Cluster observations of the midaltitude cusp under strong northward interplanetary magnetic field

We report on a multispacecraft cusp observation lasting more than 100 min. We determine the cusp boundary motion and reveal the effect on the cusp size of the interplanetary magnetic field (IMF) changing from southward to northward. The cusp shrinks at the beginning of the IMF rotation and it reexpands at the rate of 0.40° invariant latitude per hour under stable northward IMF. On the basis of plasma signatures inside the cusp, such as counterstreaming electrons with balanced fluxes, we propose that pulsed dual lobe reconnection operates during the time of interest. SC1 and SC4 observations suggest a long-term regular periodicity of the pulsed dual reconnection, which we estimate to be ~1–5 min. Further, the distances from the spacecraft to the reconnection site are estimated on the basis of observations from three satellites. The distance determined using SC1 and SC4 observations is ~15 RE and that determined from SC3 data is ~8 RE. The large-scale speed of the reconnection site sunward motion is ~16 km s-1. We observe also a fast motion of the reconnection site by SC1, which provides new information about the transitional phase after the IMF rotation. Finally, a statistical study of the dependency of plasma convection inside the cusp on the IMF clock angle is performed. The relationship between the cusp stagnation, the dual lobe reconnection process, and the IMF clock angle is discussed

Chemoselective Suzuki-Miyaura cross-coupling via kinetic transmetallation

Chemoselective Suzuki-Miyaura cross-coupling generally requires a designed deactivation of one nucleophile towards transmetallation. Here we show that boronic acids can be chemoselectively reacted in the presence of ostensibly equivalently reactive boronic acid pinacol (BPin) esters by kinetic discrimination during transmetallation. Simultaneous electrophile control allows sequential chemoselective cross-couplings in a single operation in the absence of protecting groups

Lymphoproliferative disease in mice infected with murine gammaherpesvirus 68

Murine gammaherpesvirus is a natural pathogen of wild rodents. In the laboratory it establishes an infection of epithelial cells and persists in B lymphocytes in a latent form. Inbred mice chronically infected with the virus develop a lymphoproliferative disease (LPD) similar to that seen in patients infected with Epstein-Barr virus. The frequency of LPD over a period of 3 years was 9% of all infected animals, with 50% of these displaying high grade lymphomas. The incidence of LPD was greatly increased when infected mice were treated with cyclosporin A. The majority of mice used in the experiments were BALB/c, although lymphomas were detected in mice on other genetic backgrounds, ie, CBA and B10Br. Lymphomas were associated with both lymphoid and nonlymphoid tissues (liver, lung, and kidney). In all cases of lymphomas studied thus far, there was a mixed B cell (B220+ve) and T cell (CD3+ve) phenotype. The B cells were light chain restricted, indicative of a clonal origin. Variable numbers of virus genome-positive cells were detected by in situ hybridization in and around the lymphomas. In contrast, no lytic antigen-positive cells were detected, indicating that genome-positive cells were either latently infected or undergoing an abortive infection. These observations suggest that murine gammaherpesvirus-infected mice may be an important model to study the pathogenesis of LPD associated with other gammaherpesviruses, such as Epstein-Barr virus

Electron velocity distribution and lion roars in the magnetosheath

International audienceWhistler waves which are termed "lion roars" in the magnetosheath are studied using data obtained by the Spectrum Analyser (SA) of the Spatio-Temporal Analysis of Field Fluctuations (STAFF) experiment aboard Cluster. Kinetic theory is then employed to obtain the theoretical expression for the whistler wave with electron temperature anisotropy which is believed to trigger lion roars in the magnetosheath. This allows us to compare theory and data. This paper for the first time studies the details of the electron velocity distribution function as measured by the Plasma Electron And Current Experiment (PEACE) in order to investigate the underlying causes for the different types of lion roars found in the data. Our results show that while some instances of lion roars could be locally generated, the source of others must be more remote regions of the magnetosheath

Kelvin-Helmholtz multi-spacecraft studies at the Earth's magnetopause boundaries

Copyright © 2010 American Institute of Physics. This article may be downloaded for personal use only. Any other use requires prior permission of the author and the American Institute of Physics.The following article appeared in AIP Conference Proceedings Volume 1216, pp. 483-486, and may be found at http://dx.doi.org/10.1063/1.3395908Twelfth International Solar Wind Conference, Saint‐Malo, France, 21–26 June 2009The Kelvin‐Helmholtz (KH) instability can operate in various situations in the solar wind, but at the boundaries of planetary obstacles, for example the Earth’s magnetopause, it is most amenable to investigation. Reliable estimates of wave characteristics are essential for comparison with theoretical and numerical models and for understanding the nonlinear development of KH waves and their role in the plasma entry into the magnetosphere. After discussing their typical conditions of appearance in KH unstable domains at the magnetopause, both theoretically and observationally, we outline recent results of multi‐spacecraft analysis with Cluster giving accurate, albeit spatially limited, determination of surface wave characteristics. Those characteristics (wavelength and propagation direction), close to the terminator on the nightside, are likely to be prescribed by the 3‐D geometry and the bending of field lines developed by the KH waves, rather than by the magnitude and the direction of the magnetosheath or background flow. An unprecedented number of satellites provides now the opportunity to extend the analysis of source regions of KH waves and their domains of development

Evolution of Kelvin-Helmholtz activity on the dusk flank magnetopause

Copyright © 2008 American Geophysical Union (AGU)Our purpose is to characterize the evolution of the magnetopause Kelvin-Helmholtz (KH) wave activity with changes in thickness of the adjacent boundary layer, geomagnetic latitude and interplanetary magnetic field (IMF) orientation. As the IMF turns northward, wave activity may be generated at the dayside before propagating down the tail, where the boundary layer is expected to support longer wavelengths. We use two-point observations on the dusk magnetopause at low latitudes, from Geotail on the dayside and Cluster tailward of the dusk terminator. We quantify the wavelength, power, wavefront steepness and propagation direction at Cluster. An estimate of the thickness of the low-latitude boundary layer (LLBL) is obtained by correlating normal distances to the magnetopause, derived from two empirical solar-wind-driven models, with a systematic relationship (the “transition parameter”) found between the electron number density and temperature; the correlation factor is used to infer the temporal evolution of the thickness of the locally sampled layer. We find that wavelengths are controlled by the IMF clock angle, as expected when generated by the KH mechanism at the dayside, although amplitudes, wavefront steepness and propagation directions are more closely correlated with the layer thickness. A survey of parameter space provides evidence of the contribution of the KH mechanism to the widening of the electron LLBL

The Chemistry of the Triterpenes and Related Compounds. Part XXXII.* The Chemistry of Hydroxyhopanone

The functional groups of hydroxyhopanone, a saturated C30H50O2 pentacyclic triterpene keto-alcohol have been characterised and a tentative structure for hydroxyhopanone is proposed

Statistical study of the location and size of the electron edge of the Low-Latitude Boundary Layer as observed by Cluster at mid-altitudes

The nature of particle precipitations at dayside mid-altitudes can be interpreted in terms of the evolution of reconnected field lines. Due to the difference between electron and ion parallel velocities, two distinct boundary layers should be observed at mid-altitudes between the boundary between open and closed field lines and the injections in the cusp proper. At lowest latitudes, the electron-dominated boundary layer, named the "electron edge" of the Low-Latitude Boundary Layer (LLBL), contains soft-magnetosheath electrons but only high-energy ions of plasma sheet origin. A second layer, the LLBL proper, is a mixture of both ions and electrons with characteristic magnetosheath energies. The Cluster spacecraft frequently observe these two boundary layers. We present an illustrative example of a Cluster mid-altitude cusp crossing with an extended electron edge of the LLBL. This electron edge contains 10–200 eV, low-density, isotropic electrons, presumably originating from the solar wind halo population. These are occasionally observed with bursts of parallel and/or anti-parallel-directed electron beams with higher fluxes, which are possibly accelerated near the magnetopause X-line. We then use 3 years of data from mid-altitude cusp crossings (327 events) to carry out a statistical study of the location and size of the electron edge of the LLBL. We find that the equatorward boundary of the LLBL electron edge is observed at 10:00–17:00 magnetic local time (MLT) and is located typically between 68° and 80° invariant latitude (ILAT). The location of the electron edge shows a weak, but significant, dependence on some of the external parameters (solar wind pressure, and IMF <i>B<sub>Z</sub></i>- component), in agreement with expectations from previous studies of the cusp location. The latitudinal extent of the electron edge has been estimated using new multi-spacecraft techniques. The Cluster tetrahedron crosses the electron and ion boundaries of the LLBL/cusp with time delays of 1–40 min between spacecraft. We reconstruct the motion of the electron boundary between observations by different spacecraft to improve the accuracy of the estimation of the boundary layer size. In our study, the LLBL electron edge is distinctly observed in 87% of mid-altitude LLBL/cusp crossings with clear electron and ion equatorward boundaries equivalent to 35% of all LLBL/cusp crossings by Cluster. The size of this region varied between 0°–2° ILAT with a median value of 0.2° ILAT. Generally, the size of the LLBL electron edge depends on the combination of many parameters. However, we find an anti-correlation between the size of this region and the strength of the IMF, the absolute values of the IMF <i>B<sub>Y</sub></i>- and <i>B<sub>Z</sub></i>-components and the solar wind dynamic pressure, as is expected from a simple reconnection model for the origin of this region

On the location of dayside magnetic reconnection during an interval of duskward oriented IMF

We present space- and ground-based observations of the signatures of magnetic reconnection during an interval of duskward-oriented interplanetary magnetic field on 25 March 2004. In situ field and plasma measurements are drawn from the Double Star and Cluster satellites during traversals of the pre-noon sector dayside magnetopause at low and high latitudes, respectively. These reveal the typical signatures of flux transfer events (FTEs), namely bipolar perturbations in the magnetic field component normal to the local magnetopause, enhancements in the local magnetic field strength and mixing of magnetospheric and magnetosheath plasmas. Further evidence of magnetic reconnection is inferred from the ground-based signatures of pulsed ionospheric flow observed over an extended interval. In order to ascertain the location of the reconnection site responsible for the FTEs, a simple model of open flux tube motion over the surface of the magnetopause is employed. A comparison of the modelled and observed motion of open flux tubes (i.e. FTEs) and plasma flow in the magnetopause boundary layer indicates that the FTEs observed at both low and high latitudes were consistence with the existence of a tilted X-line passing through the sub-solar region, as suggested by the component reconnection paradigm. While a high latitude X-line (as predicted by the anti-parallel description of reconnection) may have been present, we find it unlikely that it could have been responsible for the FTEs observed in the pre-noon sector under the observed IMF conditions. Finally, we note that throughout the interval, the magnetosphere was bathed in ULF oscillations within the solar wind electric field. While no one-to-one correspondence with the pulsed reconnection rate suggested by the ground-based observation of pulsed ionospheric flow has been demonstrated, we note that similar periodicity oscillations were observed throughout the solar wind-magnetosphere-ionosphere system. These findings are consistent with previously proposed mechanisms of solar wind modulation of the dayside reconnection rate

Untargeted metabolomics reveals a new mode of action of pretomanid (PA-824)

Pretomanid is a promising anti-tubercular drug currently at clinical phase III, but its mechanisms of action are currently unclear. This study aimed to: (i) reveal the metabolome of Mycobacterium smegmatis under pretomanid treatment; (ii) compare major sources of metabolite variation in bacteria treated with pretomanid treatment and other antibiotics; and (iii) to target metabolites responsible for the killing activity of pretomanid in mycobacteria. Untargeted high-resolution metabolite profiling was carried out using flow infusion electrospray ion high resolution mass spectrometry (FIE-HRMS) to identify and quantify metabolites. The identification of key metabolites was independently confirmed by gas-chromatography time-of flight mass spectrometry (GC-tofMS) in comparison to standards. Pretomanid treatments generated a unique distinctive metabolite profile when compared to ampicillin, ethambutol, ethionamide, isoniazid, kanamycin, linezolid, rifampicin and streptomycin. Metabolites which differed significantly only with pretomanid treatment were identified and mapped on to bacterial metabolic pathways. This targeted the pentose phosphate pathway with significant accumulation seen with fructose-6-phosphate, ribose-5-phosphate and glyceraldehyde-3-phosphate. These effects were linked to the accumulation of a toxic metabolite methylglyoxal. This compound showed significant antimicrobial activity (MIC 0.65 mM) against M. smegmatis.publishersversionPeer reviewe