associated to the W-algebra involved in the d=1 case, it is natural to consider that the W-algebra framework is well-adapted for a possible generalization of the anyon statistics.Comment: 16 pp., Latex, Preprint ENSLAPP-489/9

A Magnetic Resonance Force Microscopy Quantum Computer with Tellurium Donors in Silicon

We propose a magnetic resonance force microscopy (MRFM)-based nuclear spin quantum computer using tellurium impurities in silicon. This approach to quantum computing combines the well-developed silicon technology with expected advances in MRFM.Comment: 9 pages, 1 figur