Učinci 4-vinilcikloheksen diepoksida na testise pasa

This study was aimed at investigating the effects of 4-vinylcyclohexene diepoxide (VCD) on testicular tissue in dogs. For this purpose, 30 mongrel male dogs were used and allocated to 5 groups randomly. The first group (n = 6), used as the control, was administered sesame oil by the intraperitoneal route (i/p). The VCD was administered i/p at doses of 80, 160, 240, 320 mg/kg to the second, third, fourth and the fifth groups (n = 6), respectively, once a day for 8 days. The dogs were surgically castrated on the day after the last VCD injection. The testes were removed immediately and fixed in 10% bouine solution. The testes were subjected to routine tissue processing and examined to determine the testicular damage in each testis. When compared to the control group, it was seen that the seminiferous tubule damage increased significantly in the treatment groups. Malondialdehyde levels reached the highest level in the fifth group, while superoxide dismutase, catalase, glutathione peroxidase activity and glutathione levels were higher in the control group (P0.05). Our results showed that VCD may contribute to the sterilization of male dogs.Cilj ovoga istraživanja bio je ispitati učinak 4-vinilcikloheksen diepoksida (VCD) na tkivo testisa u pasa. U tu je svrhu 30 mužjaka, križanaca, slučajnim odabirom podijeljeno u pet skupina. Prvoj, kontrolnoj skupini (n = 6) dan je VCD sa sezamovim uljem intraperitonealno. Druga skupina primila je VCD intraperitonealno u dozi od 80 mg/kg, treća 160 mg/kg, četvrta 240 mg/kg i peta skupina 320 mg/kg (n = 6), jedanput dnevno tijekom 8 dana. Psi su kastrirani dan nakon posljednje injekcije VCD-a. Testisi su odmah uklonjeni i stavljeni u 10 %-tni Bouineov fiksativ. Podvrgnuti su rutinskoj obradi tkiva te ispitani da se utvrde oštećenja na svakom testisu. U usporedbi s kontrolnom skupinom uočeno je da je u pokusnoj skupini oštećenje sjemenovoda značajno veće. Razine malondialdehida najveće su vrijednosti dosegnule u petoj skupini, dok je aktivnost superoksidne dismutaze, katalaze i glutation-peroksidaze bila veća u kontrolnoj skupini (P0,05). Rezultati pokazuju da VCD može pridonijeti sterilitetu kod muških pasa