15 research outputs found
Development of a genomic evaluation for milk production for a local bovine breed
DairySN
New insights on the mechanism of quinoline-based DNA methyltransferase inhibitors
Among the epigenetic marks, DNA methylation is one of the most studied. It is highly deregulated in numerous diseases, including cancer. Indeed, it has been shown that hypermethylation of tumor suppressor genes promoters is a common feature of cancer cells. Because DNA methylation is reversible, the DNA methyltransferases (DNMTs), responsible for this epigenetic mark, are considered promising therapeutic targets. Several molecules have been identified as DNMT inhibitors and, among the non-nucleoside inhibitors, 4-aminoquinoline-based inhibitors, such as SGI-1027 and its analogs, showed potent inhibitory activity. Here we characterized the in vitro mechanism of action of SGI-1027 and two analogs. Enzymatic competition studies with the DNA substrate and the methyl donor cofactor, S-adenosyl-L-methionine (AdoMet), displayed AdoMet non-competitive and DNA competitive behavior. In addition, deviations from the Michaelis-Menten model in DNA competition experiments suggested an interaction with DNA. Thus their ability to interact with DNA was established; although SGI-1027 was a weak DNA ligand, analog 5, the most potent inhibitor, strongly interacted with DNA. Finally, as 5 interacted with DNMT only when the DNA duplex was present, we hypothesize that this class of chemical compounds inhibit DNMTs by interacting with the DNA substrate
Direct use of MACE EBV in the Walloon single-step Bayesian genomic evaluation system
DairySN
Convention de collaboration technique et scientifique dans le cadre des évaluations génétiques bovines laitières pour la Wallonie. Rapport d’activités pour l’année 2013
Development of a CERT START Domain–Ceramide HTRF Binding Assay and Application to Pharmacological Studies and Screening
International audienc
Rapport d'activités final du second mandat (du 1er janvier 2012 au 31 août 2014): DairySNP, Etude de la variabilité génomique des bovins laitiers et mixtes en vue de mieux connaître leur biodiversité et d'initier une sélection génomique au sein de ces races
D31-127
Rapport d'activités final du premier mandat (du 1er janvier 2010 au 31 décembre 2011) : DairySNP, Etude de la variabilité génomique des bovins laitiers et mixtes en vue de mieux connaître leur biodiversité et d'initier une sélection génomique au sein de ces races
D31-1233 : DairySN