4 research outputs found
PIN1 as a Predictive Biomarker for H. pylori Infection–Associated Gastric Cancer
Cancer is now one of the major causes of death across the globe. Peptidylprolyl cis/trans isomerase, NIMA-interacting 1 (PIN1), has recently emerged as a critical factor in various cancers. Numerous studies have shown that PIN1 is highly expressed in several cancer types and is significantly associated with the prognosis of patients with a certain type of tumor such as gastric cancer. Meanwhile, some studies have indicated that infection with Helicobacter pylori significantly increases the risk of developing duodenal and gastric ulcer disease and gastric cancer. In this article, we propose that PIN1 can play a vital role in the prognosis of Helicobacter pylori infection-associated with peptic ulcer disease and can be effective in order to provide the best cure and the choice for treatment
Cytotoxic effects of hydroalcoholic extract of Cuscuta chinensis on PC3 and MCF7 cancer cell lines
Objective: Chemoprevention of cancer by application of natural phytochemical compounds has been used to prevent, delay or suppress cancer progression. Cuscuta chinensis a traditional Iranian medicinal herb, has biological properties including anticancer, anti-aging, immuno-stimulatory and antioxidant effects. In this study, anti-proliferative effects of hydroalcoholic extract of C. chinensis on prostate (PC3) and breast (MCF7) cancer cell lines were investigated. Materials and Methods: In the current study, we investigated treatment of PC3 cells with different concentrations of C. chinensis (0, 100, 200, 300, 400, and 500 µg/ml) for 24 and 48 hr; also, MCF7 cells were treated with various concentrations (0-600 µg/ml) of C. chinensis for 48 and 72 hr and cell viability was assessed by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. mRNA expression of BCL2 Associated X (Bax), B-cell lymphoma 2 (Bcl2), Cysteine-aspartic proteases (Caspase3) and Phosphatase and tensin homolog (PTEN) were analyzed by quantitative real-time PCR. Annexin V/PI staining and lactate dehydrogenase (LDH) cytotoxicity assay were used to detect apoptosis. Results: C. chinensis decreased PC3 and MCF7 cells viability in a dose- and time-dependent manner (p BAX/Bcl2 ratio, Caspase3 and PTEN increased in C. chinensis-treated cells compared to the control group. C. chinensis induced apoptosis (p <0.001) and LDH activity (p Conclusion: Our findings suggest that C. chinensis extract is able to inhibit proliferation and induce apoptosis in PC3 and MCF7 cell lines. Therefore, C. chinensis extract exerts antitumor activity against cancer cells