A new approach for achievement of inulin accumulation in suspension cultures of Jerusalem artichoke (Helianthus tuberosus) using biotic elicitors

AbstractA promising protocol for achievement the accumulation rate of inulin compound in a suspension culture of Jerusalem artichoke (Helianthus tuberosus) was established. The effect of incorporated of cell cultures in combining with two type of biotic elicitors Aspergillus niger extract and Methyl-Jasmonate incorporation feeding medium on leaf cell growth patterns and production of inulin was investigated. The maximum value of cell growth parameters and highest content of inulinase activity (0.395u/ml) were resulted from elicitation of augmented MS-medium with A. niger extract at the level of 0.2% in combination with Methyl-Jasmonate (150μM) as compared with other concentrations after 2weeks of cultivation. The chemical analyses of the different cell lines were spectro-photometerically performed. This study clearly indicates that combining of A. niger and Methyl-Jasmonate elicitors plays a critical role on inulin process and its accumulation in Jerusalem artichoke cell cultures

ORIGINAL ARTICLES Effect of Heavy Metal (HgCl 2 ) on Accumulation and Production of Total Indole Alkaloids, Vinblastine And/or Vincristine from Egyptian Catharanthus Roseus

ABSTRACT Catharanthus roseus is still the only source for the powerful antitumor drugs vinblastine and vincristine. Effects of mercuric (HgCl 2 ) on enhancement the accumulation rate of total indole alkaloids; antineoplastic agents (vinblastine and/or vincristine) in calli cultures of Egyptian Catharanthus roseus (L.) G. (Don) were studied. Hgcl 2 was used at levels of 0.0, 0.2, 0.4 and 0.8 μm/l, added to modified alkaloid production medium (MAPM). The obtained results showed that: All tested concentrations of Hg significantly decreased the different measured growth parameters of shoot and root derived calli, however, the percentage of dry matter content significantly increased comparing the control treatment. Moreover, the percentage of total indole-alkaloids was significantly increased. The highest values of the accumulation rate of total indole-alkaloids, further, vinblastine and/or vincristine were recorded at the rate of 0.8 mM / Hg in shoot and root derived calli, respectively